Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa)
A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3 kb that enabled the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Rapid Science Publishers
1998
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51780/1/51780.pdf http://psasir.upm.edu.my/id/eprint/51780/7/A_1008841514368.pdf |
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| author | R., Son Nasreldin, E. H. H., Zaiton L., Samuel Rusul, G. Nimita, F. |
| author_facet | R., Son Nasreldin, E. H. H., Zaiton L., Samuel Rusul, G. Nimita, F. |
| author_sort | R., Son |
| collection | UPM |
| description | A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3 kb that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The three primers generated polymorphisms in all 35 strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9 kb. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. |
| first_indexed | 2024-03-06T09:13:57Z |
| format | Article |
| id | upm.eprints-51780 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-09-25T03:35:06Z |
| publishDate | 1998 |
| publisher | Rapid Science Publishers |
| record_format | dspace |
| spelling | upm.eprints-517802024-08-08T00:08:02Z http://psasir.upm.edu.my/id/eprint/51780/ Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) R., Son Nasreldin, E. H. H., Zaiton L., Samuel Rusul, G. Nimita, F. A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3 kb that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The three primers generated polymorphisms in all 35 strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9 kb. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. Rapid Science Publishers 1998 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/51780/1/51780.pdf text en http://psasir.upm.edu.my/id/eprint/51780/7/A_1008841514368.pdf R., Son and Nasreldin, E. H. and H., Zaiton and L., Samuel and Rusul, G. and Nimita, F. (1998) Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa). World Journal of Microbiology and Biotechnology, 14 (6). pp. 895-901. ISSN 0959-3993; ESSN: 1573-0972 https://rd.springer.com/article/10.1023/A:1008841514368 10.1023/A:1008841514368 |
| spellingShingle | R., Son Nasreldin, E. H. H., Zaiton L., Samuel Rusul, G. Nimita, F. Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
| title | Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
| title_full | Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
| title_fullStr | Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
| title_full_unstemmed | Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
| title_short | Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) |
| title_sort | use of randomly amplified polymorphic dna analysis to differentiate isolates of vibrio parahaemolyticus from cockles anadara granosa |
| url | http://psasir.upm.edu.my/id/eprint/51780/1/51780.pdf http://psasir.upm.edu.my/id/eprint/51780/7/A_1008841514368.pdf |
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