Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia
Curvularia species are important phytopathogens reported worldwide. They are closely related; consist of major destructive pathogens mainly for grasses and cereal plants including rice (Oryza sativa). A leaf spot symptom of rice is one of the common symptoms found in the rice field and caused reduct...
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| Format: | Article |
| Language: | English |
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Springer - Verlag Italia Srl
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/53336/1/Morphological%20and%20molecular%20characterization%20of%20Curvularia%20and%20related%20species%20associated%20with%20leaf%20spot%20disease%20of%20rice%20in%20Peninsular%20Malaysia.pdf |
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| author | Kusai, Nor Azizah Mior Zakuan Azmi, Madihah Zulkifly, Shahrizim Yusof, Mohd Termizi Mohd Zainudin, Nur Ain Izzati |
| author_facet | Kusai, Nor Azizah Mior Zakuan Azmi, Madihah Zulkifly, Shahrizim Yusof, Mohd Termizi Mohd Zainudin, Nur Ain Izzati |
| author_sort | Kusai, Nor Azizah |
| collection | UPM |
| description | Curvularia species are important phytopathogens reported worldwide. They are closely related; consist of major destructive pathogens mainly for grasses and cereal plants including rice (Oryza sativa). A leaf spot symptom of rice is one of the common symptoms found in the rice field and caused reduction of rice yield. However, there are no reports on Curvularia species associated with rice leaves showing spot symptoms. The objectives are to isolate and characterize Curvularia and related species from leaf spot of rice by using morphological and molecular characterization and to determine the phylogenetic relationship between the isolated fungi. Fungal isolation was done from diseased rice leaves showing leaf spot symptoms collected throughout Peninsular Malaysia. Thirty-three isolates were recovered and identified based on their morphological characteristics such as conidia morphology, colony appearance, pigmentation and growth rate for species delimitation. Internal transcribed spacer (ITS) region was amplified to confirm the species identification. The 33 isolates were identified as Bipolaris sorokiniana (10 isolates), Curvularia hawaiiensis (8 isolates), C. geniculata (6 isolates), C. eragrostidis (6 isolates), C. aeria (2 isolates) and C. lunata (1 isolate). A phylogenetic tree was constructed based on ITS sequences using neighbour-joining method. The tree grouped members of Curvularia and Bipolaris into different clades. The phylogenetic tree indicated that the presence of two groups of fungi species; highly virulent and mild pathogens. In conclusion, Curvularia species and Bipolaris sorokiniana were present in rice field in Malaysia and associated with leaf spot of rice. |
| first_indexed | 2024-03-06T09:17:34Z |
| format | Article |
| id | upm.eprints-53336 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:17:34Z |
| publishDate | 2016 |
| publisher | Springer - Verlag Italia Srl |
| record_format | dspace |
| spelling | upm.eprints-533362017-10-11T10:31:12Z http://psasir.upm.edu.my/id/eprint/53336/ Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia Kusai, Nor Azizah Mior Zakuan Azmi, Madihah Zulkifly, Shahrizim Yusof, Mohd Termizi Mohd Zainudin, Nur Ain Izzati Curvularia species are important phytopathogens reported worldwide. They are closely related; consist of major destructive pathogens mainly for grasses and cereal plants including rice (Oryza sativa). A leaf spot symptom of rice is one of the common symptoms found in the rice field and caused reduction of rice yield. However, there are no reports on Curvularia species associated with rice leaves showing spot symptoms. The objectives are to isolate and characterize Curvularia and related species from leaf spot of rice by using morphological and molecular characterization and to determine the phylogenetic relationship between the isolated fungi. Fungal isolation was done from diseased rice leaves showing leaf spot symptoms collected throughout Peninsular Malaysia. Thirty-three isolates were recovered and identified based on their morphological characteristics such as conidia morphology, colony appearance, pigmentation and growth rate for species delimitation. Internal transcribed spacer (ITS) region was amplified to confirm the species identification. The 33 isolates were identified as Bipolaris sorokiniana (10 isolates), Curvularia hawaiiensis (8 isolates), C. geniculata (6 isolates), C. eragrostidis (6 isolates), C. aeria (2 isolates) and C. lunata (1 isolate). A phylogenetic tree was constructed based on ITS sequences using neighbour-joining method. The tree grouped members of Curvularia and Bipolaris into different clades. The phylogenetic tree indicated that the presence of two groups of fungi species; highly virulent and mild pathogens. In conclusion, Curvularia species and Bipolaris sorokiniana were present in rice field in Malaysia and associated with leaf spot of rice. Springer - Verlag Italia Srl 2016-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53336/1/Morphological%20and%20molecular%20characterization%20of%20Curvularia%20and%20related%20species%20associated%20with%20leaf%20spot%20disease%20of%20rice%20in%20Peninsular%20Malaysia.pdf Kusai, Nor Azizah and Mior Zakuan Azmi, Madihah and Zulkifly, Shahrizim and Yusof, Mohd Termizi and Mohd Zainudin, Nur Ain Izzati (2016) Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia. Rendiconti Lincei. Scienze Fisiche e Naturali, 27 (2). pp. 205-214. ISSN 2037-4631; ESSN: 1720-0776 https://link.springer.com/article/10.1007/s12210-015-0458-6 10.1007/s12210-015-0458-6 |
| spellingShingle | Kusai, Nor Azizah Mior Zakuan Azmi, Madihah Zulkifly, Shahrizim Yusof, Mohd Termizi Mohd Zainudin, Nur Ain Izzati Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia |
| title | Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia |
| title_full | Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia |
| title_fullStr | Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia |
| title_full_unstemmed | Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia |
| title_short | Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia |
| title_sort | morphological and molecular characterization of curvularia and related species associated with leaf spot disease of rice in peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/53336/1/Morphological%20and%20molecular%20characterization%20of%20Curvularia%20and%20related%20species%20associated%20with%20leaf%20spot%20disease%20of%20rice%20in%20Peninsular%20Malaysia.pdf |
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