Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE)
Nine genomic DNA of Salmonella agona isolated from poultry sources were characterised by three variations of PCR-based techniques [polymerase chain reaction (PCR)-ribotyping, enterobacterial repetitive intergenic consensuspolymerase chain reaction (ERIC-PCR), and random amplified polymorphic DNA-pol...
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| Format: | Article |
| Language: | English |
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Malaysian Agricultural Research and Development Institute (MARDI)
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/48891/1/Molecular%20typing%20among%20poultry%20isolates%20of%20Salmonella%20agona%20by%20PCR-based%20techniques%20and%20pulsed%20field%20gel%20electrophoresis%20%28PFGE%29.pdf |
| _version_ | 1825930094075445248 |
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| author | Abd Mutalib, Sahilah Radu, Son Lum, Keng Yeang Lihan, Samuel |
| author_facet | Abd Mutalib, Sahilah Radu, Son Lum, Keng Yeang Lihan, Samuel |
| author_sort | Abd Mutalib, Sahilah |
| collection | UPM |
| description | Nine genomic DNA of Salmonella agona isolated from poultry sources were characterised by three variations of PCR-based techniques [polymerase chain reaction (PCR)-ribotyping, enterobacterial repetitive intergenic consensuspolymerase chain reaction (ERIC-PCR), and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR)] and pulsed field gel electrophoresis (PFGE). These isolates originated from three different states of Peninsular Malaysia (Pulau Pinang, Negeri Sembilan and Selangor). The results of PCR-ribotyping, ERIC-PCR, RADP-PCR and PFGE to differentiate between nine isolates of S. agona were compared. The isolates were separated into three different genome types by PCR-ribotyping, nine and eight genome types by ERIC-PCR and PFGE, respectively. |
| first_indexed | 2024-03-06T09:06:23Z |
| format | Article |
| id | upm.eprints-48891 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:06:23Z |
| publishDate | 2003 |
| publisher | Malaysian Agricultural Research and Development Institute (MARDI) |
| record_format | dspace |
| spelling | upm.eprints-488912016-12-29T09:48:20Z http://psasir.upm.edu.my/id/eprint/48891/ Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE) Abd Mutalib, Sahilah Radu, Son Lum, Keng Yeang Lihan, Samuel Nine genomic DNA of Salmonella agona isolated from poultry sources were characterised by three variations of PCR-based techniques [polymerase chain reaction (PCR)-ribotyping, enterobacterial repetitive intergenic consensuspolymerase chain reaction (ERIC-PCR), and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR)] and pulsed field gel electrophoresis (PFGE). These isolates originated from three different states of Peninsular Malaysia (Pulau Pinang, Negeri Sembilan and Selangor). The results of PCR-ribotyping, ERIC-PCR, RADP-PCR and PFGE to differentiate between nine isolates of S. agona were compared. The isolates were separated into three different genome types by PCR-ribotyping, nine and eight genome types by ERIC-PCR and PFGE, respectively. Malaysian Agricultural Research and Development Institute (MARDI) 2003 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/48891/1/Molecular%20typing%20among%20poultry%20isolates%20of%20Salmonella%20agona%20by%20PCR-based%20techniques%20and%20pulsed%20field%20gel%20electrophoresis%20%28PFGE%29.pdf Abd Mutalib, Sahilah and Radu, Son and Lum, Keng Yeang and Lihan, Samuel (2003) Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE). Journal of Tropical Agriculture and Food Science, 31 (1). pp. 35-42. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/77-2003/volume-31-no1/458-310109 |
| spellingShingle | Abd Mutalib, Sahilah Radu, Son Lum, Keng Yeang Lihan, Samuel Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE) |
| title | Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE) |
| title_full | Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE) |
| title_fullStr | Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE) |
| title_full_unstemmed | Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE) |
| title_short | Molecular typing among poultry isolates of Salmonella agona by PCR-based techniques and pulsed field gel electrophoresis (PFGE) |
| title_sort | molecular typing among poultry isolates of salmonella agona by pcr based techniques and pulsed field gel electrophoresis pfge |
| url | http://psasir.upm.edu.my/id/eprint/48891/1/Molecular%20typing%20among%20poultry%20isolates%20of%20Salmonella%20agona%20by%20PCR-based%20techniques%20and%20pulsed%20field%20gel%20electrophoresis%20%28PFGE%29.pdf |
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