miR-205 in situ expression and localization in head and neck tumors - a tissue array study
Background: microRNAs are small non-coding RNA that control gene expression by mRNA degradation or translational inhibition. These molecules are known to play essential roles in many biological and physiological processes. miR-205 may be differentially expressed in head and neck cancers; however, th...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Asian Pacific Organization for Cancer Prevention
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/45489/1/TUMOR.pdf |
| _version_ | 1825929575367966720 |
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| author | Cheah, Yoke Kqueen Ab Mutalib, Nurul Syakima Lee, Learn Han |
| author_facet | Cheah, Yoke Kqueen Ab Mutalib, Nurul Syakima Lee, Learn Han |
| author_sort | Cheah, Yoke Kqueen |
| collection | UPM |
| description | Background: microRNAs are small non-coding RNA that control gene expression by mRNA degradation or translational inhibition. These molecules are known to play essential roles in many biological and physiological processes. miR-205 may be differentially expressed in head and neck cancers; however, there are conflicting data and localization of expression has yet to be determined. Materials and Methods: miR-205 expression was investigated in 48 cases of inflammatory, benign and malignant tumor tissue array of the neck, oronasopharynx,larynx and salivary glands by Locked Nucleic Acid in situ hybridization (LNA-ISH) technology. Results: miR-205 expression was significantly differentially expressed across all of the inflammatory, benign and malignant tumor tissues of the neck. A significant increase in miR-205 staining intensity (p<0.05) was observed from inflammation to benign and malignant tumors in head and neck tissue array, suggesting that miR-205 could be a biomarker to differentiate between cancer and non-cancer tissues. Conclusions: LNA-ISH revealed that miR-205 exhibited significant differential cytoplasmic and nuclear staining among inflammation, benign and malignant tumors of head and neck. miR-205 was not only exclusively expressed in squamous epithelial malignancy. This study offers information and a basis for a comprehensive study of the role of miR-205 that may be useful as a biomarker and/or therapeutic target in head and neck tumors. |
| first_indexed | 2024-03-06T08:58:44Z |
| format | Article |
| id | upm.eprints-45489 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:58:44Z |
| publishDate | 2015 |
| publisher | Asian Pacific Organization for Cancer Prevention |
| record_format | dspace |
| spelling | upm.eprints-454892021-01-23T22:27:33Z http://psasir.upm.edu.my/id/eprint/45489/ miR-205 in situ expression and localization in head and neck tumors - a tissue array study Cheah, Yoke Kqueen Ab Mutalib, Nurul Syakima Lee, Learn Han Background: microRNAs are small non-coding RNA that control gene expression by mRNA degradation or translational inhibition. These molecules are known to play essential roles in many biological and physiological processes. miR-205 may be differentially expressed in head and neck cancers; however, there are conflicting data and localization of expression has yet to be determined. Materials and Methods: miR-205 expression was investigated in 48 cases of inflammatory, benign and malignant tumor tissue array of the neck, oronasopharynx,larynx and salivary glands by Locked Nucleic Acid in situ hybridization (LNA-ISH) technology. Results: miR-205 expression was significantly differentially expressed across all of the inflammatory, benign and malignant tumor tissues of the neck. A significant increase in miR-205 staining intensity (p<0.05) was observed from inflammation to benign and malignant tumors in head and neck tissue array, suggesting that miR-205 could be a biomarker to differentiate between cancer and non-cancer tissues. Conclusions: LNA-ISH revealed that miR-205 exhibited significant differential cytoplasmic and nuclear staining among inflammation, benign and malignant tumors of head and neck. miR-205 was not only exclusively expressed in squamous epithelial malignancy. This study offers information and a basis for a comprehensive study of the role of miR-205 that may be useful as a biomarker and/or therapeutic target in head and neck tumors. Asian Pacific Organization for Cancer Prevention 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45489/1/TUMOR.pdf Cheah, Yoke Kqueen and Ab Mutalib, Nurul Syakima and Lee, Learn Han (2015) miR-205 in situ expression and localization in head and neck tumors - a tissue array study. Asian Pacific journal of cancer prevention, 15 (21). pp. 9071-9075. ISSN 1513-7368 https://pubmed.ncbi.nlm.nih.gov/25422181/ 10.7314/apjcp.2014.15.21.9071 |
| spellingShingle | Cheah, Yoke Kqueen Ab Mutalib, Nurul Syakima Lee, Learn Han miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
| title | miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
| title_full | miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
| title_fullStr | miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
| title_full_unstemmed | miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
| title_short | miR-205 in situ expression and localization in head and neck tumors - a tissue array study |
| title_sort | mir 205 in situ expression and localization in head and neck tumors a tissue array study |
| url | http://psasir.upm.edu.my/id/eprint/45489/1/TUMOR.pdf |
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