Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars
Silicon (Si) is one of the most prevalent elements in the soil. It is beneficial for plant growth and development, and it contributes to plant defense against different stresses. The Lsi1 gene encodes a Si transporter that was identified in a mutant Japonica rice variety. This gene was not identifie...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52422/1/52422.pdf |
| _version_ | 1825930722307735552 |
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| author | Sahebi, Mahbod Musa, Mohamed Hanafi Yusop, Mohd Rafii Azizi, Parisa Abiri, Rambod Kalhori, Nahid Atabaki, Narges |
| author_facet | Sahebi, Mahbod Musa, Mohamed Hanafi Yusop, Mohd Rafii Azizi, Parisa Abiri, Rambod Kalhori, Nahid Atabaki, Narges |
| author_sort | Sahebi, Mahbod |
| collection | UPM |
| description | Silicon (Si) is one of the most prevalent elements in the soil. It is beneficial for plant growth and development, and it contributes to plant defense against different stresses. The Lsi1 gene encodes a Si transporter that was identified in a mutant Japonica rice variety. This gene was not identified in fourteen Malaysian rice varieties during screening. Then, a mutant version of Lsi1 was substituted for the native version in the three most common Malaysian rice varieties, MR219, MR220, and MR276, to evaluate the function of the transgene. Real-time PCR was used to explore the differential expression of Lsi1 in the three transgenic rice varieties. Silicon concentrations in the roots and leaves of transgenic plants were significantly higher than in wild-type plants. Transgenic varieties showed significant increases in the activities of the enzymes SOD, POD, APX, and CAT; photosynthesis; and chlorophyll content; however, the highest chlorophyll A and B levels were observed in transgenic MR276. Transgenic varieties have shown a stronger root and leaf structure, as well as hairier roots, compared to the wild-type plants. This suggests that Lsi1 plays a key role in rice, increasing the absorption and accumulation of Si, then alters antioxidant activities, and improves morphological properties. |
| first_indexed | 2024-03-06T09:15:53Z |
| format | Article |
| id | upm.eprints-52422 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:15:53Z |
| publishDate | 2017 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-524222017-06-06T08:43:33Z http://psasir.upm.edu.my/id/eprint/52422/ Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars Sahebi, Mahbod Musa, Mohamed Hanafi Yusop, Mohd Rafii Azizi, Parisa Abiri, Rambod Kalhori, Nahid Atabaki, Narges Silicon (Si) is one of the most prevalent elements in the soil. It is beneficial for plant growth and development, and it contributes to plant defense against different stresses. The Lsi1 gene encodes a Si transporter that was identified in a mutant Japonica rice variety. This gene was not identified in fourteen Malaysian rice varieties during screening. Then, a mutant version of Lsi1 was substituted for the native version in the three most common Malaysian rice varieties, MR219, MR220, and MR276, to evaluate the function of the transgene. Real-time PCR was used to explore the differential expression of Lsi1 in the three transgenic rice varieties. Silicon concentrations in the roots and leaves of transgenic plants were significantly higher than in wild-type plants. Transgenic varieties showed significant increases in the activities of the enzymes SOD, POD, APX, and CAT; photosynthesis; and chlorophyll content; however, the highest chlorophyll A and B levels were observed in transgenic MR276. Transgenic varieties have shown a stronger root and leaf structure, as well as hairier roots, compared to the wild-type plants. This suggests that Lsi1 plays a key role in rice, increasing the absorption and accumulation of Si, then alters antioxidant activities, and improves morphological properties. Hindawi Publishing Corporation 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52422/1/52422.pdf Sahebi, Mahbod and Musa, Mohamed Hanafi and Yusop, Mohd Rafii and Azizi, Parisa and Abiri, Rambod and Kalhori, Nahid and Atabaki, Narges (2017) Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars. BioMed Research International, 2017. art. no. 9064129. pp. 1-13. ISSN 2314-6133; ESSN: 2314-6141 https://www.hindawi.com/journals/bmri/2017/9064129/abs/ 10.1155/2017/9064129 |
| spellingShingle | Sahebi, Mahbod Musa, Mohamed Hanafi Yusop, Mohd Rafii Azizi, Parisa Abiri, Rambod Kalhori, Nahid Atabaki, Narges Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars |
| title | Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars |
| title_full | Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars |
| title_fullStr | Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars |
| title_full_unstemmed | Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars |
| title_short | Screening and expression of a silicon transporter gene (Lsi1) in wild-type indica rice cultivars |
| title_sort | screening and expression of a silicon transporter gene lsi1 in wild type indica rice cultivars |
| url | http://psasir.upm.edu.my/id/eprint/52422/1/52422.pdf |
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