Synergy effect of nanocrystalline cellulose for the biosensing detection of glucose
Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated. Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor per...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46449/1/Synergy%20effect%20of%20nanocrystalline%20cellulose%20for%20the%20biosensing%20detection%20of%20glucose.pdf |
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| author | Esmaeili, Chakavak Abdi, Mahnaz M. Mathew, Aji P. Jonoobi, Mehdi Oksman, Kristiina Rezayi, Majid |
| author_facet | Esmaeili, Chakavak Abdi, Mahnaz M. Mathew, Aji P. Jonoobi, Mehdi Oksman, Kristiina Rezayi, Majid |
| author_sort | Esmaeili, Chakavak |
| collection | UPM |
| description | Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated. Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor performance. Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics. The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM−1), with a high dynamic response ranging from 1.0 to 20 mM glucose. The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples. This sensor displays an acceptable reproducibility and stability over time. The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%. |
| first_indexed | 2024-03-06T09:00:24Z |
| format | Article |
| id | upm.eprints-46449 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:00:24Z |
| publishDate | 2015 |
| publisher | MDPI AG |
| record_format | dspace |
| spelling | upm.eprints-464492018-02-27T06:15:44Z http://psasir.upm.edu.my/id/eprint/46449/ Synergy effect of nanocrystalline cellulose for the biosensing detection of glucose Esmaeili, Chakavak Abdi, Mahnaz M. Mathew, Aji P. Jonoobi, Mehdi Oksman, Kristiina Rezayi, Majid Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated. Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor performance. Field emission scanning electron microscopy (FESEM) images showed that fibers were nanosized and porous, which is appropriate for accommodating enzymes and increasing electron transfer kinetics. The voltammetric results showed that the native structure and biocatalytic activity of GOx immobilized on the PPy-CNC nanocomposite remained and exhibited a high sensitivity (ca. 0.73 μA·mM−1), with a high dynamic response ranging from 1.0 to 20 mM glucose. The modified glucose biosensor exhibits a limit of detection (LOD) of (50 ± 10) µM and also excludes interfering species, such as ascorbic acid, uric acid, and cholesterol, which makes this sensor suitable for glucose determination in real samples. This sensor displays an acceptable reproducibility and stability over time. The current response was maintained over 95% of the initial value after 17 days, and the current difference measurement obtained using different electrodes provided a relative standard deviation (RSD) of 4.47%. MDPI AG 2015 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46449/1/Synergy%20effect%20of%20nanocrystalline%20cellulose%20for%20the%20biosensing%20detection%20of%20glucose.pdf Esmaeili, Chakavak and Abdi, Mahnaz M. and Mathew, Aji P. and Jonoobi, Mehdi and Oksman, Kristiina and Rezayi, Majid (2015) Synergy effect of nanocrystalline cellulose for the biosensing detection of glucose. Sensors, 15 (10). pp. 24681-24697. ISSN 1424-8220 http://www.mdpi.com/journal/sensors 10.3390/s151024681 |
| spellingShingle | Esmaeili, Chakavak Abdi, Mahnaz M. Mathew, Aji P. Jonoobi, Mehdi Oksman, Kristiina Rezayi, Majid Synergy effect of nanocrystalline cellulose for the biosensing detection of glucose |
| title | Synergy effect of nanocrystalline cellulose for the biosensing detection of glucose |
| title_full | Synergy effect of nanocrystalline cellulose for the biosensing detection of glucose |
| title_fullStr | Synergy effect of nanocrystalline cellulose for the biosensing detection of glucose |
| title_full_unstemmed | Synergy effect of nanocrystalline cellulose for the biosensing detection of glucose |
| title_short | Synergy effect of nanocrystalline cellulose for the biosensing detection of glucose |
| title_sort | synergy effect of nanocrystalline cellulose for the biosensing detection of glucose |
| url | http://psasir.upm.edu.my/id/eprint/46449/1/Synergy%20effect%20of%20nanocrystalline%20cellulose%20for%20the%20biosensing%20detection%20of%20glucose.pdf |
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