Substrate specificity of lipases from four species of Aspergillus towards hydrolysis of homoacid triacylglycerols and vegetable oils in non-aqueous system
Among the four species of Aspergillus, Aspergillus oryzea lipase demonstrates high preference towards medium chain triacylglycerols (C10) and discriminates against triunsaturated triacylglycerol (TAG) e.g. triolein. The great discriminating power of its lipase against triolein was shown in compariso...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Malaysian Agricultural Research and Development Institute (MARDI)
2006
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/48944/1/Substrate%20specificity%20of%20lipases%20from%20four%20species%20of%20Aspergillus%20towards%20hydrolysis%20of%20homoacid%20triacylglycerols%20and%20vegetable%20oils%20in%20non-aqueous%20system.pdf |
| _version_ | 1825930104652431360 |
|---|---|
| author | Long, Kamariah Hashim, Nurul Huda Mohd Danial, Azlina Osman, Rosmawati Mohd Ghazali, Hasanah Lai, Oi Ming Hamzah, Ainon |
| author_facet | Long, Kamariah Hashim, Nurul Huda Mohd Danial, Azlina Osman, Rosmawati Mohd Ghazali, Hasanah Lai, Oi Ming Hamzah, Ainon |
| author_sort | Long, Kamariah |
| collection | UPM |
| description | Among the four species of Aspergillus, Aspergillus oryzea lipase demonstrates high preference towards medium chain triacylglycerols (C10) and discriminates against triunsaturated triacylglycerol (TAG) e.g. triolein. The great discriminating power of its lipase against triolein was shown in comparison with its ability to catalyse the hydrolysis of medium chain (C10) TAG e.g. tricaprin and less shown when hydrolysing saturated long chain TAG i.e. tripalmitin. The discriminating power of these lipases are in this order: triolein > tripalmitin > trilaurin > tricaprin. Similar phenomenon was noted when mycelium-bound lipases of Aspergillus sp. were used to catalyse the hydrolysis of coconut oil, palm olein, olive oil and flaxseed oil. In most cases, relative percentage of monounsaturated fatty acid (C18:1) in non-hydrolysed fractions of oils increased after 12 days of hydrolysis. Hydrolysis of flaxseed and olive oil showed that Aspergillus murarum, Aspergillus oryzea and Aspergillus flavus lipases have high preference towards polyunsaturated fatty acids i.e. linoleic acid (C18:2) in olive oil and linolenic acid (C18:3) in flaxseed oil and no preference for monounsaturated and saturated long chain fatty acids. |
| first_indexed | 2024-03-06T09:06:33Z |
| format | Article |
| id | upm.eprints-48944 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:06:33Z |
| publishDate | 2006 |
| publisher | Malaysian Agricultural Research and Development Institute (MARDI) |
| record_format | dspace |
| spelling | upm.eprints-489442016-12-30T02:25:59Z http://psasir.upm.edu.my/id/eprint/48944/ Substrate specificity of lipases from four species of Aspergillus towards hydrolysis of homoacid triacylglycerols and vegetable oils in non-aqueous system Long, Kamariah Hashim, Nurul Huda Mohd Danial, Azlina Osman, Rosmawati Mohd Ghazali, Hasanah Lai, Oi Ming Hamzah, Ainon Among the four species of Aspergillus, Aspergillus oryzea lipase demonstrates high preference towards medium chain triacylglycerols (C10) and discriminates against triunsaturated triacylglycerol (TAG) e.g. triolein. The great discriminating power of its lipase against triolein was shown in comparison with its ability to catalyse the hydrolysis of medium chain (C10) TAG e.g. tricaprin and less shown when hydrolysing saturated long chain TAG i.e. tripalmitin. The discriminating power of these lipases are in this order: triolein > tripalmitin > trilaurin > tricaprin. Similar phenomenon was noted when mycelium-bound lipases of Aspergillus sp. were used to catalyse the hydrolysis of coconut oil, palm olein, olive oil and flaxseed oil. In most cases, relative percentage of monounsaturated