Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia
A total of 44 Rhipicephalus sanguineus ticks collected from 23 dogs from Malaysia were screened for Rickettsia, Anaplasmataceae and Coxiella burnetii. Coxiella burnetii was detected in 59% (26/44) of ticks however Rickettsia and Anaplasmataceae were not detected in any of the ticks. In order to geno...
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| Format: | Article |
| Language: | English |
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Malaysian Society of Parasitology and Tropical Medicine
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/45531/1/Molecular%20screening%20for%20Rickettsia%2C%20Anaplasmataceae%20and%20Coxiella%20burnetii%20in%20Rhipicephalus%20sanguineus%20ticks%20from%20Malaysia.pdf |
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| author | Watanabe, Malaika Nakao, R. Babjee, Shaik Mohamed Amin Moktar, Maizatul Akmal Youn, J. H. Qiu, Y. Sugimoto, C. |
| author_facet | Watanabe, Malaika Nakao, R. Babjee, Shaik Mohamed Amin Moktar, Maizatul Akmal Youn, J. H. Qiu, Y. Sugimoto, C. |
| author_sort | Watanabe, Malaika |
| collection | UPM |
| description | A total of 44 Rhipicephalus sanguineus ticks collected from 23 dogs from Malaysia were screened for Rickettsia, Anaplasmataceae and Coxiella burnetii. Coxiella burnetii was detected in 59% (26/44) of ticks however Rickettsia and Anaplasmataceae were not detected in any of the ticks. In order to genotype the strains of C. burnetii, multispacer sequence typing (MST) was carried out using three different spacers. One of the spacers; Cox2 successfully amplified a fragment for which the full length sequence of 397 bp was obtained. The sequenced product revealed only a single nucleotide difference with the Cox2.3 type sequence. |
| first_indexed | 2024-03-06T08:58:47Z |
| format | Article |
| id | upm.eprints-45531 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:58:47Z |
| publishDate | 2015 |
| publisher | Malaysian Society of Parasitology and Tropical Medicine |
| record_format | dspace |
| spelling | upm.eprints-455312021-04-15T02:57:00Z http://psasir.upm.edu.my/id/eprint/45531/ Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia Watanabe, Malaika Nakao, R. Babjee, Shaik Mohamed Amin Moktar, Maizatul Akmal Youn, J. H. Qiu, Y. Sugimoto, C. A total of 44 Rhipicephalus sanguineus ticks collected from 23 dogs from Malaysia were screened for Rickettsia, Anaplasmataceae and Coxiella burnetii. Coxiella burnetii was detected in 59% (26/44) of ticks however Rickettsia and Anaplasmataceae were not detected in any of the ticks. In order to genotype the strains of C. burnetii, multispacer sequence typing (MST) was carried out using three different spacers. One of the spacers; Cox2 successfully amplified a fragment for which the full length sequence of 397 bp was obtained. The sequenced product revealed only a single nucleotide difference with the Cox2.3 type sequence. Malaysian Society of Parasitology and Tropical Medicine 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45531/1/Molecular%20screening%20for%20Rickettsia%2C%20Anaplasmataceae%20and%20Coxiella%20burnetii%20in%20Rhipicephalus%20sanguineus%20ticks%20from%20Malaysia.pdf Watanabe, Malaika and Nakao, R. and Babjee, Shaik Mohamed Amin and Moktar, Maizatul Akmal and Youn, J. H. and Qiu, Y. and Sugimoto, C. (2015) Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia. Tropical Biomedicine, 32 (2). pp. 390-398. ISSN 0127-5720 https://pubmed.ncbi.nlm.nih.gov/26691268/ |
| spellingShingle | Watanabe, Malaika Nakao, R. Babjee, Shaik Mohamed Amin Moktar, Maizatul Akmal Youn, J. H. Qiu, Y. Sugimoto, C. Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia |
| title | Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia |
| title_full | Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia |
| title_fullStr | Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia |
| title_full_unstemmed | Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia |
| title_short | Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia |
| title_sort | molecular screening for rickettsia anaplasmataceae and coxiella burnetii in rhipicephalus sanguineus ticks from malaysia |
| url | http://psasir.upm.edu.my/id/eprint/45531/1/Molecular%20screening%20for%20Rickettsia%2C%20Anaplasmataceae%20and%20Coxiella%20burnetii%20in%20Rhipicephalus%20sanguineus%20ticks%20from%20Malaysia.pdf |
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