A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes
A novel optical detection system consisting of combination of uricase/HRP–CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horse...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43602/1/A%20simple%20and%20sensitive%20fluorescence%20based%20biosensor%20for%20the%20determination%20of%20uric%20acid%20.pdf |
| _version_ | 1796974375829766144 |
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| author | Azmi, Nur Ellina Ramli, Noor Izaanin Abdullah, Jaafar Abdul Hamid, Mohammad Azmi Sidek, Hamidah Abd Rahman, Samsulida Ariffin, Nurhayati Yusof, Nor Azah |
| author_facet | Azmi, Nur Ellina Ramli, Noor Izaanin Abdullah, Jaafar Abdul Hamid, Mohammad Azmi Sidek, Hamidah Abd Rahman, Samsulida Ariffin, Nurhayati Yusof, Nor Azah |
| author_sort | Azmi, Nur Ellina |
| collection | UPM |
| description | A novel optical detection system consisting of combination of uricase/HRP–CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125–1000 µM with detection limit of 125 µM. |
| first_indexed | 2024-03-06T08:56:09Z |
| format | Article |
| id | upm.eprints-43602 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:56:09Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-436022017-11-01T09:18:11Z http://psasir.upm.edu.my/id/eprint/43602/ A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes Azmi, Nur Ellina Ramli, Noor Izaanin Abdullah, Jaafar Abdul Hamid, Mohammad Azmi Sidek, Hamidah Abd Rahman, Samsulida Ariffin, Nurhayati Yusof, Nor Azah A novel optical detection system consisting of combination of uricase/HRP–CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125–1000 µM with detection limit of 125 µM. Elsevier 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43602/1/A%20simple%20and%20sensitive%20fluorescence%20based%20biosensor%20for%20the%20determination%20of%20uric%20acid%20.pdf Azmi, Nur Ellina and Ramli, Noor Izaanin and Abdullah, Jaafar and Abdul Hamid, Mohammad Azmi and Sidek, Hamidah and Abd Rahman, Samsulida and Ariffin, Nurhayati and Yusof, Nor Azah (2015) A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes. Biosensors and Bioelectronics, 61. pp. 129-133. ISSN 0956-5663; ESSN: 1873-4235 http://www.sciencedirect.com/science/article/pii/S0956566314005570 10.1016/j.bios.2014.07.056 |
| spellingShingle | Azmi, Nur Ellina Ramli, Noor Izaanin Abdullah, Jaafar Abdul Hamid, Mohammad Azmi Sidek, Hamidah Abd Rahman, Samsulida Ariffin, Nurhayati Yusof, Nor Azah A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes |
| title | A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes |
| title_full | A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes |
| title_fullStr | A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes |
| title_full_unstemmed | A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes |
| title_short | A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes |
| title_sort | simple and sensitive fluorescence based biosensor for the determination of uric acid using h2o2 sensitive quantum dots dual enzymes |
| url | http://psasir.upm.edu.my/id/eprint/43602/1/A%20simple%20and%20sensitive%20fluorescence%20based%20biosensor%20for%20the%20determination%20of%20uric%20acid%20.pdf |
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