Preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides
The aim of this study was to produce and characterise nanosize liposomes containing bioactive peptides with antioxidative and ACE-inhibitory properties, derived from winged bean seeds (WBS) protein. WBS powder was papain-proteolysed, at 70 °C and pH 6.5 for six hours, followed by encapsulation via a...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor and Francis
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/45954/1/Preparation%20and%20characterisation%20of%20nanoliposomes%20containing%20winged%20bean%20seeds%20bioactive%20peptides.pdf |
| _version_ | 1825929618860802048 |
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| author | Chay, Shyan Yea Tan, Wei Kiat Saari, Nazamid |
| author_facet | Chay, Shyan Yea Tan, Wei Kiat Saari, Nazamid |
| author_sort | Chay, Shyan Yea |
| collection | UPM |
| description | The aim of this study was to produce and characterise nanosize liposomes containing bioactive peptides with antioxidative and ACE-inhibitory properties, derived from winged bean seeds (WBS) protein. WBS powder was papain-proteolysed, at 70 °C and pH 6.5 for six hours, followed by encapsulation via a solvent-free heating method. The results showed that the WBS proteolysate was successfully incorporated into spherical, unilamellar liposomal particles, with particle diameter, polydispersity index, zeta potential and encapsulation efficiency of 193.3 ± 0.12 nm, 0.4 ± 0.02 (unit less), -70.5 ± 0.30 mV and 27.6 ± 1.17%, respectively. It also demonstrated good storage stability over eight weeks at 4 °C, indicated by slight increment (15.1%) in particle size and a zeta potential only weaker by 17.2% at the end of the study period. These results suggested the feasibility of entrapping water soluble peptides in hydrophobic liposomal system that, upon optimisation, has the potential to act as bioactive food ingredient. |
| first_indexed | 2024-03-06T08:59:22Z |
| format | Article |
| id | upm.eprints-45954 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:59:22Z |
| publishDate | 2015 |
| publisher | Taylor and Francis |
| record_format | dspace |
| spelling | upm.eprints-459542022-05-19T02:04:21Z http://psasir.upm.edu.my/id/eprint/45954/ Preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides Chay, Shyan Yea Tan, Wei Kiat Saari, Nazamid The aim of this study was to produce and characterise nanosize liposomes containing bioactive peptides with antioxidative and ACE-inhibitory properties, derived from winged bean seeds (WBS) protein. WBS powder was papain-proteolysed, at 70 °C and pH 6.5 for six hours, followed by encapsulation via a solvent-free heating method. The results showed that the WBS proteolysate was successfully incorporated into spherical, unilamellar liposomal particles, with particle diameter, polydispersity index, zeta potential and encapsulation efficiency of 193.3 ± 0.12 nm, 0.4 ± 0.02 (unit less), -70.5 ± 0.30 mV and 27.6 ± 1.17%, respectively. It also demonstrated good storage stability over eight weeks at 4 °C, indicated by slight increment (15.1%) in particle size and a zeta potential only weaker by 17.2% at the end of the study period. These results suggested the feasibility of entrapping water soluble peptides in hydrophobic liposomal system that, upon optimisation, has the potential to act as bioactive food ingredient. Taylor and Francis 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/45954/1/Preparation%20and%20characterisation%20of%20nanoliposomes%20containing%20winged%20bean%20seeds%20bioactive%20peptides.pdf Chay, Shyan Yea and Tan, Wei Kiat and Saari, Nazamid (2015) Preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides. Journal of Microencapsulation, 32 (5). pp. 488-495. ISSN 0265-2048; ESSN: 1464-5246 https://www.tandfonline.com/doi/abs/10.3109/02652048.2015.1057250?journalCode=imnc20 10.3109/02652048.2015.1057250 |
| spellingShingle | Chay, Shyan Yea Tan, Wei Kiat Saari, Nazamid Preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides |
| title | Preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides |
| title_full | Preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides |
| title_fullStr | Preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides |
| title_full_unstemmed | Preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides |
| title_short | Preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides |
| title_sort | preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides |
| url | http://psasir.upm.edu.my/id/eprint/45954/1/Preparation%20and%20characterisation%20of%20nanoliposomes%20containing%20winged%20bean%20seeds%20bioactive%20peptides.pdf |
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