Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning
Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (−9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres t...
| Main Authors: | , , , , , |
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| 格式: | 文件 |
| 语言: | English |
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Elsevier
2016
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| 在线阅读: | http://psasir.upm.edu.my/id/eprint/43211/1/Immobilisation%20of%20cyclodextrin%20glucanotransferase%20into%20polyvinyl%20alcohol%20%28PVA%29%20nanofibres%20via%20electrospinning.pdf |
| _version_ | 1825929315786686464 |
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| author | Saallah, Suryani Naim, Mohd Nazli Lenggoro, Ignatius Wuled Mokhtar, Mohd Noriznan Abu Bakar, Noor Fitrah Gen, Masao |
| author_facet | Saallah, Suryani Naim, Mohd Nazli Lenggoro, Ignatius Wuled Mokhtar, Mohd Noriznan Abu Bakar, Noor Fitrah Gen, Masao |
| author_sort | Saallah, Suryani |
| collection | UPM |
| description | Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (−9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film. |
| first_indexed | 2024-03-06T08:54:59Z |
| format | Article |
| id | upm.eprints-43211 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:54:59Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-432112016-05-18T04:11:45Z http://psasir.upm.edu.my/id/eprint/43211/ Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning Saallah, Suryani Naim, Mohd Nazli Lenggoro, Ignatius Wuled Mokhtar, Mohd Noriznan Abu Bakar, Noor Fitrah Gen, Masao Immobilisation of cyclodextrin glucanotransferase (CGTase) on nanofibres was demonstrated. CGTase solution (1% v/v) and PVA (8 wt%) solution were mixed followed by electrospinning (−9 kV, 3 h). CGTase/PVA nanofibres with an average diameter of 176 ± 46 nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. A CGTase/PVA film made up from the same mixture and treated the same way was used as a control experiment. The immobilised CGTase on nanofibres showed superior performance with nearly a 2.5 fold higher enzyme loading and 31% higher enzyme activity in comparison with the film. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43211/1/Immobilisation%20of%20cyclodextrin%20glucanotransferase%20into%20polyvinyl%20alcohol%20%28PVA%29%20nanofibres%20via%20electrospinning.pdf Saallah, Suryani and Naim, Mohd Nazli and Lenggoro, Ignatius Wuled and Mokhtar, Mohd Noriznan and Abu Bakar, Noor Fitrah and Gen, Masao (2016) Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning. Biotechnology Reports, 10. pp. 44-48. ISSN 2215-017X http://www.sciencedirect.com/science/article/pii/S2215017X16300108 10.1016/j.btre.2016.03.003 |
| spellingShingle | Saallah, Suryani Naim, Mohd Nazli Lenggoro, Ignatius Wuled Mokhtar, Mohd Noriznan Abu Bakar, Noor Fitrah Gen, Masao Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning |
| title | Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning |
| title_full | Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning |
| title_fullStr | Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning |
| title_full_unstemmed | Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning |
| title_short | Immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol (PVA) nanofibres via electrospinning |
| title_sort | immobilisation of cyclodextrin glucanotransferase into polyvinyl alcohol pva nanofibres via electrospinning |
| url | http://psasir.upm.edu.my/id/eprint/43211/1/Immobilisation%20of%20cyclodextrin%20glucanotransferase%20into%20polyvinyl%20alcohol%20%28PVA%29%20nanofibres%20via%20electrospinning.pdf |
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