Study of genetic variation of some eggplant cultivars through RAPD-PCR molecular markers and its relatedness to phomopsis blight disease reaction
Disease susceptibility and genetic variability in 10 eggplant genotypes were studied after inoculating Phomopsis vexans under confined field conditions. Random amplified polymorphic DNA (RAPD) markers were used to assess genetic variation and relationships among eggplant genotypes. The disease index...
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| Format: | Article |
| Language: | English |
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Fundacao de Pesquisas Cientificas de Ribeirao Preto
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46413/1/Study%20of%20genetic%20variation%20of%20some%20eggplant%20cultivars%20through%20RAPD-PCR%20molecular%20markers%20and%20its%20relatedness%20to%20phomopsis%20blight%20disease%20reaction.pdf |
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| author | Asad, H. A. Meah, M. B. Begum, S. N. Khalil, M. I. Yusop, Mohd Rafii Latif, M. A. |
| author_facet | Asad, H. A. Meah, M. B. Begum, S. N. Khalil, M. I. Yusop, Mohd Rafii Latif, M. A. |
| author_sort | Asad, H. A. |
| collection | UPM |
| description | Disease susceptibility and genetic variability in 10 eggplant genotypes were studied after inoculating Phomopsis vexans under confined field conditions. Random amplified polymorphic DNA (RAPD) markers were used to assess genetic variation and relationships among eggplant genotypes. The disease index of leaves ranged 0.208-13.79%, while fruit infection ranged 2.15-42.76%. Two varieties, Dohazari G and Laffa S, were found to be susceptible, 6 were moderately resistant, 1 was moderately susceptible, and BAU Begun-1 was resistant to P. vexans. Amplification of genomic DNA by using 3 RAPD primers produced 20 bands: 14 (70%) were polymorphic and 6 (30%) were monomorphic. The highest intra-variety similarity indices values were found in ISD 006, Ishurdi L, Jessore L, and BAU Begun-1 (100%), while the lowest was in Dohazari G (90%). The lowest genetic distance (0.0513) and the highest genetic identity (0.9500) were observed between the ISD 006 and Ishurdi L combinations. A comparatively higher genetic distance (0.3724) and the lowest genetic identity (0.6891) were observed between the ISD 006 and Dohazari G combinations. A dendogram was constructed based on Nei’s genetic distance, which produced 2 main clusters of the genotypes - Cluster I: ISD 006, Ishurdi L, Marich begun L, BAU Begun-1, Marich begun S, and Chega and Cluster 2: Laffa S, Dohazari G, Jessore L, and Singhnath. Genetic variation and its relationship with disease susceptibility were assessed using RAPD markers, to develop disease-resistant varieties and improve eggplant crops. |
| first_indexed | 2024-03-06T09:00:20Z |
| format | Article |
| id | upm.eprints-46413 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:00:20Z |
| publishDate | 2015 |
| publisher | Fundacao de Pesquisas Cientificas de Ribeirao Preto |
| record_format | dspace |
| spelling | upm.eprints-464132018-02-27T08:21:09Z http://psasir.upm.edu.my/id/eprint/46413/ Study of genetic variation of some eggplant cultivars through RAPD-PCR molecular markers and its relatedness to phomopsis blight disease reaction Asad, H. A. Meah, M. B. Begum, S. N. Khalil, M. I. Yusop, Mohd Rafii Latif, M. A. Disease susceptibility and genetic variability in 10 eggplant genotypes were studied after inoculating Phomopsis vexans under confined field conditions. Random amplified polymorphic DNA (RAPD) markers were used to assess genetic variation and relationships among eggplant genotypes. The disease index of leaves ranged 0.208-13.79%, while fruit infection ranged 2.15-42.76%. Two varieties, Dohazari G and Laffa S, were found to be susceptible, 6 were moderately resistant, 1 was moderately susceptible, and BAU Begun-1 was resistant to P. vexans. Amplification of genomic DNA by using 3 RAPD primers produced 20 bands: 14 (70%) were polymorphic and 6 (30%) were monomorphic. The highest intra-variety similarity indices values were found in ISD 006, Ishurdi L, Jessore L, and BAU Begun-1 (100%), while the lowest was in Dohazari G (90%). The lowest genetic distance (0.0513) and the highest genetic identity (0.9500) were observed between the ISD 006 and Ishurdi L combinations. A comparatively higher genetic distance (0.3724) and the lowest genetic identity (0.6891) were observed between the ISD 006 and Dohazari G combinations. A dendogram was constructed based on Nei’s genetic distance, which produced 2 main clusters of the genotypes - Cluster I: ISD 006, Ishurdi L, Marich begun L, BAU Begun-1, Marich begun S, and Chega and Cluster 2: Laffa S, Dohazari G, Jessore L, and Singhnath. Genetic variation and its relationship with disease susceptibility were assessed using RAPD markers, to develop disease-resistant varieties and improve eggplant crops. Fundacao de Pesquisas Cientificas de Ribeirao Preto 2015-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46413/1/Study%20of%20genetic%20variation%20of%20some%20eggplant%20cultivars%20through%20RAPD-PCR%20molecular%20markers%20and%20its%20relatedness%20to%20phomopsis%20blight%20disease%20reaction.pdf Asad, H. A. and Meah, M. B. and Begum, S. N. and Khalil, M. I. and Yusop, Mohd Rafii and Latif, M. A. (2015) Study of genetic variation of some eggplant cultivars through RAPD-PCR molecular markers and its relatedness to phomopsis blight disease reaction. Genetics and Molecular Research, 14 (4). pp. 17007-17018. ISSN 1676-5680 https://www.geneticsmr.com/ 10.4238/2015.December.16.1 |
| spellingShingle | Asad, H. A. Meah, M. B. Begum, S. N. Khalil, M. I. Yusop, Mohd Rafii Latif, M. A. Study of genetic variation of some eggplant cultivars through RAPD-PCR molecular markers and its relatedness to phomopsis blight disease reaction |
| title | Study of genetic variation of some eggplant cultivars through RAPD-PCR molecular markers and its relatedness to phomopsis blight disease reaction |
| title_full | Study of genetic variation of some eggplant cultivars through RAPD-PCR molecular markers and its relatedness to phomopsis blight disease reaction |
| title_fullStr | Study of genetic variation of some eggplant cultivars through RAPD-PCR molecular markers and its relatedness to phomopsis blight disease reaction |
| title_full_unstemmed | Study of genetic variation of some eggplant cultivars through RAPD-PCR molecular markers and its relatedness to phomopsis blight disease reaction |
| title_short | Study of genetic variation of some eggplant cultivars through RAPD-PCR molecular markers and its relatedness to phomopsis blight disease reaction |
| title_sort | study of genetic variation of some eggplant cultivars through rapd pcr molecular markers and its relatedness to phomopsis blight disease reaction |
| url | http://psasir.upm.edu.my/id/eprint/46413/1/Study%20of%20genetic%20variation%20of%20some%20eggplant%20cultivars%20through%20RAPD-PCR%20molecular%20markers%20and%20its%20relatedness%20to%20phomopsis%20blight%20disease%20reaction.pdf |
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