Antibiotic resistance evolution of methicillin resistant Staphylococcus aureus (MRSA) and colloidal silver as the nanoweapon
As the society begin to realize the importance of combating antimicrobial resistance, going back to silver might be the solution. Silver has been known for its potential antimicrobial activity since ancient times and, the development of nanoparticles has increased its potential into becoming an anti...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43189/1/%2846%29.pdf |
| _version_ | 1825929311209652224 |
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| author | New, Chia Yeung A. R., Amalia Bahumaish, Ramzi Othman Saeed Radu, Son |
| author_facet | New, Chia Yeung A. R., Amalia Bahumaish, Ramzi Othman Saeed Radu, Son |
| author_sort | New, Chia Yeung |
| collection | UPM |
| description | As the society begin to realize the importance of combating antimicrobial resistance, going back to silver might be the solution. Silver has been known for its potential antimicrobial activity since ancient times and, the development of nanoparticles has increased its potential into becoming an antimicrobial agent that can be applied in broad-spectrum. Antimicrobial resistance has spread into an irrepressible manner which requires drastic action plan as a number of pathogenic bacteria began to acquire resistance genes. Methicillin Resistant Staphylococcus aureus (MRSA) is one of the earliest reported resistant clones which is the center of this study. This study focused on the dissemination and evolution of MRSA on its resistance towards antibiotics. Disc Diffusion Test was employed to create the antibiograms of MRSA isolates. All isolates showed resistance towards amoxicillin, ampicillin, cefazolin, oxacillin and penicillin. In contrast, all isolates were susceptible towards erythromycin. The findings also discovered isolates that were vancomycin-resistant (66.7%) and vancomycin-intermediate (33.3%). As the efficacy of antibiotic treatment is at a question, we also investigated on the antimicrobial activity of colloidal silver in the hope as an alternative treatment. Shiga Toxin producing Escherichia coli (STEC) and MRSA (ATCC 33591) was tested using modified Quantitative suspension test for the evaluation of bactericidal activity for chemical disinfectants and antiseptics based on BS EN 1276:2009. The outcome of this study indicated that the colloidal silver is working effectively against STEC and MRSA (ATCC 33591), showing killing percentages well above 99.0% at 4 minutes and 8 minutes of contact. Vancomycin-resistant S. aureus (VRSA) and Vancomycin-intermediate S. aureus (VISA) were also tested and the results indicated that VISA had higher killing percentages at 4 minutes (99.83%) and 8 minutes (99.85%) compared to VRSA at 4 minutes (96.72%) and 8 minutes (98.35%). This opens a solution to the rising problem of antimicrobial resistance. |
| first_indexed | 2024-03-06T08:54:55Z |
| format | Article |
| id | upm.eprints-43189 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:54:55Z |
| publishDate | 2016 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-431892016-08-03T03:04:08Z http://psasir.upm.edu.my/id/eprint/43189/ Antibiotic resistance evolution of methicillin resistant Staphylococcus aureus (MRSA) and colloidal silver as the nanoweapon New, Chia Yeung A. R., Amalia Bahumaish, Ramzi Othman Saeed Radu, Son As the society begin to realize the importance of combating antimicrobial resistance, going back to silver might be the solution. Silver has been known for its potential antimicrobial activity since ancient times and, the development of nanoparticles has increased its potential into becoming an antimicrobial agent that can be applied in broad-spectrum. Antimicrobial resistance has spread into an irrepressible manner which requires drastic action plan as a number of pathogenic bacteria began to acquire resistance genes. Methicillin Resistant Staphylococcus aureus (MRSA) is one of the earliest reported resistant clones which is the center of this study. This study focused on the dissemination and evolution of MRSA on its resistance towards antibiotics. Disc Diffusion Test was employed to create the antibiograms of MRSA isolates. All isolates showed resistance towards amoxicillin, ampicillin, cefazolin, oxacillin and penicillin. In contrast, all isolates were susceptible towards erythromycin. The findings also discovered isolates that were vancomycin-resistant (66.7%) and vancomycin-intermediate (33.3%). As the efficacy of antibiotic treatment is at a question, we also investigated on the antimicrobial activity of colloidal silver in the hope as an alternative treatment. Shiga Toxin producing Escherichia coli (STEC) and MRSA (ATCC 33591) was tested using modified Quantitative suspension test for the evaluation of bactericidal activity for chemical disinfectants and antiseptics based on BS EN 1276:2009. The outcome of this study indicated that the colloidal silver is working effectively against STEC and MRSA (ATCC 33591), showing killing percentages well above 99.0% at 4 minutes and 8 minutes of contact. Vancomycin-resistant S. aureus (VRSA) and Vancomycin-intermediate S. aureus (VISA) were also tested and the results indicated that VISA had higher killing percentages at 4 minutes (99.83%) and 8 minutes (99.85%) compared to VRSA at 4 minutes (96.72%) and 8 minutes (98.35%). This opens a solution to the rising problem of antimicrobial resistance. Faculty of Food Science and Technology, Universiti Putra Malaysia 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43189/1/%2846%29.pdf New, Chia Yeung and A. R., Amalia and Bahumaish, Ramzi Othman Saeed and Radu, Son (2016) Antibiotic resistance evolution of methicillin resistant Staphylococcus aureus (MRSA) and colloidal silver as the nanoweapon. International Food Research Journal, 23 (3). pp. 1248-1254. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/23%20(03)%202016/(46).pdf |
| spellingShingle | New, Chia Yeung A. R., Amalia Bahumaish, Ramzi Othman Saeed Radu, Son Antibiotic resistance evolution of methicillin resistant Staphylococcus aureus (MRSA) and colloidal silver as the nanoweapon |
| title | Antibiotic resistance evolution of methicillin resistant Staphylococcus aureus (MRSA) and colloidal silver as the nanoweapon |
| title_full | Antibiotic resistance evolution of methicillin resistant Staphylococcus aureus (MRSA) and colloidal silver as the nanoweapon |
| title_fullStr | Antibiotic resistance evolution of methicillin resistant Staphylococcus aureus (MRSA) and colloidal silver as the nanoweapon |
| title_full_unstemmed | Antibiotic resistance evolution of methicillin resistant Staphylococcus aureus (MRSA) and colloidal silver as the nanoweapon |
| title_short | Antibiotic resistance evolution of methicillin resistant Staphylococcus aureus (MRSA) and colloidal silver as the nanoweapon |
| title_sort | antibiotic resistance evolution of methicillin resistant staphylococcus aureus mrsa and colloidal silver as the nanoweapon |
| url | http://psasir.upm.edu.my/id/eprint/43189/1/%2846%29.pdf |
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