CELLISA: a rapid and reliable method for primary screening of hybridoma supernatant for cell surface antigens
Cell surface markers are particularly important in immunology to identify and differentiate the many different types and stages of cells that respond to foreign intervention including infecting pathogens. These are especially lacking in the field of veterinary science. Specific monoclonal antibodies...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/50204/1/2-1.pdf |
| _version_ | 1825930286798471168 |
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| author | Chong, Chou Min Than, Leslie Thian Lung Shamsudin, Mariana Nor Md. Yusoff, Fatimah Abdullah, Maha |
| author_facet | Chong, Chou Min Than, Leslie Thian Lung Shamsudin, Mariana Nor Md. Yusoff, Fatimah Abdullah, Maha |
| author_sort | Chong, Chou Min |
| collection | UPM |
| description | Cell surface markers are particularly important in immunology to identify and differentiate the many different types and stages of cells that respond to foreign intervention including infecting pathogens. These are especially lacking in the field of veterinary science. Specific monoclonal antibodies are produced via the
hybridoma technology. Rapid and sensitive assays are crucial in preliminary screening as early hybridoma clones only secrete low amounts of antibodies. A cell-based enzyme-linked immunosorbent assay (CELLISA) is described for the primary screening of hybridoma supernatants. Time is saved as whole cell suspensions of lymphocyte, adherent cell like macrophages and fragile cells like erythrocytes and granulocytes are directly coated, without modification, to plates. The methodology is adaptable to be employed to a variety of different cell types with no or little alteration of surface antigens. |
| first_indexed | 2024-03-06T09:09:17Z |
| format | Conference or Workshop Item |
| id | upm.eprints-50204 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:09:17Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | upm.eprints-502042017-02-02T04:44:54Z http://psasir.upm.edu.my/id/eprint/50204/ CELLISA: a rapid and reliable method for primary screening of hybridoma supernatant for cell surface antigens Chong, Chou Min Than, Leslie Thian Lung Shamsudin, Mariana Nor Md. Yusoff, Fatimah Abdullah, Maha Cell surface markers are particularly important in immunology to identify and differentiate the many different types and stages of cells that respond to foreign intervention including infecting pathogens. These are especially lacking in the field of veterinary science. Specific monoclonal antibodies are produced via the hybridoma technology. Rapid and sensitive assays are crucial in preliminary screening as early hybridoma clones only secrete low amounts of antibodies. A cell-based enzyme-linked immunosorbent assay (CELLISA) is described for the primary screening of hybridoma supernatants. Time is saved as whole cell suspensions of lymphocyte, adherent cell like macrophages and fragile cells like erythrocytes and granulocytes are directly coated, without modification, to plates. The methodology is adaptable to be employed to a variety of different cell types with no or little alteration of surface antigens. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50204/1/2-1.pdf Chong, Chou Min and Than, Leslie Thian Lung and Shamsudin, Mariana Nor and Md. Yusoff, Fatimah and Abdullah, Maha (2012) CELLISA: a rapid and reliable method for primary screening of hybridoma supernatant for cell surface antigens. In: International Conference on One Health and 24th VAM Congress 2012, 21-23 Sept. 2012, Marriott Putrajaya. (pp. 291-292). |
| spellingShingle | Chong, Chou Min Than, Leslie Thian Lung Shamsudin, Mariana Nor Md. Yusoff, Fatimah Abdullah, Maha CELLISA: a rapid and reliable method for primary screening of hybridoma supernatant for cell surface antigens |
| title | CELLISA: a rapid and reliable method for primary screening of hybridoma supernatant for cell surface antigens |
| title_full | CELLISA: a rapid and reliable method for primary screening of hybridoma supernatant for cell surface antigens |
| title_fullStr | CELLISA: a rapid and reliable method for primary screening of hybridoma supernatant for cell surface antigens |
| title_full_unstemmed | CELLISA: a rapid and reliable method for primary screening of hybridoma supernatant for cell surface antigens |
| title_short | CELLISA: a rapid and reliable method for primary screening of hybridoma supernatant for cell surface antigens |
| title_sort | cellisa a rapid and reliable method for primary screening of hybridoma supernatant for cell surface antigens |
| url | http://psasir.upm.edu.my/id/eprint/50204/1/2-1.pdf |
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