SNF3 as high affinity glucose sensor and its function in supporting the viability of Candida glabrata under glucose-limited environment
Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability. It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites. Therefore, nutrient sensing particularly glucose sensing is thought to be...
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| Format: | Article |
| Language: | English |
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Frontiers Media
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46310/1/SNF3%20as%20high%20affinity%20glucose%20sensor%20and%20its%20function%20in%20supporting%20the%20viability%20of%20Candida%20glabrata%20under%20glucose-limited%20environment.pdf |
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| author | Ng, Tzu Shan Chew, Shu Yih Rangasamy, Premmala Mohd Desa, Mohd N. Sandai, Doblin Chong, Pei Pei Than, Leslie Thian Lung |
| author_facet | Ng, Tzu Shan Chew, Shu Yih Rangasamy, Premmala Mohd Desa, Mohd N. Sandai, Doblin Chong, Pei Pei Than, Leslie Thian Lung |
| author_sort | Ng, Tzu Shan |
| collection | UPM |
| description | Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability. It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites. Therefore, nutrient sensing particularly glucose sensing is thought to be crucial in contributing to the development and fitness of the pathogen. This study aimed to elucidate the role of SNF3 (Sucrose Non Fermenting 3) as a glucose sensor and its possible role in contributing to the fitness and survivability of C. glabrata in glucose-limited environment. The SNF3 knockout strain was constructed and subjected to different glucose concentrations to evaluate its growth, biofilm formation, amphotericin B susceptibility, ex vivo survivability and effects on the transcriptional profiling of the sugar receptor repressor (SRR) pathway-related genes. The CgSNF3Δ strain showed a retarded growth in low glucose environments (0.01 and 0.1%) in both fermentation and respiration-preferred conditions but grew well in high glucose concentration environments (1 and 2%). It was also found to be more susceptible to amphotericin B in low glucose environment (0.1%) and macrophage engulfment but showed no difference in the biofilm formation capability. The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine). Overall, the deletion of SNF3 causes significant reduction in the ability of C. glabrata to sense limited surrounding glucose and consequently disrupts its competency to transport and perform the uptake of this critical nutrient. This study highlighted the role of SNF3 as a high affinity glucose sensor and its role in aiding the survivability of C. glabrata particularly in glucose limited environment. |
| first_indexed | 2024-03-06T09:00:07Z |
| format | Article |
| id | upm.eprints-46310 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:00:07Z |
| publishDate | 2015 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | upm.eprints-463102022-06-17T04:12:25Z http://psasir.upm.edu.my/id/eprint/46310/ SNF3 as high affinity glucose sensor and its function in supporting the viability of Candida glabrata under glucose-limited environment Ng, Tzu Shan Chew, Shu Yih Rangasamy, Premmala Mohd Desa, Mohd N. Sandai, Doblin Chong, Pei Pei Than, Leslie Thian Lung Candida glabrata is an emerging human fungal pathogen that has efficacious nutrient sensing and responsiveness ability. It can be seen through its ability to thrive in diverse range of nutrient limited-human anatomical sites. Therefore, nutrient sensing particularly glucose sensing is thought to be crucial in contributing to the development and fitness of the pathogen. This study aimed to elucidate the role of SNF3 (Sucrose Non Fermenting 3) as a glucose sensor and its possible role in contributing to the fitness and survivability of C. glabrata in glucose-limited environment. The SNF3 knockout strain was constructed and subjected to different glucose concentrations to evaluate its growth, biofilm formation, amphotericin B susceptibility, ex vivo survivability and effects on the transcriptional profiling of the sugar receptor repressor (SRR) pathway-related genes. The CgSNF3Δ strain showed a retarded growth in low glucose environments (0.01 and 0.1%) in both fermentation and respiration-preferred conditions but grew well in high glucose concentration environments (1 and 2%). It was also found to be more susceptible to amphotericin B in low glucose environment (0.1%) and macrophage engulfment but showed no difference in the biofilm formation capability. The deletion of SNF3 also resulted in the down-regulation of about half of hexose transporters genes (four out of nine). Overall, the deletion of SNF3 causes significant reduction in the ability of C. glabrata to sense limited surrounding glucose and consequently disrupts its competency to transport and perform the uptake of this critical nutrient. This study highlighted the role of SNF3 as a high affinity glucose sensor and its role in aiding the survivability of C. glabrata particularly in glucose limited environment. Frontiers Media 2015-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46310/1/SNF3%20as%20high%20affinity%20glucose%20sensor%20and%20its%20function%20in%20supporting%20the%20viability%20of%20Candida%20glabrata%20under%20glucose-limited%20environment.pdf Ng, Tzu Shan and Chew, Shu Yih and Rangasamy, Premmala and Mohd Desa, Mohd N. and Sandai, Doblin and Chong, Pei Pei and Than, Leslie Thian Lung (2015) SNF3 as high affinity glucose sensor and its function in supporting the viability of Candida glabrata under glucose-limited environment. Frontiers in Microbiology, 6. art. no. 1334. pp. 1-12. ISSN 1664-302X https://www.frontiersin.org/articles/10.3389/fmicb.2015.01334/full 10.3389/fmicb.2015.01334 |
| spellingShingle | Ng, Tzu Shan Chew, Shu Yih Rangasamy, Premmala Mohd Desa, Mohd N. Sandai, Doblin Chong, Pei Pei Than, Leslie Thian Lung SNF3 as high affinity glucose sensor and its function in supporting the viability of Candida glabrata under glucose-limited environment |
| title | SNF3 as high affinity glucose sensor and its function in supporting the viability of Candida glabrata under glucose-limited environment |
| title_full | SNF3 as high affinity glucose sensor and its function in supporting the viability of Candida glabrata under glucose-limited environment |
| title_fullStr | SNF3 as high affinity glucose sensor and its function in supporting the viability of Candida glabrata under glucose-limited environment |
| title_full_unstemmed | SNF3 as high affinity glucose sensor and its function in supporting the viability of Candida glabrata under glucose-limited environment |
| title_short | SNF3 as high affinity glucose sensor and its function in supporting the viability of Candida glabrata under glucose-limited environment |
| title_sort | snf3 as high affinity glucose sensor and its function in supporting the viability of candida glabrata under glucose limited environment |
| url | http://psasir.upm.edu.my/id/eprint/46310/1/SNF3%20as%20high%20affinity%20glucose%20sensor%20and%20its%20function%20in%20supporting%20the%20viability%20of%20Candida%20glabrata%20under%20glucose-limited%20environment.pdf |
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