In ovo vaccination against Newcastle disease virus using a DNA vaccine co-expressing the F and HN genes and dextran-spermin as a vehicle for DNA delivery
Newcastle disease virus (NDV) is recognized as a fatal disease virus affecting domestic and wild avian species. Different types of vaccines are under testing to ensure that there is efficient control. In the present study, a DNA vaccine, namely pIRES-HN/F, alone or in mixture with Dextran-spermine,...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Universiti Putra Malaysia
2013
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41413/1/41413.pdf |
| _version_ | 1796973932113297408 |
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| author | Firouzamandi, Masumeh Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh Ideris, Aini |
| author_facet | Firouzamandi, Masumeh Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh Ideris, Aini |
| author_sort | Firouzamandi, Masumeh |
| collection | UPM |
| description | Newcastle disease virus (NDV) is recognized as a fatal disease virus affecting domestic and wild avian species. Different types of vaccines are under testing to ensure that there is efficient control. In the present study, a DNA vaccine, namely pIRES-HN/F, alone or in mixture with Dextran-spermine, as a vehicle, was applied for in ovo vaccination to NDV. The antibody titer in the chickens immunised by the pDNA was higher compared to the chickens immunised with pDNA-Dextran complex. The produced antibodies failed to control the acute NDV infection after challenge and the severity of infection in the most of the dead chickens showed variation. Immunisation with the pDNA/Dextran-spermine nanoparticles induced low level of antibody titer compared with the pDNA alone. Therefore, it was concluded that Dextran-spermine was not effective to facilitate the entrance of pDNA into the cell by in ovo vaccination. |
| first_indexed | 2024-03-06T08:49:50Z |
| format | Conference or Workshop Item |
| id | upm.eprints-41413 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:49:50Z |
| publishDate | 2013 |
| publisher | Faculty of Veterinary Medicine, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-414132016-01-28T02:02:53Z http://psasir.upm.edu.my/id/eprint/41413/ In ovo vaccination against Newcastle disease virus using a DNA vaccine co-expressing the F and HN genes and dextran-spermin as a vehicle for DNA delivery Firouzamandi, Masumeh Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh Ideris, Aini Newcastle disease virus (NDV) is recognized as a fatal disease virus affecting domestic and wild avian species. Different types of vaccines are under testing to ensure that there is efficient control. In the present study, a DNA vaccine, namely pIRES-HN/F, alone or in mixture with Dextran-spermine, as a vehicle, was applied for in ovo vaccination to NDV. The antibody titer in the chickens immunised by the pDNA was higher compared to the chickens immunised with pDNA-Dextran complex. The produced antibodies failed to control the acute NDV infection after challenge and the severity of infection in the most of the dead chickens showed variation. Immunisation with the pDNA/Dextran-spermine nanoparticles induced low level of antibody titer compared with the pDNA alone. Therefore, it was concluded that Dextran-spermine was not effective to facilitate the entrance of pDNA into the cell by in ovo vaccination. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2013 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41413/1/41413.pdf Firouzamandi, Masumeh and Bejo, Mohd Hair and Omar, Abdul Rahman and Mehrbod, Parvaneh and Ideris, Aini (2013) In ovo vaccination against Newcastle disease virus using a DNA vaccine co-expressing the F and HN genes and dextran-spermin as a vehicle for DNA delivery. In: World’s Poultry Science Association (Malaysia Branch) and World Veterinary Poultry Association (Malaysia Branch) Scientific Conference 2013, 30 Nov.-1 Dec. 2013, Faculty of Veterinary Medicine, Universiti Putra Malaysia. (pp. 123-125). http://www.vet.upm.edu.my/dokumen/90301_proceeding_WPSA_V2_first_second_XX_new_20121013_%281%29.pdf |
| spellingShingle | Firouzamandi, Masumeh Bejo, Mohd Hair Omar, Abdul Rahman Mehrbod, Parvaneh Ideris, Aini In ovo vaccination against Newcastle disease virus using a DNA vaccine co-expressing the F and HN genes and dextran-spermin as a vehicle for DNA delivery |
| title | In ovo vaccination against Newcastle disease virus using a DNA vaccine co-expressing the F and HN genes and dextran-spermin as a vehicle for DNA delivery |
| title_full | In ovo vaccination against Newcastle disease virus using a DNA vaccine co-expressing the F and HN genes and dextran-spermin as a vehicle for DNA delivery |
| title_fullStr | In ovo vaccination against Newcastle disease virus using a DNA vaccine co-expressing the F and HN genes and dextran-spermin as a vehicle for DNA delivery |
| title_full_unstemmed | In ovo vaccination against Newcastle disease virus using a DNA vaccine co-expressing the F and HN genes and dextran-spermin as a vehicle for DNA delivery |
| title_short | In ovo vaccination against Newcastle disease virus using a DNA vaccine co-expressing the F and HN genes and dextran-spermin as a vehicle for DNA delivery |
| title_sort | in ovo vaccination against newcastle disease virus using a dna vaccine co expressing the f and hn genes and dextran spermin as a vehicle for dna delivery |
| url | http://psasir.upm.edu.my/id/eprint/41413/1/41413.pdf |
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