Spectroscopic identification of geometrical isomers of α- and β-carotenes from palm oil

To date, all identification of palm carotenoids are tentative based on electronic absorption spectra, comparison of the elution sequence with past studies and authentic standards. This study reports the isolation of individual major isomers of palm carotenes using a semi-preparative C30 column. The...

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Bibliographic Details
Main Authors: Tay, Bonnie Yen Ping, Ee, Gwendoline Cheng Lian
Format: Article
Published: Malaysian Palm Oil Board 2005
Description
Summary:To date, all identification of palm carotenoids are tentative based on electronic absorption spectra, comparison of the elution sequence with past studies and authentic standards. This study reports the isolation of individual major isomers of palm carotenes using a semi-preparative C30 column. The results of MS, 1 H NMR of four isolated peaks, assigned as Fraction 1 (a mixture of 13 and 13’ cis α-carotene), Fraction 2 ( 13 cis β-carotene), Fraction 3 (all trans α-carotene) and Fraction 4 (cis β-carotene) supported the identification of their structures.