An alternative bioassay using Anabas testudineus (climbing perch) colinesterase for metal ions detection
Climbing Perch or its scientific name, Anabas testudineus is one of the freshwater fish belonging to the family of Anabantidae. It is widely distributed in ponds, swamps and estuaries in Asia. In this study, cholinesterase (ChE) was partially purified from the liver of A. testudineus through ion exc...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/47817/1/%2814%29.pdf |
| _version_ | 1825929879206494208 |
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| author | Ahmad, Siti Aqlima Wong, Y. F. Abd Shukor, Mohd Yunus Sabullah, Mohd Khalizan Yasid, N. A. Hayat, N. M. Shamaan, Nor Aripin Khalid, Ariff Syed, Mohd Arif |
| author_facet | Ahmad, Siti Aqlima Wong, Y. F. Abd Shukor, Mohd Yunus Sabullah, Mohd Khalizan Yasid, N. A. Hayat, N. M. Shamaan, Nor Aripin Khalid, Ariff Syed, Mohd Arif |
| author_sort | Ahmad, Siti Aqlima |
| collection | UPM |
| description | Climbing Perch or its scientific name, Anabas testudineus is one of the freshwater fish belonging to the family of Anabantidae. It is widely distributed in ponds, swamps and estuaries in Asia. In this study, cholinesterase (ChE) was partially purified from the liver of A. testudineus through ion exchange chromatography. This purification method provided a recovery yield of 5.36% with a purification fold of 6.6. The optimum conditions for ChE assay were identified to be 2.5 mM of butyrylthiocholine iodide (BTC) with pH 8.0 in Tris-HCl buffer at 40°C. Substrate specificity profile also indicated that ChE favours BTC as substrate because it records the highest catalytic efficiency (Vmax/Km). Protein analysis through Native-PAGE showed that ion exchange chromatography is an effective method to partially purify ChE. Metal ion inhibition tests were conducted and mercury (Hg) was found to show the highest inhibition effect (87.30%) whereas lead (Pb) shows the lowest inhibition effect (28.01%). All these findings showed that partially purified ChE from the liver of A. testudineus is suitable to be used as a bioindicator to detect the presence of metal ions. |
| first_indexed | 2024-03-06T09:03:12Z |
| format | Article |
| id | upm.eprints-47817 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:03:12Z |
| publishDate | 2016 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-478172016-08-03T03:08:02Z http://psasir.upm.edu.my/id/eprint/47817/ An alternative bioassay using Anabas testudineus (climbing perch) colinesterase for metal ions detection Ahmad, Siti Aqlima Wong, Y. F. Abd Shukor, Mohd Yunus Sabullah, Mohd Khalizan Yasid, N. A. Hayat, N. M. Shamaan, Nor Aripin Khalid, Ariff Syed, Mohd Arif Climbing Perch or its scientific name, Anabas testudineus is one of the freshwater fish belonging to the family of Anabantidae. It is widely distributed in ponds, swamps and estuaries in Asia. In this study, cholinesterase (ChE) was partially purified from the liver of A. testudineus through ion exchange chromatography. This purification method provided a recovery yield of 5.36% with a purification fold of 6.6. The optimum conditions for ChE assay were identified to be 2.5 mM of butyrylthiocholine iodide (BTC) with pH 8.0 in Tris-HCl buffer at 40°C. Substrate specificity profile also indicated that ChE favours BTC as substrate because it records the highest catalytic efficiency (Vmax/Km). Protein analysis through Native-PAGE showed that ion exchange chromatography is an effective method to partially purify ChE. Metal ion inhibition tests were conducted and mercury (Hg) was found to show the highest inhibition effect (87.30%) whereas lead (Pb) shows the lowest inhibition effect (28.01%). All these findings showed that partially purified ChE from the liver of A. testudineus is suitable to be used as a bioindicator to detect the presence of metal ions. Faculty of Food Science and Technology, Universiti Putra Malaysia 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/47817/1/%2814%29.pdf Ahmad, Siti Aqlima and Wong, Y. F. and Abd Shukor, Mohd Yunus and Sabullah, Mohd Khalizan and Yasid, N. A. and Hayat, N. M. and Shamaan, Nor Aripin and Khalid, Ariff and Syed, Mohd Arif (2016) An alternative bioassay using Anabas testudineus (climbing perch) colinesterase for metal ions detection. International Food Research Journal, 23 (4). pp. 1446-1452. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/23%20(04)%202016/(14).pdf |
| spellingShingle | Ahmad, Siti Aqlima Wong, Y. F. Abd Shukor, Mohd Yunus Sabullah, Mohd Khalizan Yasid, N. A. Hayat, N. M. Shamaan, Nor Aripin Khalid, Ariff Syed, Mohd Arif An alternative bioassay using Anabas testudineus (climbing perch) colinesterase for metal ions detection |
| title | An alternative bioassay using Anabas testudineus (climbing perch) colinesterase for metal ions detection |
| title_full | An alternative bioassay using Anabas testudineus (climbing perch) colinesterase for metal ions detection |
| title_fullStr | An alternative bioassay using Anabas testudineus (climbing perch) colinesterase for metal ions detection |
| title_full_unstemmed | An alternative bioassay using Anabas testudineus (climbing perch) colinesterase for metal ions detection |
| title_short | An alternative bioassay using Anabas testudineus (climbing perch) colinesterase for metal ions detection |
| title_sort | alternative bioassay using anabas testudineus climbing perch colinesterase for metal ions detection |
| url | http://psasir.upm.edu.my/id/eprint/47817/1/%2814%29.pdf |
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