RNA from fresh frozen cryosections of oil palm inflorescences is superior to FFPE sections
Formalin-fixed, paraffin-embedded (FFPE) and fresh frozen (FF) tissue sections are valuable sources for histology and molecular studies. However, FFPE tissue sections usually provide poor quality RNA that is unsuitable for most downstream molecular applications. In this study, tissue preparation for...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Malaysian Palm Oil Board
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/52905/1/RNA%20from%20fresh%20frozen%20cryosections%20of%20oil%20palm%20inflorescences%20is%20superior%20to%20FFPE%20sections.pdf |
| _version_ | 1825930775053205504 |
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| author | Chai, Sook Keat Namasivayam, Parameswari Ho, Chai Ling Abdul Aziz, Norazlin Abdullah, Meilina Ong Ooi, Siew Eng |
| author_facet | Chai, Sook Keat Namasivayam, Parameswari Ho, Chai Ling Abdul Aziz, Norazlin Abdullah, Meilina Ong Ooi, Siew Eng |
| author_sort | Chai, Sook Keat |
| collection | UPM |
| description | Formalin-fixed, paraffin-embedded (FFPE) and fresh frozen (FF) tissue sections are valuable sources for histology and molecular studies. However, FFPE tissue sections usually provide poor quality RNA that is unsuitable for most downstream molecular applications. In this study, tissue preparation for cryosectioning and RNA extraction protocols were optimised for FF oil palm inflorescences. FF tissue treated with sucrose offered good cellular image resolution and more importantly, good RNA quality. Higher RNA quality with good RIN values (>6) was obtained from FF sections compared to the fragmented RNA from FFPE tissue sections. However, FFPE sections provided better resolution in terms of cellular morphology. The quality of cellular morphology of the FF sections was moderate, but was sufficient to distinguish the different cell types. Hence, FF oil palm inflorescences is a better choice for downstream transcriptomics studies that require tissue sections without compromising the cytological details. |
| first_indexed | 2024-03-06T09:16:41Z |
| format | Article |
| id | upm.eprints-52905 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:16:41Z |
| publishDate | 2016 |
| publisher | Malaysian Palm Oil Board |
| record_format | dspace |
| spelling | upm.eprints-529052022-03-16T08:31:33Z http://psasir.upm.edu.my/id/eprint/52905/ RNA from fresh frozen cryosections of oil palm inflorescences is superior to FFPE sections Chai, Sook Keat Namasivayam, Parameswari Ho, Chai Ling Abdul Aziz, Norazlin Abdullah, Meilina Ong Ooi, Siew Eng Formalin-fixed, paraffin-embedded (FFPE) and fresh frozen (FF) tissue sections are valuable sources for histology and molecular studies. However, FFPE tissue sections usually provide poor quality RNA that is unsuitable for most downstream molecular applications. In this study, tissue preparation for cryosectioning and RNA extraction protocols were optimised for FF oil palm inflorescences. FF tissue treated with sucrose offered good cellular image resolution and more importantly, good RNA quality. Higher RNA quality with good RIN values (>6) was obtained from FF sections compared to the fragmented RNA from FFPE tissue sections. However, FFPE sections provided better resolution in terms of cellular morphology. The quality of cellular morphology of the FF sections was moderate, but was sufficient to distinguish the different cell types. Hence, FF oil palm inflorescences is a better choice for downstream transcriptomics studies that require tissue sections without compromising the cytological details. Malaysian Palm Oil Board 2016-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/52905/1/RNA%20from%20fresh%20frozen%20cryosections%20of%20oil%20palm%20inflorescences%20is%20superior%20to%20FFPE%20sections.pdf Chai, Sook Keat and Namasivayam, Parameswari and Ho, Chai Ling and Abdul Aziz, Norazlin and Abdullah, Meilina Ong and Ooi, Siew Eng (2016) RNA from fresh frozen cryosections of oil palm inflorescences is superior to FFPE sections. Journal of Oil Palm Research, 28 (2). pp. 154-160. ISSN 1511-2780 http://jopr.mpob.gov.my/rna-from-fresh-frozen-cryosections-of-oil-palm-inflorescences-is-superior-to-ffpe-sections/ |
| spellingShingle | Chai, Sook Keat Namasivayam, Parameswari Ho, Chai Ling Abdul Aziz, Norazlin Abdullah, Meilina Ong Ooi, Siew Eng RNA from fresh frozen cryosections of oil palm inflorescences is superior to FFPE sections |
| title | RNA from fresh frozen cryosections of oil palm inflorescences is superior to FFPE sections |
| title_full | RNA from fresh frozen cryosections of oil palm inflorescences is superior to FFPE sections |
| title_fullStr | RNA from fresh frozen cryosections of oil palm inflorescences is superior to FFPE sections |
| title_full_unstemmed | RNA from fresh frozen cryosections of oil palm inflorescences is superior to FFPE sections |
| title_short | RNA from fresh frozen cryosections of oil palm inflorescences is superior to FFPE sections |
| title_sort | rna from fresh frozen cryosections of oil palm inflorescences is superior to ffpe sections |
| url | http://psasir.upm.edu.my/id/eprint/52905/1/RNA%20from%20fresh%20frozen%20cryosections%20of%20oil%20palm%20inflorescences%20is%20superior%20to%20FFPE%20sections.pdf |
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