Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus
Background: Very virulent infectious bursal disease virus (vvIBDV) induces immunosuppression and inflammation in young birds, which subsequently leads to high mortality. In addition, infectious bursal disease (IBD) is one of the leading causes of vaccine failure on farms. Therefore, understanding th...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BioMed Central
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/41941/1/41941.pdf |
| _version_ | 1825929062652051456 |
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| author | Rasoli, Mehdi Yeap, Swee Keong Tan, Sheau Wei Roohani, Kiarash Teo, Kristeen Ye Wen Mohamed Alitheen, Noorjahan Banu Abd Rahman, Yasmin Ideris, Aini Bejo, Mohd Hair Kaiser, Pete Omar, Abdul Rahman |
| author_facet | Rasoli, Mehdi Yeap, Swee Keong Tan, Sheau Wei Roohani, Kiarash Teo, Kristeen Ye Wen Mohamed Alitheen, Noorjahan Banu Abd Rahman, Yasmin Ideris, Aini Bejo, Mohd Hair Kaiser, Pete Omar, Abdul Rahman |
| author_sort | Rasoli, Mehdi |
| collection | UPM |
| description | Background: Very virulent infectious bursal disease virus (vvIBDV) induces immunosuppression and inflammation in young birds, which subsequently leads to high mortality. In addition, infectious bursal disease (IBD) is one of the leading causes of vaccine failure on farms. Therefore, understanding the immunopathogenesis of IBDV in both the spleen and the bursae could help effective vaccine development. However, previous studies only profiled the differential expression of a limited number of cytokines, in either the spleen or the bursae of Fabricius of IBDV-infected chickens. Thus, this study aims to evaluate the in vitro and in vivo immunoregulatory effects of vvIBDV infection on macrophage-like cells, spleen and bursae of Fabricius. Results: The viral load was increased during the progression of the in vitro infection in the HD11 macrophage cell line and in vivo, but no significant difference was observed between the spleen and the bursae tissue. vvIBDV infection induced the expression of pro-inflammatory and Th1 cytokines, and chemokines from HD11 cells in a time- and dosage-dependent manner. Furthermore, alterations in the lymphocyte populations, cytokine and chemokine expression, were observed in the vvIBDV-infected spleens and bursae. A drastic rise was detected in numbers of macrophages and pro-inflammatory cytokine expression in the spleen, as early as 2 days post-infection (dpi). On 4 dpi, macrophage and T lymphocyte infiltration, associated with the peak expression of pro-inflammatory cytokines in the bursae tissues of infected chickens were observed. The majority of the significantly regulated pro-inflammatory cytokines and chemokines, in vvIBDV-infected spleens and bursae, were also detected in vvIBDV-infected HD11 cells. This cellular infiltration subsequently resulted in a sharp rise in nitric oxide (NO) and lipid peroxidation levels. Conclusion: This study suggests that macrophage may play an important role in regulating the early expression of pro-inflammatory cytokines, first in the spleen and then in the bursae, the latter tissue undergoing macrophage infiltration at 4 dpi. |
| first_indexed | 2024-03-06T08:51:22Z |
| format | Article |
| id | upm.eprints-41941 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:51:22Z |
| publishDate | 2015 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | upm.eprints-419412016-02-10T04:03:49Z http://psasir.upm.edu.my/id/eprint/41941/ Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus Rasoli, Mehdi Yeap, Swee Keong Tan, Sheau Wei Roohani, Kiarash Teo, Kristeen Ye Wen Mohamed Alitheen, Noorjahan Banu Abd Rahman, Yasmin Ideris, Aini Bejo, Mohd Hair Kaiser, Pete Omar, Abdul Rahman Background: Very virulent infectious bursal disease virus (vvIBDV) induces immunosuppression and inflammation in young birds, which subsequently leads to high mortality. In addition, infectious bursal disease (IBD) is one of the leading causes of vaccine failure on farms. Therefore, understanding the immunopathogenesis of IBDV in both the spleen and the bursae could help effective vaccine development. However, previous studies only profiled the differential expression of a limited number of cytokines, in either the spleen or the bursae of Fabricius of IBDV-infected chickens. Thus, this study aims to evaluate the in vitro and in vivo immunoregulatory effects of vvIBDV infection on macrophage-like cells, spleen and bursae of Fabricius. Results: The viral load was increased during the progression of the in vitro infection in the HD11 macrophage cell line and in vivo, but no significant difference was observed between the spleen and the bursae tissue. vvIBDV infection induced the expression of pro-inflammatory and Th1 cytokines, and chemokines from HD11 cells in a time- and dosage-dependent manner. Furthermore, alterations in the lymphocyte populations, cytokine and chemokine expression, were observed in the vvIBDV-infected spleens and bursae. A drastic rise was detected in numbers of macrophages and pro-inflammatory cytokine expression in the spleen, as early as 2 days post-infection (dpi). On 4 dpi, macrophage and T lymphocyte infiltration, associated with the peak expression of pro-inflammatory cytokines in the bursae tissues of infected chickens were observed. The majority of the significantly regulated pro-inflammatory cytokines and chemokines, in vvIBDV-infected spleens and bursae, were also detected in vvIBDV-infected HD11 cells. This cellular infiltration subsequently resulted in a sharp rise in nitric oxide (NO) and lipid peroxidation levels. Conclusion: This study suggests that macrophage may play an important role in regulating the early expression of pro-inflammatory cytokines, first in the spleen and then in the bursae, the latter tissue undergoing macrophage infiltration at 4 dpi. BioMed Central 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/41941/1/41941.pdf Rasoli, Mehdi and Yeap, Swee Keong and Tan, Sheau Wei and Roohani, Kiarash and Teo, Kristeen Ye Wen and Mohamed Alitheen, Noorjahan Banu and Abd Rahman, Yasmin and Ideris, Aini and Bejo, Mohd Hair and Kaiser, Pete and Omar, Abdul Rahman (2015) Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus. BMC Veterinary Research, 11. art. no. 75. pp. 1-14. ISSN 1746-6148 http://bmcvetres.biomedcentral.com/articles/10.1186/s12917-015-0377-x 10.1186/s12917-015-0377-x |
| spellingShingle | Rasoli, Mehdi Yeap, Swee Keong Tan, Sheau Wei Roohani, Kiarash Teo, Kristeen Ye Wen Mohamed Alitheen, Noorjahan Banu Abd Rahman, Yasmin Ideris, Aini Bejo, Mohd Hair Kaiser, Pete Omar, Abdul Rahman Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus |
| title | Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus |
| title_full | Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus |
| title_fullStr | Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus |
| title_full_unstemmed | Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus |
| title_short | Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus |
| title_sort | differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus |
| url | http://psasir.upm.edu.my/id/eprint/41941/1/41941.pdf |
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