Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region
The pathogenicity of four isolates of infectious bursal disease virus (IBDV) that have restriction fragment length polymorphism patterns of very virulent IBDV (vvIBDV), based on the presence of SspI and TaqI sites in the VP2 hypervariable region, was studied in specific pathogen free chickens. Chick...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2001
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| Online Access: | http://psasir.upm.edu.my/id/eprint/40375/1/Pathogenicity%20of%20SspI-positive%20infectious%20bursal%20disease%20virus%20and%20molecular%20characterization%20of%20the%20VP2%20hypervariable%20region.pdf |
| _version_ | 1796973728657047552 |
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| author | Hoque, Mahfuzul M. Omar, Abdul Rahman Chong, Lee Kim Bejo, Mohd Hair Ideris, Aini |
| author_facet | Hoque, Mahfuzul M. Omar, Abdul Rahman Chong, Lee Kim Bejo, Mohd Hair Ideris, Aini |
| author_sort | Hoque, Mahfuzul M. |
| collection | UPM |
| description | The pathogenicity of four isolates of infectious bursal disease virus (IBDV) that have restriction fragment length polymorphism patterns of very virulent IBDV (vvIBDV), based on the presence of SspI and TaqI sites in the VP2 hypervariable region, was studied in specific pathogen free chickens. Chickens inoculated with isolates 92/04, 94/B551 and 97/61 developed severe clinical signs with a high mortality ranging from 70 to 80%, whereas the 94/273 isolate caused 10% mortality. Regardless of the isolates, significant differences were noted in the bursal lesion scores and bursa:body weight ratio index in the infected groups in comparison with the control groups. However, the presence of lesions in non-bursal tissues, muscles, thymus and at the junction of the proventriculus and gizzard were found only in the 92/04, 97/61 and 94/B551 isolates. Restriction fragment length polymorphism and sequence analysis of the VP2 hypervariable region indicated that all the isolates can be classified as vvIBDV based on the presence of SspI and TaqI sites at nucleotide positions 1011 and 833, respectively. In addition, all the isolates had amino acid substitutions at P222A, V256I and L294I, which are characteristic for vvIBDV isolated from different parts of the world. All the isolates except 94/273 also had a StyI site at nucleotide position 888. The absence of a StyI site in this isolate was associated with amino acid substitution at 254 from G to S. The 94/273 also had an amino acid substitution at position 270 from A to E, which is variable in the STC, Cu1 and OH strains. The presence of amino acid substitutions from G254S andA270E in SspI- and TaqI-positive vvIBDV strains is very uncommon and has not been reported previously. These amino acid variations might have caused the 94/273 to become less virulent in specific pathogen free chickens and resemble a classical virulent IBDV strain. |
| first_indexed | 2024-03-06T08:46:53Z |
| format | Article |
| id | upm.eprints-40375 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:46:53Z |
| publishDate | 2001 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-403752015-09-17T08:41:12Z http://psasir.upm.edu.my/id/eprint/40375/ Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region Hoque, Mahfuzul M. Omar, Abdul Rahman Chong, Lee Kim Bejo, Mohd Hair Ideris, Aini The pathogenicity of four isolates of infectious bursal disease virus (IBDV) that have restriction fragment length polymorphism patterns of very virulent IBDV (vvIBDV), based on the presence of SspI and TaqI sites in the VP2 hypervariable region, was studied in specific pathogen free chickens. Chickens inoculated with isolates 92/04, 94/B551 and 97/61 developed severe clinical signs with a high mortality ranging from 70 to 80%, whereas the 94/273 isolate caused 10% mortality. Regardless of the isolates, significant differences were noted in the bursal lesion scores and bursa:body weight ratio index in the infected groups in comparison with the control groups. However, the presence of lesions in non-bursal tissues, muscles, thymus and at the junction of the proventriculus and gizzard were found only in the 92/04, 97/61 and 94/B551 isolates. Restriction fragment length polymorphism and sequence analysis of the VP2 hypervariable region indicated that all the isolates can be classified as vvIBDV based on the presence of SspI and TaqI sites at nucleotide positions 1011 and 833, respectively. In addition, all the isolates had amino acid substitutions at P222A, V256I and L294I, which are characteristic for vvIBDV isolated from different parts of the world. All the isolates except 94/273 also had a StyI site at nucleotide position 888. The absence of a StyI site in this isolate was associated with amino acid substitution at 254 from G to S. The 94/273 also had an amino acid substitution at position 270 from A to E, which is variable in the STC, Cu1 and OH strains. The presence of amino acid substitutions from G254S andA270E in SspI- and TaqI-positive vvIBDV strains is very uncommon and has not been reported previously. These amino acid variations might have caused the 94/273 to become less virulent in specific pathogen free chickens and resemble a classical virulent IBDV strain. Taylor & Francis 2001 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40375/1/Pathogenicity%20of%20SspI-positive%20infectious%20bursal%20disease%20virus%20and%20molecular%20characterization%20of%20the%20VP2%20hypervariable%20region.pdf Hoque, Mahfuzul M. and Omar, Abdul Rahman and Chong, Lee Kim and Bejo, Mohd Hair and Ideris, Aini (2001) Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region. Avian Pathology, 30 (4). pp. 369-380. ISSN 0307-9457; ESSN: 1465-3338 http://www.tandfonline.com/doi/abs/10.1080/03079450120066377 10.1080/03079450120066377 |
| spellingShingle | Hoque, Mahfuzul M. Omar, Abdul Rahman Chong, Lee Kim Bejo, Mohd Hair Ideris, Aini Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region |
| title | Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region |
| title_full | Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region |
| title_fullStr | Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region |
| title_full_unstemmed | Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region |
| title_short | Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region |
| title_sort | pathogenicity of sspi positive infectious bursal disease virus and molecular characterization of the vp2 hypervariable region |
| url | http://psasir.upm.edu.my/id/eprint/40375/1/Pathogenicity%20of%20SspI-positive%20infectious%20bursal%20disease%20virus%20and%20molecular%20characterization%20of%20the%20VP2%20hypervariable%20region.pdf |
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