Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro
BACKGROUND: Condensed tannin (CT) fractions of different molecular weights (MWs) may affect rumen microbial metabolism by altering bacterial diversity. In this study the effects of unfractionated CTs (F0) and five CT fractions (F1–F5) of different MWs (F1, 1265.8 Da; F2, 1028.6 Da; F3, 652.2 Da; F4,...
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| Format: | Article |
| Language: | English |
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Society of Chemical Industry
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51174/1/Modulatory%20effects%20of%20condensed%20tannin%20fractions%20of%20different%20molecular%20weights%20from%20a%20Leucaena%20leucocephala%20hybrid%20on%20the%20bovine%20rumen%20bacterial%20community%20in%20vitro.pdf |
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| author | Poothan Mookiah, Saminathan Sieo, Chin Chin Gan, Han Ming Ravi, Sharanya Venkatachalam, Karthikkumar Abdullah, Norhani Wong, Clemente Michael Vui Ling Ho, Yin Wan |
| author_facet | Poothan Mookiah, Saminathan Sieo, Chin Chin Gan, Han Ming Ravi, Sharanya Venkatachalam, Karthikkumar Abdullah, Norhani Wong, Clemente Michael Vui Ling Ho, Yin Wan |
| author_sort | Poothan Mookiah, Saminathan |
| collection | UPM |
| description | BACKGROUND: Condensed tannin (CT) fractions of different molecular weights (MWs) may affect rumen microbial metabolism by altering bacterial diversity. In this study the effects of unfractionated CTs (F0) and five CT fractions (F1–F5) of different MWs (F1, 1265.8 Da; F2, 1028.6 Da; F3, 652.2 Da; F4, 562.2 Da; F5, 469.6 Da) from Leucaena leucocephala hybrid-Rendang (LLR) on the structure and diversity of the rumen bacterial community were investigated in vitro. RESULTS: Real-time polymerase chain reaction assay showed that the total bacterial population was not significantly (P > 0.05) different among the dietary treatments. Inclusion of higher-MW CT fractions F1 and F2 significantly (P < 0.05) increased the Fibrobacter succinogenes population compared with F0 and CT fractions F3–F5. Although inclusion of F0 and CT fractions (F1–F5) significantly (P < 0.05) decreased the Ruminococcus flavefaciens population, there was no effect on the Ruminococcus albus population when compared with the control (without CTs). High-throughput sequencing of the V3 region of 16S rRNA showed that the relative abundance of genera Prevotella and unclassified Clostridiales was significantly (P < 0.05) decreased, corresponding with increasing MW of CT fractions, whereas cellulolytic bacteria of the genus Fibrobacter were significantly (P < 0.05) increased. Inclusion of higher-MW CT fractions F1 and/or F2 decreased the relative abundance of minor genera such as Ruminococcus, Streptococcus, Clostridium XIVa and Anaeroplasma but increased the relative abundance of Acinetobacter, Treponema, Selenomonas, Succiniclasticum and unclassified Spirochaetales compared with the control and lower-MW CT fractions. CONCLUSION: This study indicates that CT fractions of different MWs may play an important role in altering the structure and diversity of the rumen bacterial community in vitro, and the impact was more pronounced for CT fractions with higher MW. |
| first_indexed | 2024-03-06T09:12:09Z |
| format | Article |
| id | upm.eprints-51174 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:12:09Z |
| publishDate | 2016 |
| publisher | Society of Chemical Industry |
| record_format | dspace |
| spelling | upm.eprints-511742017-05-02T04:40:22Z http://psasir.upm.edu.my/id/eprint/51174/ Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro Poothan Mookiah, Saminathan Sieo, Chin Chin Gan, Han Ming Ravi, Sharanya Venkatachalam, Karthikkumar Abdullah, Norhani Wong, Clemente Michael Vui Ling Ho, Yin Wan BACKGROUND: Condensed tannin (CT) fractions of different molecular weights (MWs) may affect rumen microbial metabolism by altering bacterial diversity. In this study the effects of unfractionated CTs (F0) and five CT fractions (F1–F5) of different MWs (F1, 1265.8 Da; F2, 1028.6 Da; F3, 652.2 Da; F4, 562.2 Da; F5, 469.6 Da) from Leucaena leucocephala hybrid-Rendang (LLR) on the structure and diversity of the rumen bacterial community were investigated in vitro. RESULTS: Real-time polymerase chain reaction assay showed that the total bacterial population was not significantly (P > 0.05) different among the dietary treatments. Inclusion of higher-MW CT fractions F1 and F2 significantly (P < 0.05) increased the Fibrobacter succinogenes population compared with F0 and CT fractions F3–F5. Although inclusion of F0 and CT fractions (F1–F5) significantly (P < 0.05) decreased the Ruminococcus flavefaciens population, there was no effect on the Ruminococcus albus population when compared with the control (without CTs). High-throughput sequencing of the V3 region of 16S rRNA showed that the relative abundance of genera Prevotella and unclassified Clostridiales was significantly (P < 0.05) decreased, corresponding with increasing MW of CT fractions, whereas cellulolytic bacteria of the genus Fibrobacter were significantly (P < 0.05) increased. Inclusion of higher-MW CT fractions F1 and/or F2 decreased the relative abundance of minor genera such as Ruminococcus, Streptococcus, Clostridium XIVa and Anaeroplasma but increased the relative abundance of Acinetobacter, Treponema, Selenomonas, Succiniclasticum and unclassified Spirochaetales compared with the control and lower-MW CT fractions. CONCLUSION: This study indicates that CT fractions of different MWs may play an important role in altering the structure and diversity of the rumen bacterial community in vitro, and the impact was more pronounced for CT fractions with higher MW. Society of Chemical Industry 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51174/1/Modulatory%20effects%20of%20condensed%20tannin%20fractions%20of%20different%20molecular%20weights%20from%20a%20Leucaena%20leucocephala%20hybrid%20on%20the%20bovine%20rumen%20bacterial%20community%20in%20vitro.pdf Poothan Mookiah, Saminathan and Sieo, Chin Chin and Gan, Han Ming and Ravi, Sharanya and Venkatachalam, Karthikkumar and Abdullah, Norhani and Wong, Clemente Michael Vui Ling and Ho, Yin Wan (2016) Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro. Journal of the Science of Food and Agriculture, 96 (13). pp. 4565-4574. ISSN 0022-5142; ESSN: 1097-0010 http://onlinelibrary.wiley.com/wol1/doi/10.1002/jsfa.7674/abstract 10.1002/jsfa.7674 |
| spellingShingle | Poothan Mookiah, Saminathan Sieo, Chin Chin Gan, Han Ming Ravi, Sharanya Venkatachalam, Karthikkumar Abdullah, Norhani Wong, Clemente Michael Vui Ling Ho, Yin Wan Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro |
| title | Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro |
| title_full | Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro |
| title_fullStr | Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro |
| title_full_unstemmed | Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro |
| title_short | Modulatory effects of condensed tannin fractions of different molecular weights from a Leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro |
| title_sort | modulatory effects of condensed tannin fractions of different molecular weights from a leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro |
| url | http://psasir.upm.edu.my/id/eprint/51174/1/Modulatory%20effects%20of%20condensed%20tannin%20fractions%20of%20different%20molecular%20weights%20from%20a%20Leucaena%20leucocephala%20hybrid%20on%20the%20bovine%20rumen%20bacterial%20community%20in%20vitro.pdf |
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