The recovery of entomopathogenic nematodes from selected areas within Peninsular Malaysia

Soil sampling was conducted within Peninsular Malaysia with the aim of recovering entomopathogenic nematodes (Steinernematidae and Heterorhabditidae). Extensive sampling was performed in the Cameron Highlands, which are climatically distinct from the lowlands, and characterized by lower temperature...

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Main Authors: Mason, J. M., Razak, A. R., Wright, D. J.
Format: Article
Language:English
Published: Cambridge University Press 1996
Online Access:http://psasir.upm.edu.my/id/eprint/42402/1/The%20recovery%20of%20entomopathogenic%20nematodes%20from%20selected%20areas%20within%20Peninsular%20Malaysia.pdf
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author Mason, J. M.
Razak, A. R.
Wright, D. J.
author_facet Mason, J. M.
Razak, A. R.
Wright, D. J.
author_sort Mason, J. M.
collection UPM
description Soil sampling was conducted within Peninsular Malaysia with the aim of recovering entomopathogenic nematodes (Steinernematidae and Heterorhabditidae). Extensive sampling was performed in the Cameron Highlands, which are climatically distinct from the lowlands, and characterized by lower temperature and humidity. The major areas sampled in the lowlands were at the campus of Universiti Pertanian Malaysia (orchards and plantations), Puchong (secondary rainforest) and along the east coast of the country. Entomopathogenic nematodes were recovered using the Galleria mellonella baiting method. Nematodes were recovered from 10% of the 425 samples assayed. Identifications, using a PCR method, revealed that the 21 identified steinernematids belonged to two different genetic types and that four out of the five heterorhabditids were Heterorhabditis indicus, the remaining heterorhabditid being a new species. The nematodes are currently being screened to evaluate their biocontrol potential for use in Malaysia against foliage-feeding lepidopteran pests of crucifers.
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spelling upm.eprints-424022016-04-13T05:17:58Z http://psasir.upm.edu.my/id/eprint/42402/ The recovery of entomopathogenic nematodes from selected areas within Peninsular Malaysia Mason, J. M. Razak, A. R. Wright, D. J. Soil sampling was conducted within Peninsular Malaysia with the aim of recovering entomopathogenic nematodes (Steinernematidae and Heterorhabditidae). Extensive sampling was performed in the Cameron Highlands, which are climatically distinct from the lowlands, and characterized by lower temperature and humidity. The major areas sampled in the lowlands were at the campus of Universiti Pertanian Malaysia (orchards and plantations), Puchong (secondary rainforest) and along the east coast of the country. Entomopathogenic nematodes were recovered using the Galleria mellonella baiting method. Nematodes were recovered from 10% of the 425 samples assayed. Identifications, using a PCR method, revealed that the 21 identified steinernematids belonged to two different genetic types and that four out of the five heterorhabditids were Heterorhabditis indicus, the remaining heterorhabditid being a new species. The nematodes are currently being screened to evaluate their biocontrol potential for use in Malaysia against foliage-feeding lepidopteran pests of crucifers. Cambridge University Press 1996 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/42402/1/The%20recovery%20of%20entomopathogenic%20nematodes%20from%20selected%20areas%20within%20Peninsular%20Malaysia.pdf Mason, J. M. and Razak, A. R. and Wright, D. J. (1996) The recovery of entomopathogenic nematodes from selected areas within Peninsular Malaysia. Journal of Helminthology, 70 (4). pp. 303-307. ISSN 0022-149X; ESSN: 1475-2697 http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1726584 10.1017/S0022149X00015595
spellingShingle Mason, J. M.
Razak, A. R.
Wright, D. J.
The recovery of entomopathogenic nematodes from selected areas within Peninsular Malaysia
title The recovery of entomopathogenic nematodes from selected areas within Peninsular Malaysia
title_full The recovery of entomopathogenic nematodes from selected areas within Peninsular Malaysia
title_fullStr The recovery of entomopathogenic nematodes from selected areas within Peninsular Malaysia
title_full_unstemmed The recovery of entomopathogenic nematodes from selected areas within Peninsular Malaysia
title_short The recovery of entomopathogenic nematodes from selected areas within Peninsular Malaysia
title_sort recovery of entomopathogenic nematodes from selected areas within peninsular malaysia
url http://psasir.upm.edu.my/id/eprint/42402/1/The%20recovery%20of%20entomopathogenic%20nematodes%20from%20selected%20areas%20within%20Peninsular%20Malaysia.pdf
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