Hypo-osmotic swelling test modification to enhance cell membrane integrity evaluation in cryopreserved bull semen
The objective of this study was to enhance the hypo-osmotic swelling test evaluation when it reads under light microscope using 5% formaldehyde-fixed sperm solution buffer (FSSB). Twenty four ejaculates were collected from six crossbred bulls using electro-ejaculator (EE). Tris-egg yolk extender was...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52546/1/04%20JTAS-0872-2016-6thProof.pdf |
| _version_ | 1825930746767867904 |
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| author | Baiee, Falah Hasan Ali Haron, Abd Wahid Yusoff, Rosnina Omar, Mohamed Ariff Degu, Nurhusien Yimer Ghareeb, Salman Hammadi Abdelbagi, Tarig Abdelrouf Ahmedeltayeb Mada, Khumran Armiya'u |
| author_facet | Baiee, Falah Hasan Ali Haron, Abd Wahid Yusoff, Rosnina Omar, Mohamed Ariff Degu, Nurhusien Yimer Ghareeb, Salman Hammadi Abdelbagi, Tarig Abdelrouf Ahmedeltayeb Mada, Khumran Armiya'u |
| author_sort | Baiee, Falah Hasan Ali |
| collection | UPM |
| description | The objective of this study was to enhance the hypo-osmotic swelling test evaluation when it reads under light microscope using 5% formaldehyde-fixed sperm solution buffer (FSSB). Twenty four ejaculates were collected from six crossbred bulls using electro-ejaculator (EE). Tris-egg yolk extender was used to cryopreserve the semen. Concentration, volume, motility, morphology and viability rates of fresh semen were evaluated and samples were cryopreserved in liquid nitrogen. After two weeks of liquid nitrogen treatment (freezing), the motility, morphology and viability rate of the semen were evaluated. In order to carry out a hypo-osmotic swelling test, post-thaw semen was divided into four aliquots based on period of incubation (30 or 60 minutes) adding FSSB to half the samples. The components of FSSB were 5% formaldehyde and 1% eosin-nigrosin stain in PBS. Results showed that F (61.48 ±0.89%) resulted in higher percentage (P<0.05) of total membrane intact compared with F 60 30 (54.40 ±1.34%) and N 30 (53.96±1.17%), but did not differ (P>0.05) with N60 (60.90±0.70%). In conclusion, adding 10 µl of FSSB after 60 minute of incubation with hypo-osmotic swelling solution (HOSS) will enhance evaluation of the hypo-osmotic swelling test (HOST) under light microscope. |
| first_indexed | 2024-03-06T09:16:15Z |
| format | Article |
| id | upm.eprints-52546 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:16:15Z |
| publishDate | 2017 |
| publisher | Universiti Putra Malaysia Press |
| record_format | dspace |
| spelling | upm.eprints-525462017-06-07T03:01:52Z http://psasir.upm.edu.my/id/eprint/52546/ Hypo-osmotic swelling test modification to enhance cell membrane integrity evaluation in cryopreserved bull semen Baiee, Falah Hasan Ali Haron, Abd Wahid Yusoff, Rosnina Omar, Mohamed Ariff Degu, Nurhusien Yimer Ghareeb, Salman Hammadi Abdelbagi, Tarig Abdelrouf Ahmedeltayeb Mada, Khumran Armiya'u The objective of this study was to enhance the hypo-osmotic swelling test evaluation when it reads under light microscope using 5% formaldehyde-fixed sperm solution buffer (FSSB). Twenty four ejaculates were collected from six crossbred bulls using electro-ejaculator (EE). Tris-egg yolk extender was used to cryopreserve the semen. Concentration, volume, motility, morphology and viability rates of fresh semen were evaluated and samples were cryopreserved in liquid nitrogen. After two weeks of liquid nitrogen treatment (freezing), the motility, morphology and viability rate of the semen were evaluated. In order to carry out a hypo-osmotic swelling test, post-thaw semen was divided into four aliquots based on period of incubation (30 or 60 minutes) adding FSSB to half the samples. The components of FSSB were 5% formaldehyde and 1% eosin-nigrosin stain in PBS. Results showed that F (61.48 ±0.89%) resulted in higher percentage (P<0.05) of total membrane intact compared with F 60 30 (54.40 ±1.34%) and N 30 (53.96±1.17%), but did not differ (P>0.05) with N60 (60.90±0.70%). In conclusion, adding 10 µl of FSSB after 60 minute of incubation with hypo-osmotic swelling solution (HOSS) will enhance evaluation of the hypo-osmotic swelling test (HOST) under light microscope. Universiti Putra Malaysia Press 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52546/1/04%20JTAS-0872-2016-6thProof.pdf Baiee, Falah Hasan Ali and Haron, Abd Wahid and Yusoff, Rosnina and Omar, Mohamed Ariff and Degu, Nurhusien Yimer and Ghareeb, Salman Hammadi and Abdelbagi, Tarig Abdelrouf Ahmedeltayeb and Mada, Khumran Armiya'u (2017) Hypo-osmotic swelling test modification to enhance cell membrane integrity evaluation in cryopreserved bull semen. Pertanika Journal of Tropical Agricultural Science, 40 (2). pp. 257-268. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2040%20(2)%20May.%202017/04%20JTAS-0872-2016-6thProof.pdf |
| spellingShingle | Baiee, Falah Hasan Ali Haron, Abd Wahid Yusoff, Rosnina Omar, Mohamed Ariff Degu, Nurhusien Yimer Ghareeb, Salman Hammadi Abdelbagi, Tarig Abdelrouf Ahmedeltayeb Mada, Khumran Armiya'u Hypo-osmotic swelling test modification to enhance cell membrane integrity evaluation in cryopreserved bull semen |
| title | Hypo-osmotic swelling test modification to enhance cell membrane integrity evaluation in cryopreserved bull semen |
| title_full | Hypo-osmotic swelling test modification to enhance cell membrane integrity evaluation in cryopreserved bull semen |
| title_fullStr | Hypo-osmotic swelling test modification to enhance cell membrane integrity evaluation in cryopreserved bull semen |
| title_full_unstemmed | Hypo-osmotic swelling test modification to enhance cell membrane integrity evaluation in cryopreserved bull semen |
| title_short | Hypo-osmotic swelling test modification to enhance cell membrane integrity evaluation in cryopreserved bull semen |
| title_sort | hypo osmotic swelling test modification to enhance cell membrane integrity evaluation in cryopreserved bull semen |
| url | http://psasir.upm.edu.my/id/eprint/52546/1/04%20JTAS-0872-2016-6thProof.pdf |
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