Phylogenetic analysis of haemaglutinin gene of avian influenza virus isolate VRI1803/04
An isolate of avian influenza virus (AIV) designated as VRI1803/04 obtained from Veterinary Research Institute, Ipoh, Perak was used for identification and characterisation using the conventional one step reverse transcription polymerase chain reaction (RT-PCR). Total RNA of the virus was obtained f...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41409/1/41409.pdf |
| Summary: | An isolate of avian influenza virus (AIV) designated as VRI1803/04 obtained from Veterinary Research Institute, Ipoh, Perak was used for identification and characterisation using the conventional one step reverse transcription polymerase chain reaction (RT-PCR). Total RNA of the virus was obtained from allantoic fluid of specific-pathogen-free (SPF) embryonated chicken eggs extracted using conventional method. The complete sequence of H3 gene (1701 bp) of VRI1803/04 was determined. The H gene has 89% homology to H3 gene from A/duck/Ukraine/1/63 (H3N8). |
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