The in vitro therapeutic activity of ellagic acid-alginate-silver nanopeprintss on breast cancer cells (MCF-7) and normal fibroblast cells (3T3)
The present work involves the development of EA-Alg-AgNPs nanocomposite based on ellagic acid (EA) as active compound. Silver nitrate was taken as the metal precursor (AgNPs) and sodium alginate (Alg) as a reducing agent. The EA-Alg-AgNPs nanocomposite was characterized using transmission electron m...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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American Scientific Publishers
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53027/1/The%20in%20vitro%20therapeutic%20activity%20of%20ellagic%20acid-alginate-silver%20nanopeprintss%20on%20breast%20cancer%20cells%20%28MCF-7%29%20and%20normal%20fibroblast%20cells%20%283T3%29.pdf |
| _version_ | 1796975820108988416 |
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| author | Hussein-Al-Ali, Samer Hasan Balavandy, Sepideh Keshan Abidin, Zurina Zainal Kura, Aminu Umar Fakurazi, Sharida Hussein, Mohd Zobir Silwadi, Mohammed Fawaz |
| author_facet | Hussein-Al-Ali, Samer Hasan Balavandy, Sepideh Keshan Abidin, Zurina Zainal Kura, Aminu Umar Fakurazi, Sharida Hussein, Mohd Zobir Silwadi, Mohammed Fawaz |
| author_sort | Hussein-Al-Ali, Samer Hasan |
| collection | UPM |
| description | The present work involves the development of EA-Alg-AgNPs nanocomposite based on ellagic acid (EA) as active compound. Silver nitrate was taken as the metal precursor (AgNPs) and sodium alginate (Alg) as a reducing agent. The EA-Alg-AgNPs nanocomposite was characterized using transmission electron microscopy (TEM), zeta potential, and in vitro release kinetics. The particles thus obtained were spherical in shape and having an average particles size of 10 nm, zeta potentials of –8.2 mV, and the release kinetics of EA from nanocomposite was following Hixson-Crowell kinetics models with R 2 = 0.9956. The cytotoxicity potential of free EA, Alg-AgNPs and the EA-Alg-AgNPs nanocomposite may be determined using a normal mouse fibroblast cells (3T3) and breast cancer cells (MCF-7). EA-Alg-AgNPs nanocomposite demonstrated a increased cytotoxicity effect when compared to free EA on MCF-7 cells with 15.3% cell viability at 128 μg/mL; compared to 33.5% cell viability in a direct EA exposure. It is worth mentioning the cytotoxicity of Alg-AgNPs against MCF-7 shows 28% viability at 128 μg/mL. |
| first_indexed | 2024-03-06T09:16:57Z |
| format | Article |
| id | upm.eprints-53027 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:16:57Z |
| publishDate | 2016 |
| publisher | American Scientific Publishers |
| record_format | dspace |
| spelling | upm.eprints-530272017-11-17T02:41:48Z http://psasir.upm.edu.my/id/eprint/53027/ The in vitro therapeutic activity of ellagic acid-alginate-silver nanopeprintss on breast cancer cells (MCF-7) and normal fibroblast cells (3T3) Hussein-Al-Ali, Samer Hasan Balavandy, Sepideh Keshan Abidin, Zurina Zainal Kura, Aminu Umar Fakurazi, Sharida Hussein, Mohd Zobir Silwadi, Mohammed Fawaz The present work involves the development of EA-Alg-AgNPs nanocomposite based on ellagic acid (EA) as active compound. Silver nitrate was taken as the metal precursor (AgNPs) and sodium alginate (Alg) as a reducing agent. The EA-Alg-AgNPs nanocomposite was characterized using transmission electron microscopy (TEM), zeta potential, and in vitro release kinetics. The particles thus obtained were spherical in shape and having an average particles size of 10 nm, zeta potentials of –8.2 mV, and the release kinetics of EA from nanocomposite was following Hixson-Crowell kinetics models with R 2 = 0.9956. The cytotoxicity potential of free EA, Alg-AgNPs and the EA-Alg-AgNPs nanocomposite may be determined using a normal mouse fibroblast cells (3T3) and breast cancer cells (MCF-7). EA-Alg-AgNPs nanocomposite demonstrated a increased cytotoxicity effect when compared to free EA on MCF-7 cells with 15.3% cell viability at 128 μg/mL; compared to 33.5% cell viability in a direct EA exposure. It is worth mentioning the cytotoxicity of Alg-AgNPs against MCF-7 shows 28% viability at 128 μg/mL. American Scientific Publishers 2016-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53027/1/The%20in%20vitro%20therapeutic%20activity%20of%20ellagic%20acid-alginate-silver%20nanopeprintss%20on%20breast%20cancer%20cells%20%28MCF-7%29%20and%20normal%20fibroblast%20cells%20%283T3%29.pdf Hussein-Al-Ali, Samer Hasan and Balavandy, Sepideh Keshan and Abidin, Zurina Zainal and Kura, Aminu Umar and Fakurazi, Sharida and Hussein, Mohd Zobir and Silwadi, Mohammed Fawaz (2016) The in vitro therapeutic activity of ellagic acid-alginate-silver nanopeprintss on breast cancer cells (MCF-7) and normal fibroblast cells (3T3). Science of Advanced Materials, 8 (3). pp. 545-553. ISSN 1947-2935 http://www.aspbs.com/sam.htm 10.1166/sam.2016.2673 |
| spellingShingle | Hussein-Al-Ali, Samer Hasan Balavandy, Sepideh Keshan Abidin, Zurina Zainal Kura, Aminu Umar Fakurazi, Sharida Hussein, Mohd Zobir Silwadi, Mohammed Fawaz The in vitro therapeutic activity of ellagic acid-alginate-silver nanopeprintss on breast cancer cells (MCF-7) and normal fibroblast cells (3T3) |
| title | The in vitro therapeutic activity of ellagic acid-alginate-silver nanopeprintss on breast cancer cells (MCF-7) and normal fibroblast cells (3T3) |
| title_full | The in vitro therapeutic activity of ellagic acid-alginate-silver nanopeprintss on breast cancer cells (MCF-7) and normal fibroblast cells (3T3) |
| title_fullStr | The in vitro therapeutic activity of ellagic acid-alginate-silver nanopeprintss on breast cancer cells (MCF-7) and normal fibroblast cells (3T3) |
| title_full_unstemmed | The in vitro therapeutic activity of ellagic acid-alginate-silver nanopeprintss on breast cancer cells (MCF-7) and normal fibroblast cells (3T3) |
| title_short | The in vitro therapeutic activity of ellagic acid-alginate-silver nanopeprintss on breast cancer cells (MCF-7) and normal fibroblast cells (3T3) |
| title_sort | in vitro therapeutic activity of ellagic acid alginate silver nanopeprintss on breast cancer cells mcf 7 and normal fibroblast cells 3t3 |
| url | http://psasir.upm.edu.my/id/eprint/53027/1/The%20in%20vitro%20therapeutic%20activity%20of%20ellagic%20acid-alginate-silver%20nanopeprintss%20on%20breast%20cancer%20cells%20%28MCF-7%29%20and%20normal%20fibroblast%20cells%20%283T3%29.pdf |
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