Aeration and agitation strategies for the improvement of red pigment production by Monascus purpureus FTC 5391
The influence of agitation speed and aeration rate on mycelial morphology and red pigment production of Monascus purpureus FTC 5391 was investigated in 2-litre stirred tank bioreactor. At agitation speed of 400 rpm or less, serious mycelia aggregation and fluffy pellets were present with pigment yie...
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| Format: | Article |
| Language: | English |
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Malaysian Agricultural Research and Development Institute (MARDI)
2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/48945/1/Aeration%20and%20agitation%20strategies%20for%20the%20improvement%20of%20red%20pigment%20production%20by%20Monascus%20purpureus%20FTC%205391.pdf |
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| author | Abdul Manan, Musaalbakri Ariff, Arbakariya Mohamad, Rosfarizan Abdul Karim, Mohamed Ismail |
| author_facet | Abdul Manan, Musaalbakri Ariff, Arbakariya Mohamad, Rosfarizan Abdul Karim, Mohamed Ismail |
| author_sort | Abdul Manan, Musaalbakri |
| collection | UPM |
| description | The influence of agitation speed and aeration rate on mycelial morphology and red pigment production of Monascus purpureus FTC 5391 was investigated in 2-litre stirred tank bioreactor. At agitation speed of 400 rpm or less, serious mycelia aggregation and fluffy pellets were present with pigment yields of approximately below 0.27 UA500/g.litre. At this speed, M. purpureus FTC 5391 formed long hairy mycelia, resulting in the formation of viscous cultures. As the agitation speed increased from 200 to 1,000 rpm, the KLa value also increased gradually. The specific oxygen uptake rates (QO2) at 800 and 1,000 rpm were lower than the rate at 600 rpm. Mycelia had short branches and appeared to be damaged by mechanical shear forces due to high agitation speed. The dissolved oxygen tension (DOT) levels were varied within the ranges of 30 –100% saturation in fermentation with agitation speed fixed at 600 rpm. The maximum cell concentration of M. purpureus FTC 5391 was about 13.2 g/litre and yield of red pigment production of about 0.411 UA500/g.litre was achieved with DOT of 100% saturation. The highly branched cell morphology and vacuolated cell morphology were observed in a high DOT level (100% saturation). Fermentation was successfully developed in 2-litre stirred tank bioreactor based on a constant agitation speed of 600 rpm and DOT levels higher than 90% saturation, which was the best condition for pigment production and cell morphology. |
| first_indexed | 2024-03-06T09:06:33Z |
| format | Article |
| id | upm.eprints-48945 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:06:33Z |
| publishDate | 2006 |
| publisher | Malaysian Agricultural Research and Development Institute (MARDI) |
| record_format | dspace |
| spelling | upm.eprints-489452016-12-30T02:26:01Z http://psasir.upm.edu.my/id/eprint/48945/ Aeration and agitation strategies for the improvement of red pigment production by Monascus purpureus FTC 5391 Abdul Manan, Musaalbakri Ariff, Arbakariya Mohamad, Rosfarizan Abdul Karim, Mohamed Ismail The influence of agitation speed and aeration rate on mycelial morphology and red pigment production of Monascus purpureus FTC 5391 was investigated in 2-litre stirred tank bioreactor. At agitation speed of 400 rpm or less, serious mycelia aggregation and fluffy pellets were present with pigment yields of approximately below 0.27 UA500/g.litre. At this speed, M. purpureus FTC 5391 formed long hairy mycelia, resulting in the formation of viscous cultures. As the agitation speed increased from 200 to 1,000 rpm, the KLa value also increased gradually. The specific oxygen uptake rates (QO2) at 800 and 1,000 rpm were lower than the rate at 600 rpm. Mycelia had short branches and appeared to be damaged by mechanical shear forces due to high agitation speed. The dissolved oxygen tension (DOT) levels were varied within the ranges of 30 –100% saturation in fermentation with agitation speed fixed at 600 rpm. The maximum cell concentration of M. purpureus FTC 5391 was about 13.2 g/litre and yield of red pigment production of about 0.411 UA500/g.litre was achieved with DOT of 100% saturation. The highly branched cell morphology and vacuolated cell morphology were observed in a high DOT level (100% saturation). Fermentation was successfully developed in 2-litre stirred tank bioreactor based on a constant agitation speed of 600 rpm and DOT levels higher than 90% saturation, which was the best condition for pigment production and cell morphology. Malaysian Agricultural Research and Development Institute (MARDI) 2006 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/48945/1/Aeration%20and%20agitation%20strategies%20for%20the%20improvement%20of%20red%20pigment%20production%20by%20Monascus%20purpureus%20FTC%205391.pdf Abdul Manan, Musaalbakri and Ariff, Arbakariya and Mohamad, Rosfarizan and Abdul Karim, Mohamed Ismail (2006) Aeration and agitation strategies for the improvement of red pigment production by Monascus purpureus FTC 5391. Journal of Tropical Agriculture and Food Science, 34 (1). pp. 89-102. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/41-2006/volume-34-no1/180-340110 |
| spellingShingle | Abdul Manan, Musaalbakri Ariff, Arbakariya Mohamad, Rosfarizan Abdul Karim, Mohamed Ismail Aeration and agitation strategies for the improvement of red pigment production by Monascus purpureus FTC 5391 |
| title | Aeration and agitation strategies for the improvement of red pigment production by Monascus purpureus FTC 5391 |
| title_full | Aeration and agitation strategies for the improvement of red pigment production by Monascus purpureus FTC 5391 |
| title_fullStr | Aeration and agitation strategies for the improvement of red pigment production by Monascus purpureus FTC 5391 |
| title_full_unstemmed | Aeration and agitation strategies for the improvement of red pigment production by Monascus purpureus FTC 5391 |
| title_short | Aeration and agitation strategies for the improvement of red pigment production by Monascus purpureus FTC 5391 |
| title_sort | aeration and agitation strategies for the improvement of red pigment production by monascus purpureus ftc 5391 |
| url | http://psasir.upm.edu.my/id/eprint/48945/1/Aeration%20and%20agitation%20strategies%20for%20the%20improvement%20of%20red%20pigment%20production%20by%20Monascus%20purpureus%20FTC%205391.pdf |
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