An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies
Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid. Parameters assessed included the effect of PVS2...
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| Format: | Article |
| Language: | English |
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Scientific and Technological Research Council of Turkey
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43724/1/An%20improved%20PVS2%20cryopreservation%20technique%20for%20Ascocenda%20.pdf |
| _version_ | 1796974400694648832 |
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| author | Rajasegar, Arulvilee Mansor, Asyraf Poobathy, Ranjetta Sivalingam, Elayabalan Sinniah, Uma Rani Subramaniam, Sreeramanan |
| author_facet | Rajasegar, Arulvilee Mansor, Asyraf Poobathy, Ranjetta Sivalingam, Elayabalan Sinniah, Uma Rani Subramaniam, Sreeramanan |
| author_sort | Rajasegar, Arulvilee |
| collection | UPM |
| description | Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid. Parameters assessed included the effect of PVS2 exposure periods, thawing duration, temperature, and culture conditions based on 2,3,5-triphenyltetrazolium chloride absorbance readings and regrowth rates. A regrowth rate of 33.3% was obtained after 2 months when the PLBs were dehydrated in PVS2 for 30 min. The growth rate was improved to 47% when thawing was conducted at 45 °C for 85 s. The highest growth rate (53.3%) was obtained when the PLBs were subjected to a 7-day dark treatment before being transferred to a 16-h/8-h light/dark photoperiod. Histological analyses were conducted to study the morphology of cryopreserved and noncryopreserved PLBs of Ascocenda Wangsa Gold. The vitrification protocol developed in this study is a feasible and safe method for strengthening the germplasm conservation of this orchid for commercial purposes. |
| first_indexed | 2024-03-06T08:56:31Z |
| format | Article |
| id | upm.eprints-43724 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:56:31Z |
| publishDate | 2015 |
| publisher | Scientific and Technological Research Council of Turkey |
| record_format | dspace |
| spelling | upm.eprints-437242016-08-09T09:36:18Z http://psasir.upm.edu.my/id/eprint/43724/ An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies Rajasegar, Arulvilee Mansor, Asyraf Poobathy, Ranjetta Sivalingam, Elayabalan Sinniah, Uma Rani Subramaniam, Sreeramanan Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid. Parameters assessed included the effect of PVS2 exposure periods, thawing duration, temperature, and culture conditions based on 2,3,5-triphenyltetrazolium chloride absorbance readings and regrowth rates. A regrowth rate of 33.3% was obtained after 2 months when the PLBs were dehydrated in PVS2 for 30 min. The growth rate was improved to 47% when thawing was conducted at 45 °C for 85 s. The highest growth rate (53.3%) was obtained when the PLBs were subjected to a 7-day dark treatment before being transferred to a 16-h/8-h light/dark photoperiod. Histological analyses were conducted to study the morphology of cryopreserved and noncryopreserved PLBs of Ascocenda Wangsa Gold. The vitrification protocol developed in this study is a feasible and safe method for strengthening the germplasm conservation of this orchid for commercial purposes. Scientific and Technological Research Council of Turkey 2015-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43724/1/An%20improved%20PVS2%20cryopreservation%20technique%20for%20Ascocenda%20.pdf Rajasegar, Arulvilee and Mansor, Asyraf and Poobathy, Ranjetta and Sivalingam, Elayabalan and Sinniah, Uma Rani and Subramaniam, Sreeramanan (2015) An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies. Turkish Journal of Biology, 39. pp. 202-209. ISSN 1300-0152; ESSN: 1303-6092 http://journals.tubitak.gov.tr/biology/issues/biy-15-39-2/biy-39-2-3-1405-7.pdf 10.3906/biy-1405-7 |
| spellingShingle | Rajasegar, Arulvilee Mansor, Asyraf Poobathy, Ranjetta Sivalingam, Elayabalan Sinniah, Uma Rani Subramaniam, Sreeramanan An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies |
| title | An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies |
| title_full | An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies |
| title_fullStr | An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies |
| title_full_unstemmed | An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies |
| title_short | An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies |
| title_sort | improved pvs2 cryopreservation technique for ascocenda wangsa gold orchid using protocorm like bodies |
| url | http://psasir.upm.edu.my/id/eprint/43724/1/An%20improved%20PVS2%20cryopreservation%20technique%20for%20Ascocenda%20.pdf |
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