Cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates
This study was conducted to ascertain the cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates (OPKHs) produced from its protein isolate. A modified microplate titer WST-1 [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] assay was used to investiga...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2014
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/40781/1/9%20IFRJ%2021%20%2803%29%202014%20Chang%20713.pdf |
| _version_ | 1796973807947218944 |
|---|---|
| author | Chang, Sui Kiat Hamajima, Hiroshi Ismail, Amin Yanagita, Teruyoshi Mohd Esa, Norhaizan Baharuldin, Mohamad Taufik Hidayat |
| author_facet | Chang, Sui Kiat Hamajima, Hiroshi Ismail, Amin Yanagita, Teruyoshi Mohd Esa, Norhaizan Baharuldin, Mohamad Taufik Hidayat |
| author_sort | Chang, Sui Kiat |
| collection | UPM |
| description | This study was conducted to ascertain the cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates (OPKHs) produced from its protein isolate. A modified microplate titer WST-1 [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] assay was used to investigate the cytotoxicity of hydrolysates produced from protease and pepsin-pancreatin hydrolysis at various concentrations (0.1, 1, 10, 100 μg/ml and 1 mg/ml) using HepG2 cell model. Additionally, peptide stimulation test using OPKHs at 1 mg/ml was carried out to investigate whether OPKHs could serve as growth factor for HepG2 cells other than affecting its viability. As a result, oleic acid appeared to normalize the WST-1 readings of HepG2 cells treated with both hydrolysates at 1 mg/ml. The presence of amino acids in OPKHs could stimulate the growth and prolongs the viability of HepG2 cells. Both OPKHs were non-cytotoxic to HepG2 cells at all tested concentrations even at high concentrations. This study indicated that pepsin-pancreatin and protease hydrolysates produced from oil palm kernel protein were non-cytotoxic on HepG2 cells. |
| first_indexed | 2024-03-06T08:48:02Z |
| format | Article |
| id | upm.eprints-40781 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:48:02Z |
| publishDate | 2014 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-407812015-10-08T08:19:07Z http://psasir.upm.edu.my/id/eprint/40781/ Cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates Chang, Sui Kiat Hamajima, Hiroshi Ismail, Amin Yanagita, Teruyoshi Mohd Esa, Norhaizan Baharuldin, Mohamad Taufik Hidayat This study was conducted to ascertain the cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates (OPKHs) produced from its protein isolate. A modified microplate titer WST-1 [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] assay was used to investigate the cytotoxicity of hydrolysates produced from protease and pepsin-pancreatin hydrolysis at various concentrations (0.1, 1, 10, 100 μg/ml and 1 mg/ml) using HepG2 cell model. Additionally, peptide stimulation test using OPKHs at 1 mg/ml was carried out to investigate whether OPKHs could serve as growth factor for HepG2 cells other than affecting its viability. As a result, oleic acid appeared to normalize the WST-1 readings of HepG2 cells treated with both hydrolysates at 1 mg/ml. The presence of amino acids in OPKHs could stimulate the growth and prolongs the viability of HepG2 cells. Both OPKHs were non-cytotoxic to HepG2 cells at all tested concentrations even at high concentrations. This study indicated that pepsin-pancreatin and protease hydrolysates produced from oil palm kernel protein were non-cytotoxic on HepG2 cells. Faculty of Food Science and Technology, Universiti Putra Malaysia 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40781/1/9%20IFRJ%2021%20%2803%29%202014%20Chang%20713.pdf Chang, Sui Kiat and Hamajima, Hiroshi and Ismail, Amin and Yanagita, Teruyoshi and Mohd Esa, Norhaizan and Baharuldin, Mohamad Taufik Hidayat (2014) Cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates. International Food Research Journal, 21 (3). pp. 909-914. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/21%20%2803%29%202014/9%20IFRJ%2021%20%2803%29%202014%20Chang%20713.pdf |
| spellingShingle | Chang, Sui Kiat Hamajima, Hiroshi Ismail, Amin Yanagita, Teruyoshi Mohd Esa, Norhaizan Baharuldin, Mohamad Taufik Hidayat Cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates |
| title | Cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates |
| title_full | Cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates |
| title_fullStr | Cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates |
| title_full_unstemmed | Cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates |
| title_short | Cytotoxicity effect of oil palm (Elaeis guineensis) kernel protein hydrolysates |
| title_sort | cytotoxicity effect of oil palm elaeis guineensis kernel protein hydrolysates |
| url | http://psasir.upm.edu.my/id/eprint/40781/1/9%20IFRJ%2021%20%2803%29%202014%20Chang%20713.pdf |
| work_keys_str_mv | AT changsuikiat cytotoxicityeffectofoilpalmelaeisguineensiskernelproteinhydrolysates AT hamajimahiroshi cytotoxicityeffectofoilpalmelaeisguineensiskernelproteinhydrolysates AT ismailamin cytotoxicityeffectofoilpalmelaeisguineensiskernelproteinhydrolysates AT yanagitateruyoshi cytotoxicityeffectofoilpalmelaeisguineensiskernelproteinhydrolysates AT mohdesanorhaizan cytotoxicityeffectofoilpalmelaeisguineensiskernelproteinhydrolysates AT baharuldinmohamadtaufikhidayat cytotoxicityeffectofoilpalmelaeisguineensiskernelproteinhydrolysates |