Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis
The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Pharmacognosy
1998
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51340/1/51340.pdf |
| _version_ | 1796975515025801216 |
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| author | Ali, Abdul Manaf Ismail, Intan Safinar Mohamed Mackeen, Muhammad Mukram El-Sharkawy, Saleh Hassan Takahata, Kyoya Kanzaki, Hiroshi Kawazu, Kazuyoshi |
| author_facet | Ali, Abdul Manaf Ismail, Intan Safinar Mohamed Mackeen, Muhammad Mukram El-Sharkawy, Saleh Hassan Takahata, Kyoya Kanzaki, Hiroshi Kawazu, Kazuyoshi |
| author_sort | Ali, Abdul Manaf |
| collection | UPM |
| description | The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines tested (KU8112F-chronic mylogeneous leukemia, TK 10-renal carcinoma, UACC 62-melanoma and CEM-SS - T-lymphoblastic leukemia) were found to be susceptible to deoxypodophyllotoxin, however, the minimum effective concentration (MEC) required to reduce the cell population by 100 percent was different between cell lines. |
| first_indexed | 2024-03-06T09:12:39Z |
| format | Article |
| id | upm.eprints-51340 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:12:39Z |
| publishDate | 1998 |
| publisher | Korean Society of Pharmacognosy |
| record_format | dspace |
| spelling | upm.eprints-513402017-07-04T03:56:37Z http://psasir.upm.edu.my/id/eprint/51340/ Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis Ali, Abdul Manaf Ismail, Intan Safinar Mohamed Mackeen, Muhammad Mukram El-Sharkawy, Saleh Hassan Takahata, Kyoya Kanzaki, Hiroshi Kawazu, Kazuyoshi The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines tested (KU8112F-chronic mylogeneous leukemia, TK 10-renal carcinoma, UACC 62-melanoma and CEM-SS - T-lymphoblastic leukemia) were found to be susceptible to deoxypodophyllotoxin, however, the minimum effective concentration (MEC) required to reduce the cell population by 100 percent was different between cell lines. Korean Society of Pharmacognosy 1998 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51340/1/51340.pdf Ali, Abdul Manaf and Ismail, Intan Safinar and Mohamed Mackeen, Muhammad Mukram and El-Sharkawy, Saleh Hassan and Takahata, Kyoya and Kanzaki, Hiroshi and Kawazu, Kazuyoshi (1998) Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis. Natural Product Sciences, 4 (3). pp. 180-183. ISSN 1226-3907 http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=E1HSBY_1998_v4n3_180 |
| spellingShingle | Ali, Abdul Manaf Ismail, Intan Safinar Mohamed Mackeen, Muhammad Mukram El-Sharkawy, Saleh Hassan Takahata, Kyoya Kanzaki, Hiroshi Kawazu, Kazuyoshi Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis |
| title | Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis |
| title_full | Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis |
| title_fullStr | Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis |
| title_full_unstemmed | Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis |
| title_short | Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis |
| title_sort | bioassay guided isolation of deoxypodophyllotoxin the cytotoxic constituent of juniperus chinensis |
| url | http://psasir.upm.edu.my/id/eprint/51340/1/51340.pdf |
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