fatty acid (C18:1) in non-hydrolysed fractions of oils increased after 12 days of hydrolysis. Hydrolysis of flaxseed and olive oil showed that Aspergillus murarum, Aspergillus oryzea and Aspergillus flavus lipases have high preference towards polyunsaturated fatty acids i.e. linoleic acid (C18:2) in olive oil and linolenic acid (C18:3) in flaxseed oil and no preference for monounsaturated and saturated long chain fatty acids. Malaysian Agricultural Research and Development Institute (MARDI) 2006 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/48944/1/Substrate%20specificity%20of%20lipases%20from%20four%20species%20of%20Aspergillus%20towards%20hydrolysis%20of%20homoacid%20triacylglycerols%20and%20vegetable%20oils%20in%20non-aqueous%20system.pdf Long, Kamariah and Hashim, Nurul Huda and Mohd Danial, Azlina and Osman, Rosmawati and Mohd Ghazali, Hasanah and Lai, Oi Ming and Hamzah, Ainon (2006) Substrate specificity of lipases from four species of Aspergillus towards hydrolysis of homoacid triacylglycerols and vegetable oils in non-aqueous system. Journal of Tropical Agriculture and Food Science, 34 (1). pp. 103-109. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/41-2006/volume-34-no1/181-340111 |
| spellingShingle | Long, Kamariah Hashim, Nurul Huda Mohd Danial, Azlina Osman, Rosmawati Mohd Ghazali, Hasanah Lai, Oi Ming Hamzah, Ainon Substrate specificity of lipases from four species of Aspergillus towards hydrolysis of homoacid triacylglycerols and vegetable oils in non-aqueous system |
| title | Substrate specificity of lipases from four species of Aspergillus towards hydrolysis of homoacid triacylglycerols and vegetable oils in non-aqueous system |
| title_full | Substrate specificity of lipases from four species of Aspergillus towards hydrolysis of homoacid triacylglycerols and vegetable oils in non-aqueous system |
| title_fullStr | Substrate specificity of lipases from four species of Aspergillus towards hydrolysis of homoacid triacylglycerols and vegetable oils in non-aqueous system |
| title_full_unstemmed | Substrate specificity of lipases from four species of Aspergillus towards hydrolysis of homoacid triacylglycerols and vegetable oils in non-aqueous system |
| title_short | Substrate specificity of lipases from four species of Aspergillus towards hydrolysis of homoacid triacylglycerols and vegetable oils in non-aqueous system |
| title_sort | substrate specificity of lipases from four species of aspergillus towards hydrolysis of homoacid triacylglycerols and vegetable oils in non aqueous system |
| url | http://psasir.upm.edu.my/id/eprint/48944/1/Substrate%20specificity%20of%20lipases%20from%20four%20species%20of%20Aspergillus%20towards%20hydrolysis%20of%20homoacid%20triacylglycerols%20and%20vegetable%20oils%20in%20non-aqueous%20system.pdf |
| work_keys_str_mv | AT longkamariah substratespecificityoflipasesfromfourspeciesofaspergillustowardshydrolysisofhomoacidtriacylglycerolsandvegetableoilsinnonaqueoussystem AT hashimnurulhuda substratespecificityoflipasesfromfourspeciesofaspergillustowardshydrolysisofhomoacidtriacylglycerolsandvegetableoilsinnonaqueoussystem AT mohddanialazlina substratespecificityoflipasesfromfourspeciesofaspergillustowardshydrolysisofhomoacidtriacylglycerolsandvegetableoilsinnonaqueoussystem AT osmanrosmawati substratespecificityoflipasesfromfourspeciesofaspergillustowardshydrolysisofhomoacidtriacylglycerolsandvegetableoilsinnonaqueoussystem AT mohdghazalihasanah substratespecificityoflipasesfromfourspeciesofaspergillustowardshydrolysisofhomoacidtriacylglycerolsandvegetableoilsinnonaqueoussystem AT laioiming substratespecificityoflipasesfromfourspeciesofaspergillustowardshydrolysisofhomoacidtriacylglycerolsandvegetableoilsinnonaqueoussystem AT hamzahainon substratespecificityoflipasesfromfourspeciesofaspergillustowardshydrolysisofhomoacidtriacylglycerolsandvegetableoilsinnonaqueoussystem |