Determination of avian influenza virus H5N2 infection in chicken innate immune cells
Infection with low pathogenic avian influenza virus in birds and mammals is not severe and usually give a mild clinical signs comparing to the highly pathogenic avian influenza virus. Once the virus enter the immune system of an organism, the first defense mechanism which consist of the antigen pres...
| Main Authors: | , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41406/1/41406.pdf |
| _version_ | 1825949779427852288 |
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| author | Mohd Zaini, Farhana Abdolmaleki, Mostafa Yeap, Swee Keong Tan, Sheau Wei Ideris, Aini Omar, Abdul Rahman |
| author_facet | Mohd Zaini, Farhana Abdolmaleki, Mostafa Yeap, Swee Keong Tan, Sheau Wei Ideris, Aini Omar, Abdul Rahman |
| author_sort | Mohd Zaini, Farhana |
| collection | UPM |
| description | Infection with low pathogenic avian influenza virus in birds and mammals is not severe and usually give a mild clinical signs comparing to the highly pathogenic avian influenza virus. Once the virus enter the immune system of an organism, the first defense mechanism which consist of the antigen presenting cells and NK cells will react on the virus by activating the innate and adaptive immunity system. N2 gene from H5N2 strain A/duck/Malaysia/8443/04 was detected using SYBR Green real-time PCR from the infected bone marrow dendritic cell, macrophage and also natural killer cells. Comparing the increment of viral load among 6h, 12h and 24h, there were no significant difference between the viral load detected in bone marrow dendritic cells and natural killer cells. Meanwhile, for macrophage the viral load decreased gradually over time. |
| first_indexed | 2024-03-06T08:49:48Z |
| format | Conference or Workshop Item |
| id | upm.eprints-41406 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:49:48Z |
| publishDate | 2013 |
| publisher | Faculty of Veterinary Medicine, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-414062016-01-28T01:10:31Z http://psasir.upm.edu.my/id/eprint/41406/ Determination of avian influenza virus H5N2 infection in chicken innate immune cells Mohd Zaini, Farhana Abdolmaleki, Mostafa Yeap, Swee Keong Tan, Sheau Wei Ideris, Aini Omar, Abdul Rahman Infection with low pathogenic avian influenza virus in birds and mammals is not severe and usually give a mild clinical signs comparing to the highly pathogenic avian influenza virus. Once the virus enter the immune system of an organism, the first defense mechanism which consist of the antigen presenting cells and NK cells will react on the virus by activating the innate and adaptive immunity system. N2 gene from H5N2 strain A/duck/Malaysia/8443/04 was detected using SYBR Green real-time PCR from the infected bone marrow dendritic cell, macrophage and also natural killer cells. Comparing the increment of viral load among 6h, 12h and 24h, there were no significant difference between the viral load detected in bone marrow dendritic cells and natural killer cells. Meanwhile, for macrophage the viral load decreased gradually over time. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2013 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41406/1/41406.pdf Mohd Zaini, Farhana and Abdolmaleki, Mostafa and Yeap, Swee Keong and Tan, Sheau Wei and Ideris, Aini and Omar, Abdul Rahman (2013) Determination of avian influenza virus H5N2 infection in chicken innate immune cells. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 109-110). http://www.vet.upm.edu.my/dokumen/90301_proceeding_WPSA_V2_first_second_XX_new_20121013_%281%29.pdf |
| spellingShingle | Mohd Zaini, Farhana Abdolmaleki, Mostafa Yeap, Swee Keong Tan, Sheau Wei Ideris, Aini Omar, Abdul Rahman Determination of avian influenza virus H5N2 infection in chicken innate immune cells |
| title | Determination of avian influenza virus H5N2 infection in chicken innate immune cells |
| title_full | Determination of avian influenza virus H5N2 infection in chicken innate immune cells |
| title_fullStr | Determination of avian influenza virus H5N2 infection in chicken innate immune cells |
| title_full_unstemmed | Determination of avian influenza virus H5N2 infection in chicken innate immune cells |
| title_short | Determination of avian influenza virus H5N2 infection in chicken innate immune cells |
| title_sort | determination of avian influenza virus h5n2 infection in chicken innate immune cells |
| url | http://psasir.upm.edu.my/id/eprint/41406/1/41406.pdf |
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