Towards understanding pre-mRNA splicing mechanisms and the role of SR proteins
Alternative pre-mRNA splicing provides a source of vast protein diversity by removing non-coding sequences (introns) and accurately linking different exonic regions in the correct reading frame. The regulation of alternative splicing is essential for various cellular functions in both pathological a...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52434/1/Towards%20understanding%20pre-mRNA%20splicing%20mechanisms%20and%20the%20role%20of%20SR%20proteins.pdf |
| _version_ | 1796975749812453376 |
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| author | Sahebi, Mahbod Musa, Mohamed Hanafi Wijnen, Andre J. van Azizi, Parisa Abiri, Rambod Ashkani, Sadegh Taheri, Sima |
| author_facet | Sahebi, Mahbod Musa, Mohamed Hanafi Wijnen, Andre J. van Azizi, Parisa Abiri, Rambod Ashkani, Sadegh Taheri, Sima |
| author_sort | Sahebi, Mahbod |
| collection | UPM |
| description | Alternative pre-mRNA splicing provides a source of vast protein diversity by removing non-coding sequences (introns) and accurately linking different exonic regions in the correct reading frame. The regulation of alternative splicing is essential for various cellular functions in both pathological and physiological conditions. In eukaryotic cells, this process is commonly used to increase proteomic diversity and to control gene expression either co- or post-transcriptionally. Alternative splicing occurs within a megadalton-sized, multi-component machine consisting of RNA and proteins; during the splicing process, this complex undergoes dynamic changes via RNA–RNA, protein–protein and RNA–protein interactions. Co-transcriptional splicing functionally integrates the transcriptional machinery, thereby enabling the two processes to influence one another, whereas post-transcriptional splicing facilitates the coupling of RNA splicing with post-splicing events. This review addresses the structural aspects of spliceosomes and the mechanistic implications of their stepwise assembly on the regulation of pre-mRNA splicing. Moreover, the role of phosphorylation-based, signal-induced changes in the regulation of the splicing process is demonstrated. |
| first_indexed | 2024-03-06T09:15:55Z |
| format | Article |
| id | upm.eprints-52434 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:15:55Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-524342017-06-06T08:44:23Z http://psasir.upm.edu.my/id/eprint/52434/ Towards understanding pre-mRNA splicing mechanisms and the role of SR proteins Sahebi, Mahbod Musa, Mohamed Hanafi Wijnen, Andre J. van Azizi, Parisa Abiri, Rambod Ashkani, Sadegh Taheri, Sima Alternative pre-mRNA splicing provides a source of vast protein diversity by removing non-coding sequences (introns) and accurately linking different exonic regions in the correct reading frame. The regulation of alternative splicing is essential for various cellular functions in both pathological and physiological conditions. In eukaryotic cells, this process is commonly used to increase proteomic diversity and to control gene expression either co- or post-transcriptionally. Alternative splicing occurs within a megadalton-sized, multi-component machine consisting of RNA and proteins; during the splicing process, this complex undergoes dynamic changes via RNA–RNA, protein–protein and RNA–protein interactions. Co-transcriptional splicing functionally integrates the transcriptional machinery, thereby enabling the two processes to influence one another, whereas post-transcriptional splicing facilitates the coupling of RNA splicing with post-splicing events. This review addresses the structural aspects of spliceosomes and the mechanistic implications of their stepwise assembly on the regulation of pre-mRNA splicing. Moreover, the role of phosphorylation-based, signal-induced changes in the regulation of the splicing process is demonstrated. Elsevier 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52434/1/Towards%20understanding%20pre-mRNA%20splicing%20mechanisms%20and%20the%20role%20of%20SR%20proteins.pdf Sahebi, Mahbod and Musa, Mohamed Hanafi and Wijnen, Andre J. van and Azizi, Parisa and Abiri, Rambod and Ashkani, Sadegh and Taheri, Sima (2016) Towards understanding pre-mRNA splicing mechanisms and the role of SR proteins. Gene, 587 (2). pp. 107-119. ISSN 0378-1119; ESSN: 1879-0038 http://www.sciencedirect.com/science/article/pii/S0378111916303535 10.1016/j.gene.2016.04.057 |
| spellingShingle | Sahebi, Mahbod Musa, Mohamed Hanafi Wijnen, Andre J. van Azizi, Parisa Abiri, Rambod Ashkani, Sadegh Taheri, Sima Towards understanding pre-mRNA splicing mechanisms and the role of SR proteins |
| title | Towards understanding pre-mRNA splicing mechanisms and the role of SR proteins |
| title_full | Towards understanding pre-mRNA splicing mechanisms and the role of SR proteins |
| title_fullStr | Towards understanding pre-mRNA splicing mechanisms and the role of SR proteins |
| title_full_unstemmed | Towards understanding pre-mRNA splicing mechanisms and the role of SR proteins |
| title_short | Towards understanding pre-mRNA splicing mechanisms and the role of SR proteins |
| title_sort | towards understanding pre mrna splicing mechanisms and the role of sr proteins |
| url | http://psasir.upm.edu.my/id/eprint/52434/1/Towards%20understanding%20pre-mRNA%20splicing%20mechanisms%20and%20the%20role%20of%20SR%20proteins.pdf |
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