Modeling and optimization of lipase-catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor
Response surface methodology (RSM) was employed to optimize the process variables namely packed bed height (cm) and flow rates (ml/min) on diacylglycerol (DAG) production via partial hydrolysis of palm oil using immobilized Rhizomucor miehei lipase in packed bed reactor (PBR). Quadratic models were...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Walter de Gruyter GmbH
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53302/1/Modeling%20and%20optimization%20of%20lipase-catalyzed%20partial%20hydrolysis%20for%20diacylglycerol%20production%20in%20packed%20bed%20reactor.pdf |
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| author | Phuah, Eng Tong Lee, Yee Ying Tang, Teck Kim Lai, Oi Ming Choong, Thomas Shean Yaw Tan, Chin Ping Ng, Wee Nak Lo, Seong Koon |
| author_facet | Phuah, Eng Tong Lee, Yee Ying Tang, Teck Kim Lai, Oi Ming Choong, Thomas Shean Yaw Tan, Chin Ping Ng, Wee Nak Lo, Seong Koon |
| author_sort | Phuah, Eng Tong |
| collection | UPM |
| description | Response surface methodology (RSM) was employed to optimize the process variables namely packed bed height (cm) and flow rates (ml/min) on diacylglycerol (DAG) production via partial hydrolysis of palm oil using immobilized Rhizomucor miehei lipase in packed bed reactor (PBR). Quadratic models were successfully developed for both DAG(y) and unhydrolyzed triacylglycerol ((un)TAG) with determination coefficient (R2) of 0.9931 and 0.9986, respectively coupled with insignificant lack of fit (p > 0.05). Optimal conditions for DAG synthesis were evaluated to be 10 cm packed bed height and 3.8 ml/min flow rate. Immobilized enzyme can be reused up to 10 times without significant changes in enzymatic activity. The partial hydrolysis under studied was found to be mass transfer-controlled. |
| first_indexed | 2024-03-06T09:17:29Z |
| format | Article |
| id | upm.eprints-53302 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:17:29Z |
| publishDate | 2016 |
| publisher | Walter de Gruyter GmbH |
| record_format | dspace |
| spelling | upm.eprints-533022022-03-18T05:01:59Z http://psasir.upm.edu.my/id/eprint/53302/ Modeling and optimization of lipase-catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor Phuah, Eng Tong Lee, Yee Ying Tang, Teck Kim Lai, Oi Ming Choong, Thomas Shean Yaw Tan, Chin Ping Ng, Wee Nak Lo, Seong Koon Response surface methodology (RSM) was employed to optimize the process variables namely packed bed height (cm) and flow rates (ml/min) on diacylglycerol (DAG) production via partial hydrolysis of palm oil using immobilized Rhizomucor miehei lipase in packed bed reactor (PBR). Quadratic models were successfully developed for both DAG(y) and unhydrolyzed triacylglycerol ((un)TAG) with determination coefficient (R2) of 0.9931 and 0.9986, respectively coupled with insignificant lack of fit (p > 0.05). Optimal conditions for DAG synthesis were evaluated to be 10 cm packed bed height and 3.8 ml/min flow rate. Immobilized enzyme can be reused up to 10 times without significant changes in enzymatic activity. The partial hydrolysis under studied was found to be mass transfer-controlled. Walter de Gruyter GmbH 2016-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/53302/1/Modeling%20and%20optimization%20of%20lipase-catalyzed%20partial%20hydrolysis%20for%20diacylglycerol%20production%20in%20packed%20bed%20reactor.pdf Phuah, Eng Tong and Lee, Yee Ying and Tang, Teck Kim and Lai, Oi Ming and Choong, Thomas Shean Yaw and Tan, Chin Ping and Ng, Wee Nak and Lo, Seong Koon (2016) Modeling and optimization of lipase-catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor. International Journal of Food Engineering, 12 (7). pp. 681-689. ISSN 2194-5764; ESSN: 1556-3758 https://www.degruyter.com/document/doi/10.1515/ijfe-2015-0381/html 10.1515/ijfe-2015-0381 |
| spellingShingle | Phuah, Eng Tong Lee, Yee Ying Tang, Teck Kim Lai, Oi Ming Choong, Thomas Shean Yaw Tan, Chin Ping Ng, Wee Nak Lo, Seong Koon Modeling and optimization of lipase-catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor |
| title | Modeling and optimization of lipase-catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor |
| title_full | Modeling and optimization of lipase-catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor |
| title_fullStr | Modeling and optimization of lipase-catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor |
| title_full_unstemmed | Modeling and optimization of lipase-catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor |
| title_short | Modeling and optimization of lipase-catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor |
| title_sort | modeling and optimization of lipase catalyzed partial hydrolysis for diacylglycerol production in packed bed reactor |
| url | http://psasir.upm.edu.my/id/eprint/53302/1/Modeling%20and%20optimization%20of%20lipase-catalyzed%20partial%20hydrolysis%20for%20diacylglycerol%20production%20in%20packed%20bed%20reactor.pdf |
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