Detection of Klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR)
Klebsiella pneumoniae (K. pneumoniae) is one of the most important members of Klebsiella genus in Enterobacteriacae family, which is responsible for pneumonia (the destructive lung inflammation disease). Vegetables are known as source of contamination with K. pneumonia. Raw vegetables are usually co...
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/40959/1/Detection%20of%20klebsiella%20pneumoniae%20in%20raw%20vegetables%20using%20most%20probable%20number-polymerase%20chain%20reaction%20%28MPN-PCR%29.pdf |
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| author | Puspanadan, Soopna Loo, Yuet Ying Nillian, Elexson Kuan, Chee Hao Goh, Sur Guat Chang, Wei San Lye, Ying Ling Afsah-Hejri, Leili Tang, John Yew Huat Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_facet | Puspanadan, Soopna Loo, Yuet Ying Nillian, Elexson Kuan, Chee Hao Goh, Sur Guat Chang, Wei San Lye, Ying Ling Afsah-Hejri, Leili Tang, John Yew Huat Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_sort | Puspanadan, Soopna |
| collection | UPM |
| description | Klebsiella pneumoniae (K. pneumoniae) is one of the most important members of Klebsiella genus in Enterobacteriacae family, which is responsible for pneumonia (the destructive lung inflammation disease). Vegetables are known as source of contamination with K. pneumonia. Raw vegetables are usually consumed in salads and other dishes. The aim of this study was to investigate the occurrence of K. pneumoniae in raw vegetables marketed in Malaysia. Two hundred commonly used salad vegetables (lettuces, parsley, cucumber, tomato and carrot) from hypermarkets and wet markets were investigated for presence of K. pneumoniae using Most Probable Number-Polymerase Chain Reaction (MPN-PCR). K. pneumoniae was found to be significantly more frequent (100%) and (82.5%) in lettuce and cucumbers, respectively. K. pneumoniae contamination was lowest in carrot samples (30%). All samples were contaminated with K. pneumoniae ranging from <3 to 1100 MPN/g. Results showed the high health risk associated with consumption of raw vegetables. |
| first_indexed | 2024-03-06T08:48:31Z |
| format | Article |
| id | upm.eprints-40959 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:48:31Z |
| publishDate | 2012 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-409592015-11-30T03:23:24Z http://psasir.upm.edu.my/id/eprint/40959/ Detection of Klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR) Puspanadan, Soopna Loo, Yuet Ying Nillian, Elexson Kuan, Chee Hao Goh, Sur Guat Chang, Wei San Lye, Ying Ling Afsah-Hejri, Leili Tang, John Yew Huat Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Klebsiella pneumoniae (K. pneumoniae) is one of the most important members of Klebsiella genus in Enterobacteriacae family, which is responsible for pneumonia (the destructive lung inflammation disease). Vegetables are known as source of contamination with K. pneumonia. Raw vegetables are usually consumed in salads and other dishes. The aim of this study was to investigate the occurrence of K. pneumoniae in raw vegetables marketed in Malaysia. Two hundred commonly used salad vegetables (lettuces, parsley, cucumber, tomato and carrot) from hypermarkets and wet markets were investigated for presence of K. pneumoniae using Most Probable Number-Polymerase Chain Reaction (MPN-PCR). K. pneumoniae was found to be significantly more frequent (100%) and (82.5%) in lettuce and cucumbers, respectively. K. pneumoniae contamination was lowest in carrot samples (30%). All samples were contaminated with K. pneumoniae ranging from <3 to 1100 MPN/g. Results showed the high health risk associated with consumption of raw vegetables. Faculty of Food Science and Technology, Universiti Putra Malaysia 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/40959/1/Detection%20of%20klebsiella%20pneumoniae%20in%20raw%20vegetables%20using%20most%20probable%20number-polymerase%20chain%20reaction%20%28MPN-PCR%29.pdf Puspanadan, Soopna and Loo, Yuet Ying and Nillian, Elexson and Kuan, Chee Hao and Goh, Sur Guat and Chang, Wei San and Lye, Ying Ling and Afsah-Hejri, Leili and Tang, John Yew Huat and Rukayadi, Yaya and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2012) Detection of Klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR). International Food Research Journal, 19 (4). pp. 1757-1762. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/19%20%2804%29%202012/64%20IFRJ%2019%20%2804%29%202012%20Sopna%20%28130%29.pdf |
| spellingShingle | Puspanadan, Soopna Loo, Yuet Ying Nillian, Elexson Kuan, Chee Hao Goh, Sur Guat Chang, Wei San Lye, Ying Ling Afsah-Hejri, Leili Tang, John Yew Huat Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Detection of Klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR) |
| title | Detection of Klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR) |
| title_full | Detection of Klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR) |
| title_fullStr | Detection of Klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR) |
| title_full_unstemmed | Detection of Klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR) |
| title_short | Detection of Klebsiella pneumoniae in raw vegetables using most probable number-polymerase chain reaction (MPN-PCR) |
| title_sort | detection of klebsiella pneumoniae in raw vegetables using most probable number polymerase chain reaction mpn pcr |
| url | http://psasir.upm.edu.my/id/eprint/40959/1/Detection%20of%20klebsiella%20pneumoniae%20in%20raw%20vegetables%20using%20most%20probable%20number-polymerase%20chain%20reaction%20%28MPN-PCR%29.pdf |
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