Conjugal transfer of plasmids and antibiotic resistance among Escherichia coli isolated from animals in a rural area in Sarawak (Malaysia)
Thirty-six strains of Escherichia coli isolated from animals in Bario, a remote area in Sarawak, Malaysia, were examined for presence of plasmid DNA and their susceptibility to nine antimicrobial agents. Of the total 36 isolates, five bovine and six canine isolates were found to contain plasmid DNA...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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The Society for Applied Bacteriology
1997
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51106/1/51106.pdf |
| _version_ | 1825930466681683968 |
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| author | Radu, Son Rahmat Ali, Gulam Rusul Abdul Karim, Mohamed Ismail |
| author_facet | Radu, Son Rahmat Ali, Gulam Rusul Abdul Karim, Mohamed Ismail |
| author_sort | Radu, Son |
| collection | UPM |
| description | Thirty-six strains of Escherichia coli isolated from animals in Bario, a remote area in Sarawak, Malaysia, were examined for presence of plasmid DNA and their susceptibility to nine antimicrobial agents. Of the total 36 isolates, five bovine and six canine isolates were found to contain plasmid DNA ranging in sizes from 2.6 to 70 kilobases. All were susceptible to chloramphenicol, erythromycin, gentamicin, nalidixic acid and neomycin but resistance to ampicillin (47%), erythromycin (19%), streptomycin (25%) and tetracycline (11%) was observed. Resistance was associated with carriage of a 47 kb (SC98), 70 kb, (SC133) and 56 and 46 kb (SC119) plasmids which were transmissible to the Escherichia coli K12 recipient. It is concluded that animals form a potential reservoir of R plasmids carrying E. coli in the study area. |
| first_indexed | 2024-03-06T09:11:58Z |
| format | Article |
| id | upm.eprints-51106 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:11:58Z |
| publishDate | 1997 |
| publisher | The Society for Applied Bacteriology |
| record_format | dspace |
| spelling | upm.eprints-511062017-04-27T09:52:28Z http://psasir.upm.edu.my/id/eprint/51106/ Conjugal transfer of plasmids and antibiotic resistance among Escherichia coli isolated from animals in a rural area in Sarawak (Malaysia) Radu, Son Rahmat Ali, Gulam Rusul Abdul Karim, Mohamed Ismail Thirty-six strains of Escherichia coli isolated from animals in Bario, a remote area in Sarawak, Malaysia, were examined for presence of plasmid DNA and their susceptibility to nine antimicrobial agents. Of the total 36 isolates, five bovine and six canine isolates were found to contain plasmid DNA ranging in sizes from 2.6 to 70 kilobases. All were susceptible to chloramphenicol, erythromycin, gentamicin, nalidixic acid and neomycin but resistance to ampicillin (47%), erythromycin (19%), streptomycin (25%) and tetracycline (11%) was observed. Resistance was associated with carriage of a 47 kb (SC98), 70 kb, (SC133) and 56 and 46 kb (SC119) plasmids which were transmissible to the Escherichia coli K12 recipient. It is concluded that animals form a potential reservoir of R plasmids carrying E. coli in the study area. The Society for Applied Bacteriology 1997 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51106/1/51106.pdf Radu, Son and Rahmat Ali, Gulam Rusul and Abdul Karim, Mohamed Ismail (1997) Conjugal transfer of plasmids and antibiotic resistance among Escherichia coli isolated from animals in a rural area in Sarawak (Malaysia). Journal of Applied Microbiology, 82 (2). pp. 240-244. ISSN 1364-5072; ESSN: 1365-2672 http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.1997.tb03579.x/abstract 10.1111/j.1365-2672.1997.tb03579.x |
| spellingShingle | Radu, Son Rahmat Ali, Gulam Rusul Abdul Karim, Mohamed Ismail Conjugal transfer of plasmids and antibiotic resistance among Escherichia coli isolated from animals in a rural area in Sarawak (Malaysia) |
| title | Conjugal transfer of plasmids and antibiotic resistance among Escherichia coli isolated from animals in a rural area in Sarawak (Malaysia) |
| title_full | Conjugal transfer of plasmids and antibiotic resistance among Escherichia coli isolated from animals in a rural area in Sarawak (Malaysia) |
| title_fullStr | Conjugal transfer of plasmids and antibiotic resistance among Escherichia coli isolated from animals in a rural area in Sarawak (Malaysia) |
| title_full_unstemmed | Conjugal transfer of plasmids and antibiotic resistance among Escherichia coli isolated from animals in a rural area in Sarawak (Malaysia) |
| title_short | Conjugal transfer of plasmids and antibiotic resistance among Escherichia coli isolated from animals in a rural area in Sarawak (Malaysia) |
| title_sort | conjugal transfer of plasmids and antibiotic resistance among escherichia coli isolated from animals in a rural area in sarawak malaysia |
| url | http://psasir.upm.edu.my/id/eprint/51106/1/51106.pdf |
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