Development of cellulose nanofibre (CNF) derived from kenaf bast fibre and it's potential in enzyme immobilization support
This research mainly focuses on developing a natural cellulose nanofibre (CNF) from kenaf bast fibre and its potential for enzyme immobilization support. CNF was isolated by using a combination between chemical and mechanical treatments such as alkaline process and high-intensity ultrasonication pro...
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| Format: | Article |
| Language: | English |
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Malaysian Analytical Sciences Society
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52028/1/Development%20of%20cellulose%20nanofibre%20%28CNF%29%20derived%20from%20kenaf%20bast%20fibre%20and%20it%27s%20potential%20in%20enzyme%20immobilization%20support.pdf |
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| author | Sulaiman, Safwan Mokhtar, Mohd Noriznan Naim, Mohd Nazli Samsu Baharuddin, Azhari Mohd Salleh, Mohamad Amran Sulaiman, Alawi |
| author_facet | Sulaiman, Safwan Mokhtar, Mohd Noriznan Naim, Mohd Nazli Samsu Baharuddin, Azhari Mohd Salleh, Mohamad Amran Sulaiman, Alawi |
| author_sort | Sulaiman, Safwan |
| collection | UPM |
| description | This research mainly focuses on developing a natural cellulose nanofibre (CNF) from kenaf bast fibre and its potential for enzyme immobilization support. CNF was isolated by using a combination between chemical and mechanical treatments such as alkaline process and high-intensity ultrasonication process to increase the efficiency of hemicellulose and lignin removal, and to reduce its size into nano-order. The morphological study was carried out by using scanning electron microscope (SEM), indicating most of CNF diameter in range of 50-90 nm was obtained. The result of chemical analysis shows that cellulose content of raw bast fibre, bleached pulp fibre and CNF are 66.4 %, 83.7 % and 90.0 %, respectively. By decreasing the size of cellulose fibre, it increases the number of (O–H) group on the surface that plays as important role in enzyme immobilization. Covalent immobilization of cyclodextrin glucanotransferase (CGTase) onto CNF support resulted in about 95.0 % of protein loading with 69.48 % of enzyme activity, indicating high immobilization yield of enzyme. The enzymatic reaction of immobilized CGTase was able to produce more than 40 % yield of α-CD. Reusability profile of immobilized CGTase resulted in more than 60 % of retained activity up to 7 cycles. Therefore, the CNF is highly potential to be applied as enzyme immobilization support. |
| first_indexed | 2024-03-06T09:14:42Z |
| format | Article |
| id | upm.eprints-52028 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:14:42Z |
| publishDate | 2016 |
| publisher | Malaysian Analytical Sciences Society |
| record_format | dspace |
| spelling | upm.eprints-520282017-05-04T09:06:38Z http://psasir.upm.edu.my/id/eprint/52028/ Development of cellulose nanofibre (CNF) derived from kenaf bast fibre and it's potential in enzyme immobilization support Sulaiman, Safwan Mokhtar, Mohd Noriznan Naim, Mohd Nazli Samsu Baharuddin, Azhari Mohd Salleh, Mohamad Amran Sulaiman, Alawi This research mainly focuses on developing a natural cellulose nanofibre (CNF) from kenaf bast fibre and its potential for enzyme immobilization support. CNF was isolated by using a combination between chemical and mechanical treatments such as alkaline process and high-intensity ultrasonication process to increase the efficiency of hemicellulose and lignin removal, and to reduce its size into nano-order. The morphological study was carried out by using scanning electron microscope (SEM), indicating most of CNF diameter in range of 50-90 nm was obtained. The result of chemical analysis shows that cellulose content of raw bast fibre, bleached pulp fibre and CNF are 66.4 %, 83.7 % and 90.0 %, respectively. By decreasing the size of cellulose fibre, it increases the number of (O–H) group on the surface that plays as important role in enzyme immobilization. Covalent immobilization of cyclodextrin glucanotransferase (CGTase) onto CNF support resulted in about 95.0 % of protein loading with 69.48 % of enzyme activity, indicating high immobilization yield of enzyme. The enzymatic reaction of immobilized CGTase was able to produce more than 40 % yield of α-CD. Reusability profile of immobilized CGTase resulted in more than 60 % of retained activity up to 7 cycles. Therefore, the CNF is highly potential to be applied as enzyme immobilization support. Malaysian Analytical Sciences Society 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52028/1/Development%20of%20cellulose%20nanofibre%20%28CNF%29%20derived%20from%20kenaf%20bast%20fibre%20and%20it%27s%20potential%20in%20enzyme%20immobilization%20support.pdf Sulaiman, Safwan and Mokhtar, Mohd Noriznan and Naim, Mohd Nazli and Samsu Baharuddin, Azhari and Mohd Salleh, Mohamad Amran and Sulaiman, Alawi (2016) Development of cellulose nanofibre (CNF) derived from kenaf bast fibre and it's potential in enzyme immobilization support. Malaysian Journal of Analytical Sciences, 20 (2). pp. 309-317. ISSN 1394-2506 http://www.ukm.my/mjas/mjas2017/ |
| spellingShingle | Sulaiman, Safwan Mokhtar, Mohd Noriznan Naim, Mohd Nazli Samsu Baharuddin, Azhari Mohd Salleh, Mohamad Amran Sulaiman, Alawi Development of cellulose nanofibre (CNF) derived from kenaf bast fibre and it's potential in enzyme immobilization support |
| title | Development of cellulose nanofibre (CNF) derived from kenaf bast fibre and it's potential in enzyme immobilization support |
| title_full | Development of cellulose nanofibre (CNF) derived from kenaf bast fibre and it's potential in enzyme immobilization support |
| title_fullStr | Development of cellulose nanofibre (CNF) derived from kenaf bast fibre and it's potential in enzyme immobilization support |
| title_full_unstemmed | Development of cellulose nanofibre (CNF) derived from kenaf bast fibre and it's potential in enzyme immobilization support |
| title_short | Development of cellulose nanofibre (CNF) derived from kenaf bast fibre and it's potential in enzyme immobilization support |
| title_sort | development of cellulose nanofibre cnf derived from kenaf bast fibre and it s potential in enzyme immobilization support |
| url | http://psasir.upm.edu.my/id/eprint/52028/1/Development%20of%20cellulose%20nanofibre%20%28CNF%29%20derived%20from%20kenaf%20bast%20fibre%20and%20it%27s%20potential%20in%20enzyme%20immobilization%20support.pdf |
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