Typing of Ralstonia solanacearum isolated from tomato by antibiotic susceptibility, plasmid profiling and PCR-based techniques of RAPD and ERIC
The epidemiological characteristics of Ralstonia solanacearum isolated from tomato plants in Sarawak and Selangor were studied. The epidemiological analysis of the strains was carried out through antibiotic resistant pattern, plasmid profiles, randomly amplified polymorphic DNA (RAPD) and enterobact...
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| Format: | Article |
| Language: | English |
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Malaysian Agricultural Research and Development Institute (MARDI)
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/48936/1/Typing%20of%20Ralstonia%20solanacearum%20isolated%20from%20tomato%20by%20antibiotic%20susceptibility%2C%20plasmid%20profiling%20and%20PCR-based%20techniques%20of%20RAPD%20and%20ERIC.pdf |
| _version_ | 1825930103065935872 |
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| author | Abd Mutalib, Sahilah Sadi, Tosiah Radu, Son Ooi, Wai Ling Queen, C. Y. K. Lim, Jeffrey Seng Heng Chua, S. C. Mohd Tamin, Mohd Senawi |
| author_facet | Abd Mutalib, Sahilah Sadi, Tosiah Radu, Son Ooi, Wai Ling Queen, C. Y. K. Lim, Jeffrey Seng Heng Chua, S. C. Mohd Tamin, Mohd Senawi |
| author_sort | Abd Mutalib, Sahilah |
| collection | UPM |
| description | The epidemiological characteristics of Ralstonia solanacearum isolated from tomato plants in Sarawak and Selangor were studied. The epidemiological analysis of the strains was carried out through antibiotic resistant pattern, plasmid profiles, randomly amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC) methods. Six strains were susceptible to all antibiotics tested, whereas the 10 strains that were resistant to one or more antibiotics were grouped into six antibiotic resistance patterns. Small single plasmid of 7.2 Mda and 9.2 Mda were detected among the nine plasmid containing strains, enabling them to be grouped into only two plasmid patterns. In the polymerase chain reaction, based methods using RAPD and ERIC, two strains were untypable by RAPD, whereas all were typable by ERIC. In this study, a wide diversity of R. solanacearum strains was examined. ERIC analysis demonstrated the clonal relationship between isolates from tomato plants in Sarawak and Selangor. The existence of similar R. solanacearum strains in tomato plants from two very distant locations should be considered if tomato strain fingerprint results were to be used to help trace the vehicles for transmission. |
| first_indexed | 2024-03-06T09:06:31Z |
| format | Article |
| id | upm.eprints-48936 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:06:31Z |
| publishDate | 2005 |
| publisher | Malaysian Agricultural Research and Development Institute (MARDI) |
| record_format | dspace |
| spelling | upm.eprints-489362016-12-29T09:58:48Z http://psasir.upm.edu.my/id/eprint/48936/ Typing of Ralstonia solanacearum isolated from tomato by antibiotic susceptibility, plasmid profiling and PCR-based techniques of RAPD and ERIC Abd Mutalib, Sahilah Sadi, Tosiah Radu, Son Ooi, Wai Ling Queen, C. Y. K. Lim, Jeffrey Seng Heng Chua, S. C. Mohd Tamin, Mohd Senawi The epidemiological characteristics of Ralstonia solanacearum isolated from tomato plants in Sarawak and Selangor were studied. The epidemiological analysis of the strains was carried out through antibiotic resistant pattern, plasmid profiles, randomly amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC) methods. Six strains were susceptible to all antibiotics tested, whereas the 10 strains that were resistant to one or more antibiotics were grouped into six antibiotic resistance patterns. Small single plasmid of 7.2 Mda and 9.2 Mda were detected among the nine plasmid containing strains, enabling them to be grouped into only two plasmid patterns. In the polymerase chain reaction, based methods using RAPD and ERIC, two strains were untypable by RAPD, whereas all were typable by ERIC. In this study, a wide diversity of R. solanacearum strains was examined. ERIC analysis demonstrated the clonal relationship between isolates from tomato plants in Sarawak and Selangor. The existence of similar R. solanacearum strains in tomato plants from two very distant locations should be considered if tomato strain fingerprint results were to be used to help trace the vehicles for transmission. Malaysian Agricultural Research and Development Institute (MARDI) 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/48936/1/Typing%20of%20Ralstonia%20solanacearum%20isolated%20from%20tomato%20by%20antibiotic%20susceptibility%2C%20plasmid%20profiling%20and%20PCR-based%20techniques%20of%20RAPD%20and%20ERIC.pdf Abd Mutalib, Sahilah and Sadi, Tosiah and Radu, Son and Ooi, Wai Ling and Queen, C. Y. K. and Lim, Jeffrey Seng Heng and Chua, S. C. and Mohd Tamin, Mohd Senawi (2005) Typing of Ralstonia solanacearum isolated from tomato by antibiotic susceptibility, plasmid profiling and PCR-based techniques of RAPD and ERIC. Journal of Tropical Agriculture and Food Science, 33 (1). pp. 73-82. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/68-2005/volume-33-no1/833-330117 |
| spellingShingle | Abd Mutalib, Sahilah Sadi, Tosiah Radu, Son Ooi, Wai Ling Queen, C. Y. K. Lim, Jeffrey Seng Heng Chua, S. C. Mohd Tamin, Mohd Senawi Typing of Ralstonia solanacearum isolated from tomato by antibiotic susceptibility, plasmid profiling and PCR-based techniques of RAPD and ERIC |
| title | Typing of Ralstonia solanacearum isolated from tomato by antibiotic susceptibility, plasmid profiling and PCR-based techniques of RAPD and ERIC |
| title_full | Typing of Ralstonia solanacearum isolated from tomato by antibiotic susceptibility, plasmid profiling and PCR-based techniques of RAPD and ERIC |
| title_fullStr | Typing of Ralstonia solanacearum isolated from tomato by antibiotic susceptibility, plasmid profiling and PCR-based techniques of RAPD and ERIC |
| title_full_unstemmed | Typing of Ralstonia solanacearum isolated from tomato by antibiotic susceptibility, plasmid profiling and PCR-based techniques of RAPD and ERIC |
| title_short | Typing of Ralstonia solanacearum isolated from tomato by antibiotic susceptibility, plasmid profiling and PCR-based techniques of RAPD and ERIC |
| title_sort | typing of ralstonia solanacearum isolated from tomato by antibiotic susceptibility plasmid profiling and pcr based techniques of rapd and eric |
| url | http://psasir.upm.edu.my/id/eprint/48936/1/Typing%20of%20Ralstonia%20solanacearum%20isolated%20from%20tomato%20by%20antibiotic%20susceptibility%2C%20plasmid%20profiling%20and%20PCR-based%20techniques%20of%20RAPD%20and%20ERIC.pdf |
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