Application of response surface methodology for optimising caffeine-degrading parameters by Leifsonia sp. strain SIU
Caffeine is an important naturally occurring compound which can be degraded by bacteria. Previously, Leifsonia sp. strain SIU capable of degrading caffeine was isolated from agricultural soil. Plackett-Burman design was used to screen significant parameters that affect the rate of caffeine degradati...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Triveni Enterprises, Lucknow
2015
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/43820/1/Application%20of%20response%20surface%20methodology%20for%20optimising%20caffeine-degrading%20.pdf |
| _version_ | 1796974420104839168 |
|---|---|
| author | Ibrahim, Salihu Shukor, Mohd Yunus Abdul Khalil, Khalilah Effendi Halmi, Mohd Izuan Syed, Mohd. Arif Ahmad, Siti Aqlima |
| author_facet | Ibrahim, Salihu Shukor, Mohd Yunus Abdul Khalil, Khalilah Effendi Halmi, Mohd Izuan Syed, Mohd. Arif Ahmad, Siti Aqlima |
| author_sort | Ibrahim, Salihu |
| collection | UPM |
| description | Caffeine is an important naturally occurring compound which can be degraded by bacteria. Previously, Leifsonia sp. strain SIU capable of degrading caffeine was isolated from agricultural soil. Plackett-Burman design was used to screen significant parameters that affect the rate of caffeine degradation. After the design was applied, response surface methodology (RSM) through Central Composite Design (CCD) was used to study significant parameters further, in order to get the most superior degradation conditions. The optimum concentrations of carbon source (sucrose), nitrogen source (NH4Cl), pH and initial caffeine concentration was found to be 5.0 gl(-1), 0.4 gl(-1), 6.0 and 375 ppm respectively. Second order polynomial regression model accurately showed interpretation of experimental data with an R2 value of 0.9989, Adjusted (Adj) R2, Predicted (Pred) R2 and F values of 0.9939, 0.9225 and 88.77 respectively. |
| first_indexed | 2024-03-06T08:56:48Z |
| format | Article |
| id | upm.eprints-43820 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:56:48Z |
| publishDate | 2015 |
| publisher | Triveni Enterprises, Lucknow |
| record_format | dspace |
| spelling | upm.eprints-438202016-09-21T05:13:01Z http://psasir.upm.edu.my/id/eprint/43820/ Application of response surface methodology for optimising caffeine-degrading parameters by Leifsonia sp. strain SIU Ibrahim, Salihu Shukor, Mohd Yunus Abdul Khalil, Khalilah Effendi Halmi, Mohd Izuan Syed, Mohd. Arif Ahmad, Siti Aqlima Caffeine is an important naturally occurring compound which can be degraded by bacteria. Previously, Leifsonia sp. strain SIU capable of degrading caffeine was isolated from agricultural soil. Plackett-Burman design was used to screen significant parameters that affect the rate of caffeine degradation. After the design was applied, response surface methodology (RSM) through Central Composite Design (CCD) was used to study significant parameters further, in order to get the most superior degradation conditions. The optimum concentrations of carbon source (sucrose), nitrogen source (NH4Cl), pH and initial caffeine concentration was found to be 5.0 gl(-1), 0.4 gl(-1), 6.0 and 375 ppm respectively. Second order polynomial regression model accurately showed interpretation of experimental data with an R2 value of 0.9989, Adjusted (Adj) R2, Predicted (Pred) R2 and F values of 0.9939, 0.9225 and 88.77 respectively. Triveni Enterprises, Lucknow 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/43820/1/Application%20of%20response%20surface%20methodology%20for%20optimising%20caffeine-degrading%20.pdf Ibrahim, Salihu and Shukor, Mohd Yunus and Abdul Khalil, Khalilah and Effendi Halmi, Mohd Izuan and Syed, Mohd. Arif and Ahmad, Siti Aqlima (2015) Application of response surface methodology for optimising caffeine-degrading parameters by Leifsonia sp. strain SIU. Journal of Environmental Biology, 36 (5). pp. 1215-1221. ISSN 0254-8704 |
| spellingShingle | Ibrahim, Salihu Shukor, Mohd Yunus Abdul Khalil, Khalilah Effendi Halmi, Mohd Izuan Syed, Mohd. Arif Ahmad, Siti Aqlima Application of response surface methodology for optimising caffeine-degrading parameters by Leifsonia sp. strain SIU |
| title | Application of response surface methodology for optimising caffeine-degrading parameters by Leifsonia sp. strain SIU |
| title_full | Application of response surface methodology for optimising caffeine-degrading parameters by Leifsonia sp. strain SIU |
| title_fullStr | Application of response surface methodology for optimising caffeine-degrading parameters by Leifsonia sp. strain SIU |
| title_full_unstemmed | Application of response surface methodology for optimising caffeine-degrading parameters by Leifsonia sp. strain SIU |
| title_short | Application of response surface methodology for optimising caffeine-degrading parameters by Leifsonia sp. strain SIU |
| title_sort | application of response surface methodology for optimising caffeine degrading parameters by leifsonia sp strain siu |
| url | http://psasir.upm.edu.my/id/eprint/43820/1/Application%20of%20response%20surface%20methodology%20for%20optimising%20caffeine-degrading%20.pdf |
| work_keys_str_mv | AT ibrahimsalihu applicationofresponsesurfacemethodologyforoptimisingcaffeinedegradingparametersbyleifsoniaspstrainsiu AT shukormohdyunus applicationofresponsesurfacemethodologyforoptimisingcaffeinedegradingparametersbyleifsoniaspstrainsiu AT abdulkhalilkhalilah applicationofresponsesurfacemethodologyforoptimisingcaffeinedegradingparametersbyleifsoniaspstrainsiu AT effendihalmimohdizuan applicationofresponsesurfacemethodologyforoptimisingcaffeinedegradingparametersbyleifsoniaspstrainsiu AT syedmohdarif applicationofresponsesurfacemethodologyforoptimisingcaffeinedegradingparametersbyleifsoniaspstrainsiu AT ahmadsitiaqlima applicationofresponsesurfacemethodologyforoptimisingcaffeinedegradingparametersbyleifsoniaspstrainsiu |