Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus
The purification of a soluble cholinesterase (ChE) from Puntius javanicus liver using affinity chromatography was studied. Affinity matrix was synthesised through the cooling system of ligands procainamide to epoxy-activated Sephacryl 6B and purification process was performed using calibrated flow r...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Friends Science Publishers
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46049/1/Purification%20and%20anticholinesterase%20sensitivity%20of%20cholinesterase%20extracted%20from%20liver%20tissue%20of%20Puntius%20javanicus.pdf |
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| author | Sabullah, Mohd Khalizan Abd. Shukor, Mohd Yunus Shamaan, Nor Aripin Khalid, Ariff Ganzau, Azlan Jualang Sulaiman, Mohd Rosni Jirangon, Hussain Ahmad, Siti Aqlima |
| author_facet | Sabullah, Mohd Khalizan Abd. Shukor, Mohd Yunus Shamaan, Nor Aripin Khalid, Ariff Ganzau, Azlan Jualang Sulaiman, Mohd Rosni Jirangon, Hussain Ahmad, Siti Aqlima |
| author_sort | Sabullah, Mohd Khalizan |
| collection | UPM |
| description | The purification of a soluble cholinesterase (ChE) from Puntius javanicus liver using affinity chromatography was studied. Affinity matrix was synthesised through the cooling system of ligands procainamide to epoxy-activated Sephacryl 6B and purification process was performed using calibrated flow rate at 0.2 mL/min. Non-denaturing electrophoresis condition was employed and the single band native form of ChE was detected at 66.267 kDa after being stained with commasie brilliant blue. ChE detection was performed using gel filtration; ZORBAX column attached to the HPLC with the flow rate of 1 mL/min. Only a single peak was detected at the retention time of 3.720. From the assay evaluation, the final purified ChE procedure displayed the highest sensitivity of detecting the anticholinesterase namely mercury, copper, malaoxon and carbofuran compared to the impure ChE and the results were further discussed in detail to the potential application of ChE from P. javanicus as a biomarker for those toxicants. |
| first_indexed | 2024-03-06T08:59:34Z |
| format | Article |
| id | upm.eprints-46049 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T08:59:34Z |
| publishDate | 2015 |
| publisher | Friends Science Publishers |
| record_format | dspace |
| spelling | upm.eprints-460492018-05-08T01:33:32Z http://psasir.upm.edu.my/id/eprint/46049/ Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus Sabullah, Mohd Khalizan Abd. Shukor, Mohd Yunus Shamaan, Nor Aripin Khalid, Ariff Ganzau, Azlan Jualang Sulaiman, Mohd Rosni Jirangon, Hussain Ahmad, Siti Aqlima The purification of a soluble cholinesterase (ChE) from Puntius javanicus liver using affinity chromatography was studied. Affinity matrix was synthesised through the cooling system of ligands procainamide to epoxy-activated Sephacryl 6B and purification process was performed using calibrated flow rate at 0.2 mL/min. Non-denaturing electrophoresis condition was employed and the single band native form of ChE was detected at 66.267 kDa after being stained with commasie brilliant blue. ChE detection was performed using gel filtration; ZORBAX column attached to the HPLC with the flow rate of 1 mL/min. Only a single peak was detected at the retention time of 3.720. From the assay evaluation, the final purified ChE procedure displayed the highest sensitivity of detecting the anticholinesterase namely mercury, copper, malaoxon and carbofuran compared to the impure ChE and the results were further discussed in detail to the potential application of ChE from P. javanicus as a biomarker for those toxicants. Friends Science Publishers 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46049/1/Purification%20and%20anticholinesterase%20sensitivity%20of%20cholinesterase%20extracted%20from%20liver%20tissue%20of%20Puntius%20javanicus.pdf Sabullah, Mohd Khalizan and Abd. Shukor, Mohd Yunus and Shamaan, Nor Aripin and Khalid, Ariff and Ganzau, Azlan Jualang and Sulaiman, Mohd Rosni and Jirangon, Hussain and Ahmad, Siti Aqlima (2015) Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus. International Journal of Agriculture and Biology, 17 (5). pp. 1025-1030. ISSN 1560-8530; ESSN: 1814-9596 http://www.fspublishers.org 10.17957/IJAB/15.0012 |
| spellingShingle | Sabullah, Mohd Khalizan Abd. Shukor, Mohd Yunus Shamaan, Nor Aripin Khalid, Ariff Ganzau, Azlan Jualang Sulaiman, Mohd Rosni Jirangon, Hussain Ahmad, Siti Aqlima Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus |
| title | Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus |
| title_full | Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus |
| title_fullStr | Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus |
| title_full_unstemmed | Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus |
| title_short | Purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of Puntius javanicus |
| title_sort | purification and anticholinesterase sensitivity of cholinesterase extracted from liver tissue of puntius javanicus |
| url | http://psasir.upm.edu.my/id/eprint/46049/1/Purification%20and%20anticholinesterase%20sensitivity%20of%20cholinesterase%20extracted%20from%20liver%20tissue%20of%20Puntius%20javanicus.pdf |
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