The prophylactic effect of probiotic enterococcus lactis IW5 against different human cancel cells
Enterococcus lactis IW5 was obtained from human gut and the potential probiotic characteristics of this organism were then evaluated. Results showed that this strain was highly resistant to low pH and high bile salt and adhered strongly to Caco-2 human epithelial colorectal cell lines. The supernata...
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| Format: | Article |
| Language: | English |
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Frontiers
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/46670/1/The%20prophylactic%20effect%20of%20probiotic%20enterococcus%20lactis%20IW5%20against%20different%20human%20cancel%20cells.pdf |
| _version_ | 1796974710004645888 |
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| author | Nami, Yousef Haghshenas, Babak Haghshenas, Minoo Abdullah, Norhafizah Khosroushahi, Ahmad Yari |
| author_facet | Nami, Yousef Haghshenas, Babak Haghshenas, Minoo Abdullah, Norhafizah Khosroushahi, Ahmad Yari |
| author_sort | Nami, Yousef |
| collection | UPM |
| description | Enterococcus lactis IW5 was obtained from human gut and the potential probiotic characteristics of this organism were then evaluated. Results showed that this strain was highly resistant to low pH and high bile salt and adhered strongly to Caco-2 human epithelial colorectal cell lines. The supernatant of E. lactis IW5 strongly inhibited the growth of several pathogenic bacteria and decreased the viability of different cancer cells, such as HeLa, MCF-7, AGS, HT-29, and Caco-2. Conversely, E. lactis IW5 did not inhibit the viability of normal FHs-74 cells. This strain did not generate toxic enzymes, including β-glucosidase, β-glucuronidase, and N-acetyl-β-glucosaminidase and was highly susceptible to ampicillin, gentamycin, penicillin, vancomycin, clindamycin, sulfamethoxazol, and chloramphenicol but resistant to erythromycin and tetracyclin. This study provided evidence for the effect of E. lactis IW5 on cancer cells. Therefore, E. lactis IW5, as a bioactive therapeutics, should be subjected to other relevant tests to verify the therapeutic suitability of this strain for clinical applications. |
| first_indexed | 2024-03-06T09:00:56Z |
| format | Article |
| id | upm.eprints-46670 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:00:56Z |
| publishDate | 2015 |
| publisher | Frontiers |
| record_format | dspace |
| spelling | upm.eprints-466702018-03-30T04:54:38Z http://psasir.upm.edu.my/id/eprint/46670/ The prophylactic effect of probiotic enterococcus lactis IW5 against different human cancel cells Nami, Yousef Haghshenas, Babak Haghshenas, Minoo Abdullah, Norhafizah Khosroushahi, Ahmad Yari Enterococcus lactis IW5 was obtained from human gut and the potential probiotic characteristics of this organism were then evaluated. Results showed that this strain was highly resistant to low pH and high bile salt and adhered strongly to Caco-2 human epithelial colorectal cell lines. The supernatant of E. lactis IW5 strongly inhibited the growth of several pathogenic bacteria and decreased the viability of different cancer cells, such as HeLa, MCF-7, AGS, HT-29, and Caco-2. Conversely, E. lactis IW5 did not inhibit the viability of normal FHs-74 cells. This strain did not generate toxic enzymes, including β-glucosidase, β-glucuronidase, and N-acetyl-β-glucosaminidase and was highly susceptible to ampicillin, gentamycin, penicillin, vancomycin, clindamycin, sulfamethoxazol, and chloramphenicol but resistant to erythromycin and tetracyclin. This study provided evidence for the effect of E. lactis IW5 on cancer cells. Therefore, E. lactis IW5, as a bioactive therapeutics, should be subjected to other relevant tests to verify the therapeutic suitability of this strain for clinical applications. Frontiers 2015-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46670/1/The%20prophylactic%20effect%20of%20probiotic%20enterococcus%20lactis%20IW5%20against%20different%20human%20cancel%20cells.pdf Nami, Yousef and Haghshenas, Babak and Haghshenas, Minoo and Abdullah, Norhafizah and Khosroushahi, Ahmad Yari (2015) The prophylactic effect of probiotic enterococcus lactis IW5 against different human cancel cells. Frontiers in Microbiology, 6. pp. 1-11. ISSN 1664-302X; ESSN: 1664-302X 10.3389/fmicb.2015.01317 |
| spellingShingle | Nami, Yousef Haghshenas, Babak Haghshenas, Minoo Abdullah, Norhafizah Khosroushahi, Ahmad Yari The prophylactic effect of probiotic enterococcus lactis IW5 against different human cancel cells |
| title | The prophylactic effect of probiotic enterococcus lactis IW5 against different human cancel cells |
| title_full | The prophylactic effect of probiotic enterococcus lactis IW5 against different human cancel cells |
| title_fullStr | The prophylactic effect of probiotic enterococcus lactis IW5 against different human cancel cells |
| title_full_unstemmed | The prophylactic effect of probiotic enterococcus lactis IW5 against different human cancel cells |
| title_short | The prophylactic effect of probiotic enterococcus lactis IW5 against different human cancel cells |
| title_sort | prophylactic effect of probiotic enterococcus lactis iw5 against different human cancel cells |
| url | http://psasir.upm.edu.my/id/eprint/46670/1/The%20prophylactic%20effect%20of%20probiotic%20enterococcus%20lactis%20IW5%20against%20different%20human%20cancel%20cells.pdf |
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