Real-time PCR for detection of fliC gene of E. coli O157:H7 in beef and chicken meat
The SYBR Green I real-time PCR assay was used to quantify E. coli O157:H7 in various meat samples. Primers were designed to amplify and quantify the structural flagella (fliC) gene of E. coli O157:H7 in a single reaction. The primer specificity was confirmed with DNA from an ATCC culture of E. coli...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Malaysian Agricultural Research and Development Institute (MARDI)
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/49301/1/Real-time%20PCR%20for%20detection%20of%20fliC%20gene%20of%20E.%20coli%20O157H7%20in%20beef%20and%20chicken%20meat.pdf |