Excretion of purine derivatives in cows: endogenous contribution and recovery of exogenous purine bases
Two dry cows fitted with simple cannula in the rumen and duodenum, and fed with a mixed diet (straw:barley, 50:50), were used to determine endogenous losses and response of urinary purine derivatives (PDs) to duodenally infused yeast-RNA. Duodenal flow of purine bases (PBs) was determined by a dual...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier
2001
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| Online Access: | http://psasir.upm.edu.my/id/eprint/40377/1/Excretion%20of%20purine%20derivatives%20in%20cows%20endogenous%20contribution%20and%20recovery%20of%20exogenous%20purine%20bases.pdf http://psasir.upm.edu.my/id/eprint/40377/7/1-s2.0-S0301622600002311-main.pdf |
| _version_ | 1811137440900972544 |
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| author | Boero, P. Orellana Balcells, Joaquim Martin-Orue, Susana Maria Liang, Juan Boo Guada, Jose Antonio |
| author_facet | Boero, P. Orellana Balcells, Joaquim Martin-Orue, Susana Maria Liang, Juan Boo Guada, Jose Antonio |
| author_sort | Boero, P. Orellana |
| collection | UPM |
| description | Two dry cows fitted with simple cannula in the rumen and duodenum, and fed with a mixed diet (straw:barley, 50:50), were used to determine endogenous losses and response of urinary purine derivatives (PDs) to duodenally infused yeast-RNA. Duodenal flow of purine bases (PBs) was determined by a dual marker system, and 15N was infused continuously into the rumen to label microbial PBs. The isotope dilution of urinary PDs in relation to duodenal PBs confirmed the presence of an endogenous fraction (236 μmol/kg LW0.75) bigger than in sheep and lower than values estimated in cows with impaired rumen fermentation. Excretion of PDs increased linearly in response to incremental supply of PBs with an equimolar recovery of 0.84 mol/mol. However, net recovery of duodenal PBs was 0.67 for the basal diet and 0.65, 0.90, 0.79 and 0.82 for the four levels of PB infusion. It is suggested that differences in digestibility between yeast-RNA and duodenal PBs might explain differences in recovery estimations. |
| first_indexed | 2024-03-06T08:46:54Z |
| format | Article |
| id | upm.eprints-40377 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-09-25T03:34:20Z |
| publishDate | 2001 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-403772024-07-26T07:53:27Z http://psasir.upm.edu.my/id/eprint/40377/ Excretion of purine derivatives in cows: endogenous contribution and recovery of exogenous purine bases Boero, P. Orellana Balcells, Joaquim Martin-Orue, Susana Maria Liang, Juan Boo Guada, Jose Antonio Two dry cows fitted with simple cannula in the rumen and duodenum, and fed with a mixed diet (straw:barley, 50:50), were used to determine endogenous losses and response of urinary purine derivatives (PDs) to duodenally infused yeast-RNA. Duodenal flow of purine bases (PBs) was determined by a dual marker system, and 15N was infused continuously into the rumen to label microbial PBs. The isotope dilution of urinary PDs in relation to duodenal PBs confirmed the presence of an endogenous fraction (236 μmol/kg LW0.75) bigger than in sheep and lower than values estimated in cows with impaired rumen fermentation. Excretion of PDs increased linearly in response to incremental supply of PBs with an equimolar recovery of 0.84 mol/mol. However, net recovery of duodenal PBs was 0.67 for the basal diet and 0.65, 0.90, 0.79 and 0.82 for the four levels of PB infusion. It is suggested that differences in digestibility between yeast-RNA and duodenal PBs might explain differences in recovery estimations. Elsevier 2001-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/40377/1/Excretion%20of%20purine%20derivatives%20in%20cows%20endogenous%20contribution%20and%20recovery%20of%20exogenous%20purine%20bases.pdf text en http://psasir.upm.edu.my/id/eprint/40377/7/1-s2.0-S0301622600002311-main.pdf Boero, P. Orellana and Balcells, Joaquim and Martin-Orue, Susana Maria and Liang, Juan Boo and Guada, Jose Antonio (2001) Excretion of purine derivatives in cows: endogenous contribution and recovery of exogenous purine bases. Livestock Production Science, 68 (2-3). pp. 243-250. ISSN 0301-6226 http://www.sciencedirect.com/science/article/pii/S0301622600002311 10.1016/S0301-6226(00)00231-1 |
| spellingShingle | Boero, P. Orellana Balcells, Joaquim Martin-Orue, Susana Maria Liang, Juan Boo Guada, Jose Antonio Excretion of purine derivatives in cows: endogenous contribution and recovery of exogenous purine bases |
| title | Excretion of purine derivatives in cows: endogenous contribution and recovery of exogenous purine bases |
| title_full | Excretion of purine derivatives in cows: endogenous contribution and recovery of exogenous purine bases |
| title_fullStr | Excretion of purine derivatives in cows: endogenous contribution and recovery of exogenous purine bases |
| title_full_unstemmed | Excretion of purine derivatives in cows: endogenous contribution and recovery of exogenous purine bases |
| title_short | Excretion of purine derivatives in cows: endogenous contribution and recovery of exogenous purine bases |
| title_sort | excretion of purine derivatives in cows endogenous contribution and recovery of exogenous purine bases |
| url | http://psasir.upm.edu.my/id/eprint/40377/1/Excretion%20of%20purine%20derivatives%20in%20cows%20endogenous%20contribution%20and%20recovery%20of%20exogenous%20purine%20bases.pdf http://psasir.upm.edu.my/id/eprint/40377/7/1-s2.0-S0301622600002311-main.pdf |
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