Phenolic rich extract from Clinicanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats
Clinacanthus nutans is used as traditional medicine in Asia but there are limited scientific studies to support its use. In this study, the stem and leaf of C. nutans were extracted using solvents of differing polarities, and their antioxidant capacities were determined using multiple antioxidant as...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/45835/1/NUTAN.pdf |
| _version_ | 1825929602591096832 |
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| author | Ismail, Maznah Mohd Esa, Norhaizan Zawawi, Norhasnida Sarega, Nadarajan Imam, Mustapha Umar Ooi, Der Jiun Chan, Kim Wei |
| author_facet | Ismail, Maznah Mohd Esa, Norhaizan Zawawi, Norhasnida Sarega, Nadarajan Imam, Mustapha Umar Ooi, Der Jiun Chan, Kim Wei |
| author_sort | Ismail, Maznah |
| collection | UPM |
| description | Clinacanthus nutans is used as traditional medicine in Asia but there are limited scientific studies to support its use. In this study, the stem and leaf of C. nutans were extracted using solvents of differing polarities, and their antioxidant capacities were determined using multiple antioxidant assays. The water and aqueous methanolic leaf extracts were further fractionated and their antioxidant capacities and phenolic compositions were tested. Furthermore, the efficacies of the water and aqueous methanolic leaf extracts were tested against hyperlipidemia-induced oxidative stress in rats. Serum and hepatic antioxidant and oxidative stress markers were tested after feeding the rats with high fat diet together with the extracts or simvastatin for 7 weeks. The results indicated that both leaf extracts attenuated oxidative stress through increasing serum antioxidant enzymes activity and upregulating the expression of hepatic antioxidant genes. Multiple phenolic compounds were detected in the extracts and fractions of C. nutans, although protocatechuic acid was one of the most abundant and may have contributed significantly towards the bioactivities of the extracts. However, synergistic effects of different phenolics may have contributed to the overall bioactivities. C. nutans can be a good source of functional ingredients for the management of oxidative stress-related diseases. |
| first_indexed | 2024-03-06T08:59:07Z |
| format | Article |
| id | upm.eprints-45835 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:59:07Z |
| publishDate | 2015 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-458352021-01-25T17:19:38Z http://psasir.upm.edu.my/id/eprint/45835/ Phenolic rich extract from Clinicanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats Ismail, Maznah Mohd Esa, Norhaizan Zawawi, Norhasnida Sarega, Nadarajan Imam, Mustapha Umar Ooi, Der Jiun Chan, Kim Wei Clinacanthus nutans is used as traditional medicine in Asia but there are limited scientific studies to support its use. In this study, the stem and leaf of C. nutans were extracted using solvents of differing polarities, and their antioxidant capacities were determined using multiple antioxidant assays. The water and aqueous methanolic leaf extracts were further fractionated and their antioxidant capacities and phenolic compositions were tested. Furthermore, the efficacies of the water and aqueous methanolic leaf extracts were tested against hyperlipidemia-induced oxidative stress in rats. Serum and hepatic antioxidant and oxidative stress markers were tested after feeding the rats with high fat diet together with the extracts or simvastatin for 7 weeks. The results indicated that both leaf extracts attenuated oxidative stress through increasing serum antioxidant enzymes activity and upregulating the expression of hepatic antioxidant genes. Multiple phenolic compounds were detected in the extracts and fractions of C. nutans, although protocatechuic acid was one of the most abundant and may have contributed significantly towards the bioactivities of the extracts. However, synergistic effects of different phenolics may have contributed to the overall bioactivities. C. nutans can be a good source of functional ingredients for the management of oxidative stress-related diseases. Hindawi Publishing Corporation 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45835/1/NUTAN.pdf Ismail, Maznah and Mohd Esa, Norhaizan and Zawawi, Norhasnida and Sarega, Nadarajan and Imam, Mustapha Umar and Ooi, Der Jiun and Chan, Kim Wei (2015) Phenolic rich extract from Clinicanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats. Oxidative Medicine and Cellular Longevity. pp. 1-16. ISSN 1942-0994; ESSN: 1942-0900 https://www.hindawi.com/journals/omcl/2016/4137908/ 10.1155/2016/4137908 |
| spellingShingle | Ismail, Maznah Mohd Esa, Norhaizan Zawawi, Norhasnida Sarega, Nadarajan Imam, Mustapha Umar Ooi, Der Jiun Chan, Kim Wei Phenolic rich extract from Clinicanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title | Phenolic rich extract from Clinicanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title_full | Phenolic rich extract from Clinicanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title_fullStr | Phenolic rich extract from Clinicanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title_full_unstemmed | Phenolic rich extract from Clinicanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title_short | Phenolic rich extract from Clinicanthus nutans attenuates hyperlipidemia-associated oxidative stress in rats |
| title_sort | phenolic rich extract from clinicanthus nutans attenuates hyperlipidemia associated oxidative stress in rats |
| url | http://psasir.upm.edu.my/id/eprint/45835/1/NUTAN.pdf |
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