An investigation into the antiobesity effects of Morinda citrifolia L. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach
The prevalence of obesity is increasing worldwide, with high fat diet (HFD) as one of the main contributing factors. Obesity increases the predisposition to other diseases such as diabetes through various metabolic pathways. Limited availability of antiobesity drugs and the popularity of complementa...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/43729/1/43729.pdf |
| _version_ | 1796974401714913280 |
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| author | Jambocus, Najla Gooda Sahib Saari, Nazamid Ismail, Amin Khatib, Alfi Mahomoodally, Mohamad Fawzi Abdul Hamid, Azizah |
| author_facet | Jambocus, Najla Gooda Sahib Saari, Nazamid Ismail, Amin Khatib, Alfi Mahomoodally, Mohamad Fawzi Abdul Hamid, Azizah |
| author_sort | Jambocus, Najla Gooda Sahib |
| collection | UPM |
| description | The prevalence of obesity is increasing worldwide, with high fat diet (HFD) as one of the main contributing factors. Obesity increases the predisposition to other diseases such as diabetes through various metabolic pathways. Limited availability of antiobesity drugs and the popularity of complementary medicine have encouraged research in finding phytochemical strategies to this multifaceted disease. HFD induced obese Sprague-Dawley rats were treated with an extract of Morinda citrifolia L. leaves (MLE 60). After 9 weeks of treatment, positive effects were observed on adiposity, fecal fat content, plasma lipids, and insulin and leptin levels. The inducement of obesity and treatment with MLE 60 on metabolic alterations were then further elucidated using a 1H NMR based metabolomics approach. Discriminating metabolites involved were products of various metabolic pathways, including glucose metabolism and TCA cycle (lactate, 2-oxoglutarate, citrate, succinate, pyruvate, and acetate), amino acid metabolism (alanine, 2-hydroxybutyrate), choline metabolism (betaine), creatinine metabolism (creatinine), and gut microbiome metabolism (hippurate, phenylacetylglycine, dimethylamine, and trigonelline). Treatment with MLE 60 resulted in significant improvement in the metabolic perturbations caused obesity as demonstrated by the proximity of the treated group to the normal group in the OPLS-DA score plot and the change in trajectory movement of the diseased group towards the healthy group upon treatment. |
| first_indexed | 2024-03-06T08:56:32Z |
| format | Article |
| id | upm.eprints-43729 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:56:32Z |
| publishDate | 2016 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-437292018-10-09T02:40:28Z http://psasir.upm.edu.my/id/eprint/43729/ An investigation into the antiobesity effects of Morinda citrifolia L. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach Jambocus, Najla Gooda Sahib Saari, Nazamid Ismail, Amin Khatib, Alfi Mahomoodally, Mohamad Fawzi Abdul Hamid, Azizah The prevalence of obesity is increasing worldwide, with high fat diet (HFD) as one of the main contributing factors. Obesity increases the predisposition to other diseases such as diabetes through various metabolic pathways. Limited availability of antiobesity drugs and the popularity of complementary medicine have encouraged research in finding phytochemical strategies to this multifaceted disease. HFD induced obese Sprague-Dawley rats were treated with an extract of Morinda citrifolia L. leaves (MLE 60). After 9 weeks of treatment, positive effects were observed on adiposity, fecal fat content, plasma lipids, and insulin and leptin levels. The inducement of obesity and treatment with MLE 60 on metabolic alterations were then further elucidated using a 1H NMR based metabolomics approach. Discriminating metabolites involved were products of various metabolic pathways, including glucose metabolism and TCA cycle (lactate, 2-oxoglutarate, citrate, succinate, pyruvate, and acetate), amino acid metabolism (alanine, 2-hydroxybutyrate), choline metabolism (betaine), creatinine metabolism (creatinine), and gut microbiome metabolism (hippurate, phenylacetylglycine, dimethylamine, and trigonelline). Treatment with MLE 60 resulted in significant improvement in the metabolic perturbations caused obesity as demonstrated by the proximity of the treated group to the normal group in the OPLS-DA score plot and the change in trajectory movement of the diseased group towards the healthy group upon treatment. Hindawi Publishing Corporation 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/43729/1/43729.pdf Jambocus, Najla Gooda Sahib and Saari, Nazamid and Ismail, Amin and Khatib, Alfi and Mahomoodally, Mohamad Fawzi and Abdul Hamid, Azizah (2016) An investigation into the antiobesity effects of Morinda citrifolia L. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach. Journal of Diabetes Research, 2016. art. no. 2391592. pp. 1-14. ISSN 2314-6745; ESSN: 2314-6753 https://www.hindawi.com/journals/jdr/2016/2391592/abs/ 10.1155/2016/2391592 |
| spellingShingle | Jambocus, Najla Gooda Sahib Saari, Nazamid Ismail, Amin Khatib, Alfi Mahomoodally, Mohamad Fawzi Abdul Hamid, Azizah An investigation into the antiobesity effects of Morinda citrifolia L. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach |
| title | An investigation into the antiobesity effects of Morinda citrifolia L. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach |
| title_full | An investigation into the antiobesity effects of Morinda citrifolia L. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach |
| title_fullStr | An investigation into the antiobesity effects of Morinda citrifolia L. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach |
| title_full_unstemmed | An investigation into the antiobesity effects of Morinda citrifolia L. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach |
| title_short | An investigation into the antiobesity effects of Morinda citrifolia L. leaf extract in high fat diet induced obese rats using a 1H NMR metabolomics approach |
| title_sort | investigation into the antiobesity effects of morinda citrifolia l leaf extract in high fat diet induced obese rats using a 1h nmr metabolomics approach |
| url | http://psasir.upm.edu.my/id/eprint/43729/1/43729.pdf |
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