Detection of mycoplasma gallisepticum by real time PCR using gapA gene
Mycoplasma gallisepticum (MG) is responsible for chronic respiratory disease which leads to a lot of economic losses to the poultry industry. Therefore, it is very important to detect this organism early and efficiently. MG can be diagnosed by three ways: isolation identification, serological method...
| Main Authors: | , , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41404/1/41404.pdf |
| _version_ | 1796973930335961088 |
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| author | Yasmin, Farhana Ideris, Aini Omar, Abdul Rahman Bejo, Mohd Hair Tan, Sheau Wei Tan, Ching Giap Islam, R. Ahmad, K. |
| author_facet | Yasmin, Farhana Ideris, Aini Omar, Abdul Rahman Bejo, Mohd Hair Tan, Sheau Wei Tan, Ching Giap Islam, R. Ahmad, K. |
| author_sort | Yasmin, Farhana |
| collection | UPM |
| description | Mycoplasma gallisepticum (MG) is responsible for chronic respiratory disease which leads to a lot of economic losses to the poultry industry. Therefore, it is very important to detect this organism early and efficiently. MG can be diagnosed by three ways: isolation identification, serological method and molecular detection method. In this study, a SYBR green real time PCR assay by using gapA gene was developed for the detection of MG. This assay was powerful both from the aspect of specificity and sensitivity as it amplified only MG at early cycle when run together with 20 other avian Mycoplasma species and it can detect a minimum of 26 pg/μl of DNA template. |
| first_indexed | 2024-03-06T08:49:48Z |
| format | Conference or Workshop Item |
| id | upm.eprints-41404 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:49:48Z |
| publishDate | 2013 |
| publisher | Faculty of Veterinary Medicine, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-414042016-01-28T01:07:10Z http://psasir.upm.edu.my/id/eprint/41404/ Detection of mycoplasma gallisepticum by real time PCR using gapA gene Yasmin, Farhana Ideris, Aini Omar, Abdul Rahman Bejo, Mohd Hair Tan, Sheau Wei Tan, Ching Giap Islam, R. Ahmad, K. Mycoplasma gallisepticum (MG) is responsible for chronic respiratory disease which leads to a lot of economic losses to the poultry industry. Therefore, it is very important to detect this organism early and efficiently. MG can be diagnosed by three ways: isolation identification, serological method and molecular detection method. In this study, a SYBR green real time PCR assay by using gapA gene was developed for the detection of MG. This assay was powerful both from the aspect of specificity and sensitivity as it amplified only MG at early cycle when run together with 20 other avian Mycoplasma species and it can detect a minimum of 26 pg/μl of DNA template. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2013 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41404/1/41404.pdf Yasmin, Farhana and Ideris, Aini and Omar, Abdul Rahman and Bejo, Mohd Hair and Tan, Sheau Wei and Tan, Ching Giap and Islam, R. and Ahmad, K. (2013) Detection of mycoplasma gallisepticum by real time PCR using gapA gene. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 107-108). http://www.vet.upm.edu.my/dokumen/90301_proceeding_WPSA_V2_first_second_XX_new_20121013_%281%29.pdf |
| spellingShingle | Yasmin, Farhana Ideris, Aini Omar, Abdul Rahman Bejo, Mohd Hair Tan, Sheau Wei Tan, Ching Giap Islam, R. Ahmad, K. Detection of mycoplasma gallisepticum by real time PCR using gapA gene |
| title | Detection of mycoplasma gallisepticum by real time PCR using gapA gene |
| title_full | Detection of mycoplasma gallisepticum by real time PCR using gapA gene |
| title_fullStr | Detection of mycoplasma gallisepticum by real time PCR using gapA gene |
| title_full_unstemmed | Detection of mycoplasma gallisepticum by real time PCR using gapA gene |
| title_short | Detection of mycoplasma gallisepticum by real time PCR using gapA gene |
| title_sort | detection of mycoplasma gallisepticum by real time pcr using gapa gene |
| url | http://psasir.upm.edu.my/id/eprint/41404/1/41404.pdf |
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