Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants
In the present study, we have reported a simple, fast and efficient regeneration protocol using mature embryos as explants, and discovered its effective applicability to a range of Indica rice genotypes. We have considered the response of six varieties in the steps of the regeneration procedure. The...
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| Format: | Article |
| Language: | English |
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Elsevier Masson
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52453/1/Highly%20efficient%20protocol%20for%20callogenesis%2C%20somagenesis%20and%20regeneration%20of%20Indica%20rice%20plants.pdf |
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| author | Azizi, Parisa Yusop, Mohd Rafii Mahmood, Maziah Musa, Mohamed Hanafi Abdullah, Siti Nor Akmar Abiri, Rambod Sahebi, Mahbod |
| author_facet | Azizi, Parisa Yusop, Mohd Rafii Mahmood, Maziah Musa, Mohamed Hanafi Abdullah, Siti Nor Akmar Abiri, Rambod Sahebi, Mahbod |
| author_sort | Azizi, Parisa |
| collection | UPM |
| description | In the present study, we have reported a simple, fast and efficient regeneration protocol using mature embryos as explants, and discovered its effective applicability to a range of Indica rice genotypes. We have considered the response of six varieties in the steps of the regeneration procedure. The results showed that calli were variably developed from the scutellar region of seeds and visible within 6–20 days. The highest and lowest calli induction frequency (70% and 51.66%) and number of induced calli from seeds (14 and 10.33) were observed in MR269 and MRQ74, respectively. The maximum and minimum number (7.66 and 4) and frequency of embryogenic calli (38.33% and 20%) were recorded in MR219 and MRQ74, respectively. However, the highest browning rate was observed in MR84 (87%) and the lowest rate in MRQ50 (46%). The majority of plants regenerated from embryogenic calli were obtained from MRQ50 (54%) and the minimum number of plants from MR84. In this study, the maximum numbers of plantlets were regenerated from the varieties with highest rate of embryogenic calli. Also, various varieties, including MRQ50, MR269, MR276 and MR219, were satisfactorily responding, while MRQ74 and MR84 weakly responded to the procedure. Such a simple, successful and generalized method possesses the potential to become an important tool for crop improvement and functional studies of genes in rice as a model monocot plant. |
| first_indexed | 2024-03-06T09:15:59Z |
| format | Article |
| id | upm.eprints-52453 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:15:59Z |
| publishDate | 2015 |
| publisher | Elsevier Masson |
| record_format | dspace |
| spelling | upm.eprints-524532017-06-06T09:10:52Z http://psasir.upm.edu.my/id/eprint/52453/ Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants Azizi, Parisa Yusop, Mohd Rafii Mahmood, Maziah Musa, Mohamed Hanafi Abdullah, Siti Nor Akmar Abiri, Rambod Sahebi, Mahbod In the present study, we have reported a simple, fast and efficient regeneration protocol using mature embryos as explants, and discovered its effective applicability to a range of Indica rice genotypes. We have considered the response of six varieties in the steps of the regeneration procedure. The results showed that calli were variably developed from the scutellar region of seeds and visible within 6–20 days. The highest and lowest calli induction frequency (70% and 51.66%) and number of induced calli from seeds (14 and 10.33) were observed in MR269 and MRQ74, respectively. The maximum and minimum number (7.66 and 4) and frequency of embryogenic calli (38.33% and 20%) were recorded in MR219 and MRQ74, respectively. However, the highest browning rate was observed in MR84 (87%) and the lowest rate in MRQ50 (46%). The majority of plants regenerated from embryogenic calli were obtained from MRQ50 (54%) and the minimum number of plants from MR84. In this study, the maximum numbers of plantlets were regenerated from the varieties with highest rate of embryogenic calli. Also, various varieties, including MRQ50, MR269, MR276 and MR219, were satisfactorily responding, while MRQ74 and MR84 weakly responded to the procedure. Such a simple, successful and generalized method possesses the potential to become an important tool for crop improvement and functional studies of genes in rice as a model monocot plant. Elsevier Masson 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52453/1/Highly%20efficient%20protocol%20for%20callogenesis%2C%20somagenesis%20and%20regeneration%20of%20Indica%20rice%20plants.pdf Azizi, Parisa and Yusop, Mohd Rafii and Mahmood, Maziah and Musa, Mohamed Hanafi and Abdullah, Siti Nor Akmar and Abiri, Rambod and Sahebi, Mahbod (2015) Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants. Comptes Rendus Biologies, 338 (7). pp. 463-470. ISSN 1631-0691; ESSN: 1768-3238 http://www.sciencedirect.com/science/article/pii/S1631069115000979 10.1016/j.crvi.2015.04.004 |
| spellingShingle | Azizi, Parisa Yusop, Mohd Rafii Mahmood, Maziah Musa, Mohamed Hanafi Abdullah, Siti Nor Akmar Abiri, Rambod Sahebi, Mahbod Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants |
| title | Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants |
| title_full | Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants |
| title_fullStr | Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants |
| title_full_unstemmed | Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants |
| title_short | Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica rice plants |
| title_sort | highly efficient protocol for callogenesis somagenesis and regeneration of indica rice plants |
| url | http://psasir.upm.edu.my/id/eprint/52453/1/Highly%20efficient%20protocol%20for%20callogenesis%2C%20somagenesis%20and%20regeneration%20of%20Indica%20rice%20plants.pdf |
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