Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells
The yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) is widely used to determine cell viability in cell proliferation and cytotoxic assays. MTT is reduced by metabolically active cells to form an insoluble purple formazan product that is quantifiable by sp...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Sains Malaysia
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/51955/1/Reduction%20of%20MTT%20to%20purple%20formazan%20by%20vitamin%20E%20isomers%20in%20the%20absence%20of%20cells.pdf |
| _version_ | 1825930630510149632 |
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| author | Lim, Su Wen Loh, Hwei San Ting, Kang Nee Bradshaw, Tracey Dawn Allaudin, Zeenathul Nazariah |
| author_facet | Lim, Su Wen Loh, Hwei San Ting, Kang Nee Bradshaw, Tracey Dawn Allaudin, Zeenathul Nazariah |
| author_sort | Lim, Su Wen |
| collection | UPM |
| description | The yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) is widely used to determine cell viability in cell proliferation and cytotoxic assays. MTT is reduced by metabolically active cells to form an insoluble purple formazan product that is quantifiable by spectrophotometry. It is the most common and direct assay for cell viability. However, in this present study, we demonstrated that the vitamin E isomers α-β-γ-δ-tocotrienols and α-tocopherol were able to reduce MTT into a formazan product, despite the absence of living cells. For comparison, a second method for determining cell viability, which is the neutral red uptake assay, was used in parallel with the MTT assay. The results showed that neutral red did not interact with the vitamin E isomers. Our findings suggest that the MTT assay is not suitable for studying the proliferative effects of vitamin E isomers on cell growth. |
| first_indexed | 2024-03-06T09:14:29Z |
| format | Article |
| id | upm.eprints-51955 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:14:29Z |
| publishDate | 2015 |
| publisher | Penerbit Universiti Sains Malaysia |
| record_format | dspace |
| spelling | upm.eprints-519552017-05-04T04:29:54Z http://psasir.upm.edu.my/id/eprint/51955/ Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells Lim, Su Wen Loh, Hwei San Ting, Kang Nee Bradshaw, Tracey Dawn Allaudin, Zeenathul Nazariah The yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) is widely used to determine cell viability in cell proliferation and cytotoxic assays. MTT is reduced by metabolically active cells to form an insoluble purple formazan product that is quantifiable by spectrophotometry. It is the most common and direct assay for cell viability. However, in this present study, we demonstrated that the vitamin E isomers α-β-γ-δ-tocotrienols and α-tocopherol were able to reduce MTT into a formazan product, despite the absence of living cells. For comparison, a second method for determining cell viability, which is the neutral red uptake assay, was used in parallel with the MTT assay. The results showed that neutral red did not interact with the vitamin E isomers. Our findings suggest that the MTT assay is not suitable for studying the proliferative effects of vitamin E isomers on cell growth. Penerbit Universiti Sains Malaysia 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51955/1/Reduction%20of%20MTT%20to%20purple%20formazan%20by%20vitamin%20E%20isomers%20in%20the%20absence%20of%20cells.pdf Lim, Su Wen and Loh, Hwei San and Ting, Kang Nee and Bradshaw, Tracey Dawn and Allaudin, Zeenathul Nazariah (2015) Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells. Tropical Life Sciences Research, 26 (1). pp. 111-120. ISSN 1985-3718; ESSN: 2180-4249 http://www.tlsr.usm.my/TLSRvol26no1.html |
| spellingShingle | Lim, Su Wen Loh, Hwei San Ting, Kang Nee Bradshaw, Tracey Dawn Allaudin, Zeenathul Nazariah Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells |
| title | Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells |
| title_full | Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells |
| title_fullStr | Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells |
| title_full_unstemmed | Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells |
| title_short | Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells |
| title_sort | reduction of mtt to purple formazan by vitamin e isomers in the absence of cells |
| url | http://psasir.upm.edu.my/id/eprint/51955/1/Reduction%20of%20MTT%20to%20purple%20formazan%20by%20vitamin%20E%20isomers%20in%20the%20absence%20of%20cells.pdf |
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