Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species
The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus n...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/50079/1/Newly%20developed%20microsatellite%20markers%20of%20Mystus%20nemurus%20tested%20for%20cross-species%20amplification%20in%20two%20distantly%20related%20aquacultured%20catfish%20species.pdf |
| _version_ | 1796975259095662592 |
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| author | Chan, Soon Choy Tan, Soon Guan Siraj, Siti Shapor Mohd Yusoff, Khatijah |
| author_facet | Chan, Soon Choy Tan, Soon Guan Siraj, Siti Shapor Mohd Yusoff, Khatijah |
| author_sort | Chan, Soon Choy |
| collection | UPM |
| description | The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species. |
| first_indexed | 2024-03-06T09:08:54Z |
| format | Article |
| id | upm.eprints-50079 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:08:54Z |
| publishDate | 2005 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | dspace |
| spelling | upm.eprints-500792017-02-01T04:37:31Z http://psasir.upm.edu.my/id/eprint/50079/ Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species Chan, Soon Choy Tan, Soon Guan Siraj, Siti Shapor Mohd Yusoff, Khatijah The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species. Asian-Australasian Association of Animal Production Societies 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50079/1/Newly%20developed%20microsatellite%20markers%20of%20Mystus%20nemurus%20tested%20for%20cross-species%20amplification%20in%20two%20distantly%20related%20aquacultured%20catfish%20species.pdf Chan, Soon Choy and Tan, Soon Guan and Siraj, Siti Shapor and Mohd Yusoff, Khatijah (2005) Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species. Asian-Australasian Journal of Animal Sciences, 18 (11). pp. 1513-1518. ISSN 1011-2367; ESSN: 1976-5517 http://www.ajas.info/journal/view.php?number=21149 10.5713/ajas.2005.1513 |
| spellingShingle | Chan, Soon Choy Tan, Soon Guan Siraj, Siti Shapor Mohd Yusoff, Khatijah Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species |
| title | Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species |
| title_full | Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species |
| title_fullStr | Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species |
| title_full_unstemmed | Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species |
| title_short | Newly developed microsatellite markers of Mystus nemurus tested for cross-species amplification in two distantly related aquacultured catfish species |
| title_sort | newly developed microsatellite markers of mystus nemurus tested for cross species amplification in two distantly related aquacultured catfish species |
| url | http://psasir.upm.edu.my/id/eprint/50079/1/Newly%20developed%20microsatellite%20markers%20of%20Mystus%20nemurus%20tested%20for%20cross-species%20amplification%20in%20two%20distantly%20related%20aquacultured%20catfish%20species.pdf |
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