In-situ crosslinking of Aspergillus flavus lipase: improvement of activity, stability and properties
The mycelium-bound lipase of Aspergillus flavus was stabilised by cross-linkage using glutaraldehyde or methylglyoxal. Lipase activity was enhanced by up to 48% by treatment with methylglyoxal but not by glutaraldehyde. Cross-linking with methylglyoxal increased the thermal stability of the lipase b...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Kluwer Academic Publishers
1996
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| Online Access: | http://psasir.upm.edu.my/id/eprint/42473/1/In-situ%20crosslinking%20of%20Aspergillus%20flavus%20lipase%20improvement%20of%20activity%2C%20stability%20and%20properties.pdf http://psasir.upm.edu.my/id/eprint/42473/7/BF00128587.pdf |
| _version_ | 1825929170094391296 |
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| author | Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Ampon, Kamaruzaman Bucke, Christopher |
| author_facet | Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Ampon, Kamaruzaman Bucke, Christopher |
| author_sort | Long, Kamariah |
| collection | UPM |
| description | The mycelium-bound lipase of Aspergillus flavus was stabilised by cross-linkage using glutaraldehyde or methylglyoxal. Lipase activity was enhanced by up to 48% by treatment with methylglyoxal but not by glutaraldehyde. Cross-linking with methylglyoxal increased the thermal stability of the lipase by 58% at 50°C. |
| first_indexed | 2024-03-06T08:52:54Z |
| format | Article |
| id | upm.eprints-42473 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-09-25T03:34:27Z |
| publishDate | 1996 |
| publisher | Kluwer Academic Publishers |
| record_format | dspace |
| spelling | upm.eprints-424732024-08-07T07:36:12Z http://psasir.upm.edu.my/id/eprint/42473/ In-situ crosslinking of Aspergillus flavus lipase: improvement of activity, stability and properties Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Ampon, Kamaruzaman Bucke, Christopher The mycelium-bound lipase of Aspergillus flavus was stabilised by cross-linkage using glutaraldehyde or methylglyoxal. Lipase activity was enhanced by up to 48% by treatment with methylglyoxal but not by glutaraldehyde. Cross-linking with methylglyoxal increased the thermal stability of the lipase by 58% at 50°C. Kluwer Academic Publishers 1996 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/42473/1/In-situ%20crosslinking%20of%20Aspergillus%20flavus%20lipase%20improvement%20of%20activity%2C%20stability%20and%20properties.pdf text en http://psasir.upm.edu.my/id/eprint/42473/7/BF00128587.pdf Long, Kamariah and Mohd Ghazali, Hasanah and Ariff, Arbakariya and Ampon, Kamaruzaman and Bucke, Christopher (1996) In-situ crosslinking of Aspergillus flavus lipase: improvement of activity, stability and properties. Biotechnology Letters, 18 (10). pp. 1169-1174. ISSN 0141-5492; ESSN: 1573-6776 http://link.springer.com/article/10.1007%2FBF00128587 10.1007/BF00128587 |
| spellingShingle | Long, Kamariah Mohd Ghazali, Hasanah Ariff, Arbakariya Ampon, Kamaruzaman Bucke, Christopher In-situ crosslinking of Aspergillus flavus lipase: improvement of activity, stability and properties |
| title | In-situ crosslinking of Aspergillus flavus lipase: improvement of activity, stability and properties |
| title_full | In-situ crosslinking of Aspergillus flavus lipase: improvement of activity, stability and properties |
| title_fullStr | In-situ crosslinking of Aspergillus flavus lipase: improvement of activity, stability and properties |
| title_full_unstemmed | In-situ crosslinking of Aspergillus flavus lipase: improvement of activity, stability and properties |
| title_short | In-situ crosslinking of Aspergillus flavus lipase: improvement of activity, stability and properties |
| title_sort | in situ crosslinking of aspergillus flavus lipase improvement of activity stability and properties |
| url | http://psasir.upm.edu.my/id/eprint/42473/1/In-situ%20crosslinking%20of%20Aspergillus%20flavus%20lipase%20improvement%20of%20activity%2C%20stability%20and%20properties.pdf http://psasir.upm.edu.my/id/eprint/42473/7/BF00128587.pdf |
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