Cytotoxicity and physicochemical characterization of iron–manganese-doped sulfated zirconia nanoparticles
Iron–manganese-doped sulfated zirconia nanoparticles with both Lewis and Brønsted acidic sites were prepared by a hydrothermal impregnation method followed by calcination at 650°C for 5 hours, and their cytotoxicity properties against cancer cell lines were determined. The characterization was carri...
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| Format: | Article |
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Dove Medical Press
2015
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| author | Al-Fahdawi, Mohamed Qasim Abdullah, Rasedee Al-Qubaisi, Mothanna Sadiq Alhassan, Fatah H. Rosli, Rozita El Zowalaty, Mohamed Ezzat Naadja, Seïf-Eddine Webster, Thomas J. Taufiq-Yap, Yun Hin |
| author_facet | Al-Fahdawi, Mohamed Qasim Abdullah, Rasedee Al-Qubaisi, Mothanna Sadiq Alhassan, Fatah H. Rosli, Rozita El Zowalaty, Mohamed Ezzat Naadja, Seïf-Eddine Webster, Thomas J. Taufiq-Yap, Yun Hin |
| author_sort | Al-Fahdawi, Mohamed Qasim |
| collection | UPM |
| description | Iron–manganese-doped sulfated zirconia nanoparticles with both Lewis and Brønsted acidic sites were prepared by a hydrothermal impregnation method followed by calcination at 650°C for 5 hours, and their cytotoxicity properties against cancer cell lines were determined. The characterization was carried out using X-ray diffraction, thermogravimetric analysis, Fourier transform infrared spectroscopy, Brauner–Emmett–Teller (BET) surface area measurements, X-ray fluorescence, X-ray photoelectron spectroscopy, zeta size potential, and transmission electron microscopy (TEM). The cytotoxicity of iron–manganese-doped sulfated zirconia nanoparticles was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays against three human cancer cell lines (breast cancer MDA-MB231 cells, colon carcinoma HT29 cells, and hepatocellular carcinoma HepG2 cells) and two normal human cell lines (normal hepatocyte Chang cells and normal human umbilical vein endothelial cells [HUVECs]). The results suggest for the first time that iron–manganese-doped sulfated zirconia nanoparticles are cytotoxic to MDA-MB231 and HepG2 cancer cells but have less toxicity to HT29 and normal cells at concentrations from 7.8 µg/mL to 500 µg/mL. The morphology of the treated cells was also studied, and the results supported those from the cytotoxicity study in that the nanoparticle-treated HepG2 and MDA-MB231 cells had more dramatic changes in cell morphology than the HT29 cells. In this manner, this study provides the first evidence that iron–manganese-doped sulfated zirconia nanoparticles should be further studied for a wide range of cancer applications without detrimental effects on healthy cell functions. |
| first_indexed | 2024-03-06T08:57:47Z |
| format | Article |
| id | upm.eprints-44232 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T08:57:47Z |
| publishDate | 2015 |
| publisher | Dove Medical Press |
| record_format | dspace |
| spelling | upm.eprints-442322023-12-24T15:52:30Z http://psasir.upm.edu.my/id/eprint/44232/ Cytotoxicity and physicochemical characterization of iron–manganese-doped sulfated zirconia nanoparticles Al-Fahdawi, Mohamed Qasim Abdullah, Rasedee Al-Qubaisi, Mothanna Sadiq Alhassan, Fatah H. Rosli, Rozita El Zowalaty, Mohamed Ezzat Naadja, Seïf-Eddine Webster, Thomas J. Taufiq-Yap, Yun Hin Iron–manganese-doped sulfated zirconia nanoparticles with both Lewis and Brønsted acidic sites were prepared by a hydrothermal impregnation method followed by calcination at 650°C for 5 hours, and their cytotoxicity properties against cancer cell lines were determined. The characterization was carried out using X-ray diffraction, thermogravimetric analysis, Fourier transform infrared spectroscopy, Brauner–Emmett–Teller (BET) surface area measurements, X-ray fluorescence, X-ray photoelectron spectroscopy, zeta size potential, and transmission electron microscopy (TEM). The cytotoxicity of iron–manganese-doped sulfated zirconia nanoparticles was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays against three human cancer cell lines (breast cancer MDA-MB231 cells, colon carcinoma HT29 cells, and hepatocellular carcinoma HepG2 cells) and two normal human cell lines (normal hepatocyte Chang cells and normal human umbilical vein endothelial cells [HUVECs]). The results suggest for the first time that iron–manganese-doped sulfated zirconia nanoparticles are cytotoxic to MDA-MB231 and HepG2 cancer cells but have less toxicity to HT29 and normal cells at concentrations from 7.8 µg/mL to 500 µg/mL. The morphology of the treated cells was also studied, and the results supported those from the cytotoxicity study in that the nanoparticle-treated HepG2 and MDA-MB231 cells had more dramatic changes in cell morphology than the HT29 cells. In this manner, this study provides the first evidence that iron–manganese-doped sulfated zirconia nanoparticles should be further studied for a wide range of cancer applications without detrimental effects on healthy cell functions. Dove Medical Press 2015 Article PeerReviewed Al-Fahdawi, Mohamed Qasim and Abdullah, Rasedee and Al-Qubaisi, Mothanna Sadiq and Alhassan, Fatah H. and Rosli, Rozita and El Zowalaty, Mohamed Ezzat and Naadja, Seïf-Eddine and Webster, Thomas J. and Taufiq-Yap, Yun Hin (2015) Cytotoxicity and physicochemical characterization of iron–manganese-doped sulfated zirconia nanoparticles. International Journal of Nanomedicine, 10 (1). pp. 5739-5750. ISSN 1178-2013 https://www.dovepress.com/cytotoxicity-and-physicochemical-characterization-of-ironndashmanganes-peer-reviewed-fulltext-article-IJN 10.2147/IJN.S82586 |
| spellingShingle | Al-Fahdawi, Mohamed Qasim Abdullah, Rasedee Al-Qubaisi, Mothanna Sadiq Alhassan, Fatah H. Rosli, Rozita El Zowalaty, Mohamed Ezzat Naadja, Seïf-Eddine Webster, Thomas J. Taufiq-Yap, Yun Hin Cytotoxicity and physicochemical characterization of iron–manganese-doped sulfated zirconia nanoparticles |
| title | Cytotoxicity and physicochemical characterization of iron–manganese-doped sulfated zirconia nanoparticles |
| title_full | Cytotoxicity and physicochemical characterization of iron–manganese-doped sulfated zirconia nanoparticles |
| title_fullStr | Cytotoxicity and physicochemical characterization of iron–manganese-doped sulfated zirconia nanoparticles |
| title_full_unstemmed | Cytotoxicity and physicochemical characterization of iron–manganese-doped sulfated zirconia nanoparticles |
| title_short | Cytotoxicity and physicochemical characterization of iron–manganese-doped sulfated zirconia nanoparticles |
| title_sort | cytotoxicity and physicochemical characterization of iron manganese doped sulfated zirconia nanoparticles |
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